Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,869	C→T	100%	intergenic (‑293/‑110)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,869	0	C→T	100.0%	86.8	42	intergenic (‑293/‑110)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (6/33):  ref base (0/0):  total (8/34)

AACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GT...C...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGCG  >  NC_014034/1732790‑1732949                                                                                           |                                                                                        aaCAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGa                                                                <  1:3845554/101‑1 (MQ=21)    aGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGc                                                             <  2:991574/101‑1 (MQ=21)              ccggtttttCCTAGGAAAAATACGCCTTTCTTTCTTTTCGGGGGGGCGGGGGGTGAACCCCC...C.C....GC...GGGTCG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcc                                                   <  2:70871/96‑1 (MQ=17)               ccGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GCGGGT...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTccc                                                  <  1:3610843/101‑1 (MQ=21)                  cTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcccccc                                               <  2:3336542/101‑1 (MQ=18)                  cTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTcccccc                                               <  2:5781576/101‑1 (MQ=21)                     tcccTGGGCAAAATCCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTg                                            <  2:3229874/99‑1 (MQ=17)                       ccgaGGAAAAATTCGCCCTTTTTGCTTTGGGGGGGGGCGGGGGGGCAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaa                                          <  1:599072/98‑1 (MQ=2)                       ccaaGGAAAAATCCGCCTTTTTTTCTTTTGGGGGGGGCGGGGGGGAAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaa                                          <  2:335494/98‑1 (MQ=9)                        cTAGGCAAAATCCCCCCTTTTTTCTTTGGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGaaa                                         <  1:2496240/101‑1 (MQ=14)                          agtcAAAATCCTCCTTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAAc                                       <  2:2537918/98‑1 (MQ=17)                            gCAAAGTACCCCCTTTTTTTTTTTCGGGGGGGGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:5385751/101‑1 (MQ=17)                            gCAAAATACGCCCTTCTTTCTTGTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:1652602/101‑1 (MQ=18)                             taaaaTCCGCCCTCGTTTCTTTTGGGGGGGGCGGGAGGTGAAGCCCCTGGC.G....GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:2685915/100‑1 (MQ=12)                             cAAAATCCCCCTTTTTTTTTTTGGGGGGGGGGGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  1:2407497/101‑1 (MQ=2)                             cAAAATCCCCCTTTCTTTTTTTGGGGGGGGGGGGGGGGTGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:5068540/101‑1 (MQ=9)                             aaaattccGCCCTTCTTTCTTTGGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCtt                                    <  2:1954077/94‑1 (MQ=14)                             aaaaactcccccTTTTTTTTTTGGGGGGGGGGGGGGGGGGAACCCCC...C.CCGCGGG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCt                                     <  2:3011927/92‑1 (MQ=2)                                aaTACGCCCTTCTTTCTTTTGGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGcc                                 <  2:1669152/101‑1 (MQ=18)                                 aTACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCt                                <  2:868409/101‑1 (MQ=21)                                    cGCTCTTCTTTCTTTGCGGGGGGGCGGGGGGGGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTgcg                             <  2:805220/101‑1 (MQ=17)                                       ccTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTgcgcgc                          <  2:5079872/101‑1 (MQ=21)                                            tttctttTCGGGGGGGCGGGGGGGGGAGCCCC...C.CCCG.GG...G...GG....CCCCCGGCGTGCCCCAGGCGGAAACGTCCCCCCCCGGAAACCCTGCCCCCGCGCGCCGc                     >  2:4423967/1‑101 (MQ=255)                                               tttttGGGGGGGGGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                  <  2:5312555/100‑1 (MQ=18)                                                ttttCGGGGGGGGGGGGGGTGAAACCCC...C.CCGCGGG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                  <  2:1349675/101‑1 (MQ=21)                                                ttttCGGGGGGGCGGGGGGGGAAGCCCC...C.C....CC...C...GGGGGCCCCCGGGCTTGCCCCGGGGGAAAGCTTCCCCCCCGGAAACCCTGCCCGCcgcgcgcgccttc                  >  2:2409782/1‑94 (MQ=255)                                                tttgggggggggGGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc                 <  1:4642702/96‑1 (MQ=18)                                                    ggggggggCGGGGGGTGACCCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCtt             <  2:1427717/100‑1 (MQ=18)                                                     gggggggGGGGGGGGGAACCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCttt            <  2:5614901/101‑1 (MQ=18)                                                      ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  1:3473759/101‑1 (MQ=21)                                                      ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  2:5463259/101‑1 (MQ=21)                                                      ggggggCGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc           <  2:3769442/101‑1 (MQ=21)                                                       gggggGGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCg          <  2:4416929/101‑1 (MQ=21)                                                        ggggCGGGGGGGGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCgg         <  1:3502339/101‑1 (MQ=21)                                                          ggCGGGGGGTCAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc       <  2:2413746/101‑1 (MQ=21)                                                            cGGGGGGTGAAGCCCG...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTTCCTGCGCGCGTCGCTTTCCCTTTTGGCCCg     >  1:4881740/1‑101 (MQ=18)                                                            cGGGGGGTGAAGCCCCT..CGC....GTG..T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg     <  1:3548126/101‑1 (MQ=25)                                                            cGGGGGGTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg     >  2:2458531/1‑101 (MQ=21)                                                             ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGGCTCGCTTTCCCTTTCGGCCCgg    >  2:296370/1‑101 (MQ=18)                                                             ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccccg    >  1:964044/1‑99 (MQ=21)                                                             ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg    >  2:3760503/1‑101 (MQ=21)                                                             ggggggTGAAGCCCC...C.CGCG.GG...T...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCgg    >  2:3026747/1‑101 (MQ=21)                                                              gggggTGAAGCCCC...C.CGCG.GG...G...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGCCGCTTTCCCTTTCTGCCCGgc   >  2:2652351/1‑101 (MQ=21)                                                               ggggTGAAGCCCC...C.C....GCGGGG...CG....CCTCGGGCTTGCCCGAGGCGTAAGCTTCCCCCCGTGGAAACCTTGCCTGCGCGCGTCGCTTTCCCTTGCGGCCCGgcg  >  2:1681885/1‑101 (MQ=21)                                                                                           |                                                                                        AACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAGCCCC...C.C....GT...C...CG....CCTCGGGCTTGCCCGAGGCGAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGCG  >  NC_014034/1732790‑1732949 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 20 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG