BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,734,907	A→C	1.8%	intergenic (‑160/+33)	RCAP_rcc01603 ← / ← RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,734,907	0	A→C	1.8%	4.7 ‑4.5 0.0	281	intergenic (‑160/+33)	RCAP_rcc01603/RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (6/0): ref base (127/148): total (133/148)
Fisher's exact test strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.


TGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGT  >  NC_014034/1734808‑1735007


 gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCAt                                                                                                      <  1:3999672/101‑1 (MQ=255)


  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATa                                                                                                     <  1:5005390/101‑1 (MQ=255)


   gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaa                                                                                                    <  1:3429563/101‑1 (MQ=255)


   gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaa                                                                                                    <  1:3124619/101‑1 (MQ=255)


     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAat                                                                                                  <  2:2242265/101‑1 (MQ=255)


     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAat                                                                                                  <  2:2763945/101‑1 (MQ=255)


      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAata                                                                                                 >  2:1002499/1‑101 (MQ=255)


       gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAata.t                                                                                               <  2:4751284/101‑1 (MQ=255)


         cgtcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.Tcc                                                                                             <  2:3774836/98‑1 (MQ=255)


         ccaccggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.Tcc                                                                                             <  2:4636348/96‑1 (MQ=255)


         cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.Tcc                                                                                             <  2:3220933/101‑1 (MQ=255)


          gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.Tccc                                                                                            <  2:1754954/101‑1 (MQ=255)


          gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTACGGCCTTCATCTTGCCCTCAatacatc                                                                                             >  2:1716694/1‑97 (MQ=14)


           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCg                                                                                           <  2:3562984/101‑1 (MQ=255)


           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCg                                                                                           <  1:1698104/101‑1 (MQ=255)


           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCg                                                                                           <  2:4279709/101‑1 (MQ=255)


            cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCgg                                                                                          <  2:4502215/101‑1 (MQ=255)


               gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTCTTTATCGCAAGATGGAACCGCGGGCCTCATCTTTCCCCAAAAATTCCCgggg                                                                                        >  2:4890036/1‑101 (MQ=2)


                ccgggcAGGGGGTCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTg                                                                                      <  2:1063685/101‑1 (MQ=255)


                  gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCg                                                                                    <  1:1220977/101‑1 (MQ=255)


                  gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCg                                                                                    <  1:2708885/101‑1 (MQ=255)


                  gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCg                                                                                    >  2:181206/1‑101 (MQ=255)


                  gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCg                                                                                    >  1:912455/1‑101 (MQ=255)


                   ggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAACATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCGGGGGTGCgg                                                                                   <  1:2825189/101‑1 (MQ=17)


                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGc                                                                                >  1:5601957/1‑101 (MQ=255)


                         ggggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCaga                                                                             <  2:3409900/101‑1 (MQ=255)


                         ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCaga                                                                             <  1:2915534/101‑1 (MQ=255)


                         ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCaga                                                                             >  1:2653935/1‑101 (MQ=255)


                              cctgacgtgcGTCCTGCTGAAGCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGcccc                                                                        <  2:3317340/92‑1 (MQ=21)


                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCg                                                                       >  1:510128/5‑101 (MQ=255)


                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCt                                                                     <  1:2016460/99‑1 (MQ=255)


                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTc                                                                    <  1:3405521/100‑1 (MQ=255)


                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTc                                                                    >  1:2587863/2‑101 (MQ=255)


                                       cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                               <  1:5209605/101‑1 (MQ=255)


                                       cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                               >  1:249968/1‑101 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                              <  2:1292677/101‑1 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                              <  2:5045883/101‑1 (MQ=255)


                                          ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                                            >  2:4995155/1‑101 (MQ=255)


                                           cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                           >  2:5783672/1‑101 (MQ=255)


                                           cTGCGGACTCCCCCCGGCATATTTATGGCAAGATGAAACTGCGGCCTTTATCTTGCCATAAATA.TCGCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                           <  1:2425870/101‑1 (MQ=255)


                                            tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                                          <  2:2587721/101‑1 (MQ=255)


                                            tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                                          >  1:729265/1‑101 (MQ=255)


                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCGCCGCTCAACCGAACATg                                                         >  2:39702/1‑101 (MQ=255)


                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                                         <  1:2146076/101‑1 (MQ=255)


                                              cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGGCTTCATTTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                        >  1:4766690/1‑101 (MQ=255)


                                              cGGACCCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                        <  1:2215763/101‑1 (MQ=255)


                                               ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                       >  1:2372214/1‑101 (MQ=255)


                                                   tCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCCTCTTGCCATAAATA.TCCCGGGGCGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                                   >  2:937120/1‑101 (MQ=255)


                                                   tCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                                   >  1:87933/1‑101 (MQ=255)


                                                    ccccccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                  >  2:2640890/1‑101 (MQ=255)


                                                    ccccccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                  <  2:3191575/101‑1 (MQ=255)


                                                    ccccccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                  >  1:1656342/1‑101 (MQ=255)


                                                     cccccGGGATATTTATGGCAAGATGAAACTGCGTCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                 <  2:4402517/101‑1 (MQ=255)


                                                     cccccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                 <  1:3803851/101‑1 (MQ=255)


                                                       cccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                               <  2:518194/101‑1 (MQ=255)


                                                         cGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                             <  1:1373541/101‑1 (MQ=255)


                                                         cGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                             >  2:1542692/1‑101 (MQ=255)


                                                           ggATATTTATGGAAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                                           <  1:5755939/101‑1 (MQ=255)


                                                              tttttaggaaaaGATGAAACTGCGGCTTTCATTTTGCATTAATTC.CCCCGGGGTGCGGGGCAGCCCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                        <  2:2153898/91‑1 (MQ=25)


                                                              tatTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                        >  2:2671399/1‑101 (MQ=255)


                                                              tatTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                        <  2:5344932/101‑1 (MQ=255)


                                                              tatTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                        >  2:620266/1‑101 (MQ=255)


                                                               atTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                                       >  2:907518/1‑101 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                      >  2:689346/1‑101 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                      >  2:731538/1‑101 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                      <  1:764445/101‑1 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                      <  1:4549970/101‑1 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGAGGGGCAGAGACCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                      >  2:2936629/1‑101 (MQ=255)


                                                                 ttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                     <  1:4890317/101‑1 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                    <  1:2265793/101‑1 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                    >  1:3045245/1‑101 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                    >  2:4340472/1‑101 (MQ=255)


                                                                   aTGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                   >  1:5385380/1‑101 (MQ=255)


                                                                    tGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                  >  1:3841626/1‑101 (MQ=255)


                                                                        aaGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                              >  2:1968597/1‑101 (MQ=255)


                                                                        aaGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                              >  2:4120407/1‑101 (MQ=255)


                                                                          gATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                            >  1:351557/1‑101 (MQ=255)


                                                                           aggAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                           <  1:2081692/99‑1 (MQ=255)


                                                                             gAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                         >  1:441181/1‑101 (MQ=255)


                                                                             gAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                         >  2:2446255/1‑101 (MQ=255)


                                                                              aaaCTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                        <  2:2528155/101‑1 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                      >  2:1494744/1‑101 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                      <  2:549752/101‑1 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATA.TCCCGGAGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                      <  1:4983627/101‑1 (MQ=255)


                                                                                    cGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcgc                  >  1:3611790/1‑101 (MQ=255)


                                                                                     ggCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                 >  1:1742766/1‑101 (MQ=255)


                                                                                      gCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                >  1:2789259/1‑101 (MQ=255)


                                                                                      gCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                <  2:56868/101‑1 (MQ=255)


                                                                                       ccTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg               <  1:4076674/101‑1 (MQ=255)


                                                                                        cTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt              >  1:3420467/1‑101 (MQ=255)


                                                                                         ttCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt             >  1:5687306/1‑101 (MQ=255)


                                                                                          tCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc            >  1:3223400/1‑101 (MQ=255)


                                                                                           cATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg           >  2:3663232/1‑101 (MQ=255)


                                                                                           cATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg           <  1:5482107/101‑1 (MQ=255)


                                                                                               ttGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAg       >  1:4358960/1‑101 (MQ=255)


                                                                                                tGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg      >  2:2328687/1‑101 (MQ=255)


                                                                                                tGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg      >  2:2038058/1‑101 (MQ=255)


                                                                                                  ccATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc    >  2:3443258/1‑101 (MQ=255)


                                                                                                  ccATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc    <  1:101033/101‑1 (MQ=255)


                                                                                                    aTAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGt  >  1:4683388/1‑101 (MQ=255)


TGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGT  >  NC_014034/1734808‑1735007

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG