Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,889 T→G 1.8% intergenic (‑142/+51) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8890T→G 1.8% 3.8 ‑4.2 ‑0.1273intergenic (‑142/+51)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (113/155):  total (118/155)
Fisher's exact test strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.91e-01

Only 100 of 299 total aligned reads displayed.

CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989
                                                                                                       |                                                                                                        
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.Tg                                                                                                         <  1:3339634/101‑1 (MQ=255)
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.Tg                                                                                                         <  1:2015646/101‑1 (MQ=255)
    ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.gg                                                                                                     <  2:364974/101‑1 (MQ=255)
     cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGc                                                                                                    >  2:3405316/1‑101 (MQ=255)
      aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGcc                                                                                                   >  2:1737448/1‑101 (MQ=255)
       aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCt                                                                                                  <  1:2574411/101‑1 (MQ=255)
       aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCt                                                                                                  >  1:5047340/1‑101 (MQ=255)
       aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGACt                                                                                                  >  2:2599408/1‑101 (MQ=255)
        aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTTT.TGCAAGAAGAAACTTGC.GGctt                                                                                                  >  2:1086292/1‑99 (MQ=17)
        aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCtt                                                                                                 <  1:5298167/101‑1 (MQ=255)
        aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCtt                                                                                                 >  2:4340228/1‑101 (MQ=255)
        aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCtt                                                                                                 <  1:1029121/101‑1 (MQ=255)
           cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCAt                                                                                              >  1:1895732/1‑101 (MQ=255)
            tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATc                                                                                             <  2:842321/101‑1 (MQ=255)
             gACGCTGCGTCAGCCGGCGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCt                                                                                            <  2:3953971/101‑1 (MQ=255)
             gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGACGCTTC.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCt                                                                                            <  1:4985542/101‑1 (MQ=18)
              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCtt                                                                                           <  2:1484211/101‑1 (MQ=255)
                 cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGcc                                                                                        <  1:1138117/101‑1 (MQ=255)
                  tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCa                                                                                       <  1:2084756/101‑1 (MQ=255)
                  tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCa                                                                                       <  2:2663788/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGGAGGGGGGCGC.TGCCCCCCGTGCTGCGGGCTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCAt                                                                                      <  1:2320337/101‑1 (MQ=18)
                    cGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATa                                                                                     <  1:5005390/101‑1 (MQ=255)
                     gTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATaa                                                                                    <  1:3124619/101‑1 (MQ=255)
                     gTCAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATaa                                                                                    <  1:3429563/101‑1 (MQ=255)
                       cAGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAat                                                                                  >  2:5139625/1‑101 (MQ=255)
                        aGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAata                                                                                 >  2:1002499/1‑101 (MQ=255)
                        aGCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAata                                                                                 <  2:2999486/101‑1 (MQ=255)
                         gCCGACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAata.t                                                                               <  2:4751284/101‑1 (MQ=255)
                           cgtcgggccgggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.Tcc                                                                             <  2:3774836/98‑1 (MQ=255)
                           ccaccggccgggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.Tcc                                                                             <  2:4636348/96‑1 (MQ=255)
                            gACGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.Tccc                                                                            <  2:1754954/101‑1 (MQ=255)
                             aCGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.g                                                                          <  2:4279709/101‑1 (MQ=255)
                             aCGGGCCGGGCAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.g                                                                          <  1:3992487/101‑1 (MQ=255)
                                ggccgggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.gggg                                                                       <  2:2081259/101‑1 (MQ=255)
                                 tgacggacggggggCTC.TG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGC.GGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATA.TCCCAGGGGTg                                                                     >  1:2416509/9‑101 (MQ=2)
                                 gccgggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGt                                                                      <  2:2215207/101‑1 (MQ=255)
                                 gccgggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCCGGGTCTTTAT.CGCAAGATGGAAC.CGC.GGGCCTCATCTTTCCCCAAAAATTCCC.gggg                                                                       >  2:4890036/1‑101 (MQ=2)
                                    gggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCg                                                                   >  2:181206/1‑101 (MQ=255)
                                    gggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCg                                                                   <  1:2708885/101‑1 (MQ=255)
                                    gggcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCg                                                                   >  1:912455/1‑101 (MQ=255)
                                      gcAGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCggg                                                                 <  1:1553536/101‑1 (MQ=255)
                                       gaGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCgggg                                                                <  2:914900/100‑1 (MQ=255)
                                        aGGGGGGCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGc                                                               >  2:4046816/1‑101 (MQ=255)
                                         ggggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa                                                              <  1:3563679/101‑1 (MQ=255)
                                         ggggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa                                                              <  1:3409499/101‑1 (MQ=255)
                                         ggggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa                                                              <  2:656116/101‑1 (MQ=255)
                                           ggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCTGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa.ga                                                           <  1:4961305/101‑1 (MQ=255)
                                           ggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa.ga                                                           <  2:453711/101‑1 (MQ=255)
                                           ggggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa.ga                                                           <  1:440282/101‑1 (MQ=255)
                                            gggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCCTAAATA.TCCC.GGGGTGCGGGGCa.gag                                                          >  2:600081/1‑101 (MQ=255)
                                             ggCGC.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGc                                                         <  1:1692893/101‑1 (MQ=255)
                                               cgc.TGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGccc                                                       <  1:5619824/101‑1 (MQ=255)
                                                 gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGcccc                                                      <  2:1606945/96‑1 (MQ=255)
                                                      cccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCa                                                 <  1:1737547/101‑1 (MQ=255)
                                                      cccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCa                                                 >  2:4451061/1‑101 (MQ=255)
                                                      cccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCa                                                 >  2:4180449/1‑101 (MQ=255)
                                                        cccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAAc                                               <  1:1234628/101‑1 (MQ=255)
                                                        cccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAAc                                               >  2:2489224/1‑101 (MQ=255)
                                                          cGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCg                                             <  2:2756824/101‑1 (MQ=255)
                                                             ccTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAg                                          >  2:4995155/1‑101 (MQ=255)
                                                              cTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGa                                         >  2:1540280/1‑101 (MQ=255)
                                                               tGCGGACTCCCCCCGGGGTATTTTTGGGCAAGTGGAAAT.TG..GGGCTTTATTTTTCCATAAATA.TTCC.GGGGGGGGGGGGAGGGGCCCCGTCCAACCGAAGa                                         >  2:1998491/1‑101 (MQ=255)
                                                               tGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGAt                                        >  1:729265/1‑101 (MQ=255)
                                                                  ggACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGAc                                     <  2:3418987/101‑1 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACa                                    <  2:5757828/101‑1 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACa                                    >  1:2865827/1‑101 (MQ=255)
                                                                     cTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAg                                  <  1:4593060/101‑1 (MQ=255)
                                                                      tCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGa                                 >  1:2245370/1‑101 (MQ=255)
                                                                      tCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGa                                 >  1:87933/1‑101 (MQ=255)
                                                                       ccccccGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGAt                                <  2:3191575/101‑1 (MQ=255)
                                                                       ccccccGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGAt                                <  1:2075027/101‑1 (MQ=255)
                                                                        cccccGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGTA.GAGCCCCGCTCAACCGAAGATGACAAGATc                               <  1:5128256/101‑1 (MQ=255)
                                                                            cGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                           <  1:1373541/101‑1 (MQ=255)
                                                                              ggATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                         >  1:245226/1‑101 (MQ=255)
                                                                              ggATATTTAT.GGAAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                         <  1:5755939/101‑1 (MQ=255)
                                                                                 tatTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                      <  2:5344932/101‑1 (MQ=255)
                                                                                   tttAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGGA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    >  2:2287700/1‑101 (MQ=255)
                                                                                   tttAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    >  2:731538/1‑101 (MQ=255)
                                                                                   tttAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    <  1:764445/101‑1 (MQ=255)
                                                                                    ttAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                   >  1:3496040/1‑101 (MQ=255)
                                                                                     tAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  >  1:3045245/1‑101 (MQ=255)
                                                                                      aT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 <  1:4047063/101‑1 (MQ=255)
                                                                                      aT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 >  1:5385380/1‑101 (MQ=255)
                                                                                       t.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                <  2:1318391/101‑1 (MQ=255)
                                                                                         ggCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg               <  1:2824175/101‑1 (MQ=255)
                                                                                          gCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGGGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc              >  1:2619957/1‑101 (MQ=255)
                                                                                            aaGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg            >  2:1968597/1‑101 (MQ=255)
                                                                                             aGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga           >  1:5481331/1‑101 (MQ=255)
                                                                                              gATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat          <  1:4774919/101‑1 (MQ=255)
                                                                                                tGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGCTTTCGCtgatga        >  2:2569029/1‑101 (MQ=255)
                                                                                                 gAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg       >  2:2825914/1‑101 (MQ=255)
                                                                                                 gAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg       >  2:2446255/1‑101 (MQ=255)
                                                                                                 gAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg       >  1:3815844/1‑101 (MQ=255)
                                                                                                  aaaC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc      >  2:3279083/1‑101 (MQ=255)
                                                                                                    aC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  2:549752/101‑1 (MQ=255)
                                                                                                    aC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  2:3462305/101‑1 (MQ=255)
                                                                                                     c.TGC.TGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg   >  1:2762125/1‑101 (MQ=255)
                                                                                                     c.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg   <  1:2163036/101‑1 (MQ=255)
                                                                                                       tGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCTGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc  >  1:4536551/1‑101 (MQ=255)
                                                                                                       tGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc  <  2:4369905/101‑1 (MQ=255)
                                                                                                       |                                                                                                        
CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAAC.TGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG