Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,908 T→G 1.8% intergenic (‑161/+32) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,9080T→G 1.8% 2.3 ‑3.7 ‑0.0279intergenic (‑161/+32)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (129/145):  total (134/145)
Fisher's exact test strand distribution p-value = 2.46e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 296 total aligned reads displayed.

GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008
                                                                                                      |                                                                                                       
gCGTCAGCCGACGGGCCGGGGAGGGGGGCGCTGCCCCCCGTGCTGCGGGCTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                         <  1:2320337/101‑1 (MQ=18)
gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                         <  1:3999672/101‑1 (MQ=255)
  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                                       <  1:2715708/101‑1 (MQ=255)
     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                                    <  2:2999486/101‑1 (MQ=255)
     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                                    >  1:2681179/1‑101 (MQ=255)
      gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata.t                                                                                                  <  2:4751284/101‑1 (MQ=255)
        cgtcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.Tcc                                                                                                <  2:3774836/98‑1 (MQ=255)
        ccaccggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.Tcc                                                                                                <  2:4636348/96‑1 (MQ=255)
          aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.g                                                                                             <  1:1698104/101‑1 (MQ=255)
          aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.g                                                                                             <  2:4279709/101‑1 (MQ=255)
          aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.g                                                                                             <  2:3562984/101‑1 (MQ=255)
           cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.gg                                                                                            <  2:4502215/101‑1 (MQ=255)
           agggccgggaAGGAGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATTTTGCCATAAATA.TCCC.gg                                                                                            <  2:2428561/100‑1 (MQ=14)
             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.gggg                                                                                          <  2:2081259/101‑1 (MQ=255)
              tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGC.GGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATA.TCCCAGGGGTg                                                                                        >  1:2416509/9‑101 (MQ=2)
              gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTCTTTAT.CGCAAGATGGAACCGC.GGGCCTCATCTTTCCCCAAAAATTCCC.gggg                                                                                          >  2:4890036/1‑101 (MQ=2)
              gccgggcAGGGGGGCGCTGCCCCCCGTCCTGAGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGt                                                                                         >  1:5125379/1‑101 (MQ=255)
                acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGC.GGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATA.TCCCAGGGGTgaa                                                                                      >  1:4564534/7‑99 (MQ=2)
                acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGC.GGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATA.TCCCAGGGGTgaa                                                                                      >  1:5474530/7‑99 (MQ=2)
                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCg                                                                                      <  1:1220977/101‑1 (MQ=255)
                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCg                                                                                      >  2:2736620/1‑101 (MQ=255)
                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa                                                                                 <  1:3409499/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCTGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa.ga                                                                              <  1:4961305/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCa.ga                                                                              >  2:1373453/1‑101 (MQ=255)
                        ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TACC.GCGCTGCGGGGCa.ga                                                                              >  2:1750500/1‑101 (MQ=17)
                            cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGccc                                                                          <  1:5619824/101‑1 (MQ=255)
                            cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGccc                                                                          >  1:251495/1‑101 (MQ=255)
                            cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGccc                                                                          <  1:880461/101‑1 (MQ=255)
                             gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGcccc                                                                         <  2:1606945/96‑1 (MQ=255)
                             gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGcccc                                                                         >  2:2218998/6‑101 (MQ=255)
                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCt                                                                      >  2:3365251/3‑101 (MQ=255)
                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCt                                                                      <  1:2016460/99‑1 (MQ=255)
                                    cccGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAAc                                                                  >  2:2489224/1‑101 (MQ=255)
                                       gTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGa                                                               <  2:5045883/101‑1 (MQ=255)
                                         ccTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAg                                                             >  2:4995155/1‑101 (MQ=255)
                                          cTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGa                                                            >  2:1540280/1‑101 (MQ=255)
                                          cTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGa                                                            >  2:5783672/1‑101 (MQ=255)
                                           tGCGGACTCCCCCCGGGGTATTTTTGGGCAAGTGGAAATTG..GGGCTTTATTTTTCCATAAATA.TTCC.GGGGGGGGGGGGAGGGGCCCCGTCCAACCGAAGa                                                            >  2:1998491/1‑101 (MQ=255)
                                            gCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCGCCGCTCAACCGAACATg                                                          >  2:39702/1‑101 (MQ=255)
                                              ggACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGAc                                                        <  2:3418987/101‑1 (MQ=255)
                                                 cTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAg                                                     >  2:1787649/1‑101 (MQ=255)
                                                 cTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAg                                                     <  1:3092644/101‑1 (MQ=255)
                                                  tCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCCTCTTGCCATAAATA.TCCC.GGGGCGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGa                                                    >  2:937120/1‑101 (MQ=255)
                                                  tCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGa                                                    <  1:2858282/101‑1 (MQ=255)
                                                   ccccccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGAt                                                   >  2:2640890/1‑101 (MQ=255)
                                                   ccccccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGAt                                                   >  1:1656342/1‑101 (MQ=255)
                                                    cccccGGGATATTTAT.GGCAAGATGAAACTGC.GTCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  2:4402517/101‑1 (MQ=255)
                                                    cccccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  1:3803851/101‑1 (MQ=255)
                                                    cccccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  2:1176842/101‑1 (MQ=255)
                                                     ccccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATcc                                                 <  2:1895623/101‑1 (MQ=255)
                                                      cccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCcg                                                <  2:518194/101‑1 (MQ=255)
                                                      cccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCcg                                                >  1:2811242/1‑101 (MQ=255)
                                                       ccGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               >  1:740882/1‑101 (MQ=255)
                                                        cGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                              <  1:1373541/101‑1 (MQ=255)
                                                          ggATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                                            >  1:245226/1‑101 (MQ=255)
                                                           gATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  1:2154020/1‑101 (MQ=255)
                                                             tatTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         <  2:5344932/101‑1 (MQ=255)
                                                             tatTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         >  2:620266/1‑101 (MQ=255)
                                                             tatTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         >  1:5006912/1‑101 (MQ=255)
                                                              atTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                                        >  2:907518/1‑101 (MQ=255)
                                                              atTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                                        >  1:5299994/1‑101 (MQ=255)
                                                               tttAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGGA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  2:2287700/1‑101 (MQ=255)
                                                               tttAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  2:689346/1‑101 (MQ=255)
                                                               tttAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  2:731538/1‑101 (MQ=255)
                                                                 tAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     >  2:4340472/1‑101 (MQ=255)
                                                                   t.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                   >  1:3841626/1‑101 (MQ=255)
                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                               >  2:4120407/1‑101 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             >  1:351557/1‑101 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             <  1:4774919/101‑1 (MQ=255)
                                                                           aggAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            <  1:2081692/99‑1 (MQ=255)
                                                                             gAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                          >  1:441181/1‑101 (MQ=255)
                                                                              nnaNNNN.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAgn                         <  1:640111/99‑2 (MQ=255)
                                                                              aaaCTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                         >  2:3279083/1‑101 (MQ=255)
                                                                               aaCTGC.GGGCTTCACCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  1:4995436/101‑1 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        >  1:991513/1‑101 (MQ=255)
                                                                                aCTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  2:549752/101‑1 (MQ=255)
                                                                                aCTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  2:3462305/101‑1 (MQ=255)
                                                                                 cTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg                      <  1:3451513/101‑1 (MQ=255)
                                                                                 aggcgggCCTTCTTCTTGCAATAAATA.GCGC.GGGGGGGGGGCCA.GAGCGCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  1:2936796/96‑1 (MQ=255)
                                                                                   gC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg                    <  1:5494687/101‑1 (MQ=255)
                                                                                    c.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcgc                   >  1:3611790/1‑101 (MQ=255)
                                                                                      ggCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCGAGCGcc                  >  2:2814257/1‑101 (MQ=255)
                                                                                      ggCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                  >  1:3198480/1‑101 (MQ=255)
                                                                                      ggCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                  >  1:1742766/1‑101 (MQ=255)
                                                                                      ggCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                  >  1:789696/1‑101 (MQ=255)
                                                                                       gCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                 <  2:56868/101‑1 (MQ=255)
                                                                                        ccTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                <  1:4076674/101‑1 (MQ=255)
                                                                                          ttCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  1:2599550/101‑1 (MQ=255)
                                                                                          ttCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  1:2219120/101‑1 (MQ=255)
                                                                                            cATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg            >  2:3663232/1‑101 (MQ=255)
                                                                                             aTCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           <  1:5711955/101‑1 (MQ=255)
                                                                                             aTCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           >  2:1042655/1‑101 (MQ=255)
                                                                                               cTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa         <  2:3198291/101‑1 (MQ=255)
                                                                                                ttGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAg        <  1:3421916/101‑1 (MQ=255)
                                                                                                ttGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAg        >  1:4358960/1‑101 (MQ=255)
                                                                                                 tGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  2:2579460/1‑101 (MQ=255)
                                                                                                  gCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt      >  1:3223917/1‑101 (MQ=255)
                                                                                                  gCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt      >  1:4697000/1‑101 (MQ=255)
                                                                                                   ccATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc     <  1:101033/101‑1 (MQ=255)
                                                                                                      tAAAAA.GCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt  <  1:937196/101‑1 (MQ=255)
                                                                                                      |                                                                                                       
GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTAT.GGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATA.TCCC.GGGGTGCGGGGCA.GAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG