Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,770,645 G→C 100% R326P (CGG→CCG)  lpxB → lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,770,6450G→C100.0% 879.1 231R326P (CGG→CCG) lpxBlipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  new base (114/117):  ref base (0/0):  total (114/117)

Only 100 of 242 total aligned reads displayed.

GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770550‑1770741
                                                                                               |                                                                                                
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCt                                                                                             >  1:3764536/1‑101 (MQ=255)
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCt                                                                                             <  1:694487/101‑1 (MQ=255)
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCt                                                                                             <  2:2403923/101‑1 (MQ=255)
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCt                                                                                             <  2:1210896/101‑1 (MQ=255)
 aCGTGGCATATCACGAAGCGCGCGATGCTGACCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTg                                                                                            <  1:1709342/101‑1 (MQ=255)
  cGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGt                                                                                           <  1:397025/101‑1 (MQ=255)
   gTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTc                                                                                          >  2:4061819/1‑101 (MQ=255)
   gTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTc                                                                                          <  1:893827/101‑1 (MQ=255)
    tGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCg                                                                                         <  1:3222306/101‑1 (MQ=255)
       cATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGcc                                                                                      >  2:4871034/1‑101 (MQ=255)
         tatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGa                                                                                    <  2:3140178/101‑1 (MQ=255)
            cACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAc                                                                                 >  1:2475786/1‑101 (MQ=255)
              cGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCg                                                                               >  1:235756/1‑101 (MQ=255)
               gAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGa                                                                              <  2:4364480/101‑1 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGac                                                                             >  2:1526851/1‑100 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             >  2:1025073/1‑101 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             <  2:2658282/101‑1 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             <  1:4202698/101‑1 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             >  2:949151/1‑101 (MQ=255)
                  gctcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATgg                                                                           <  2:3902381/98‑1 (MQ=255)
                  gcgcgcGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATgg                                                                           <  2:4719468/101‑1 (MQ=255)
                    gcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgcg                                                                         >  1:4845368/1‑101 (MQ=255)
                    gcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgcg                                                                         <  1:1192976/101‑1 (MQ=255)
                     cgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgcgc                                                                        <  1:3330087/101‑1 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                       <  1:20817/101‑1 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                       >  1:3513000/1‑101 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                       <  1:638825/101‑1 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                       <  2:2535296/101‑1 (MQ=255)
                       cgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCgg                                                                      >  1:2233327/1‑101 (MQ=255)
                        gATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCggg                                                                     >  2:3599287/1‑101 (MQ=255)
                         aTGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgc                                                                    <  2:5445823/101‑1 (MQ=255)
                         aTGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgc                                                                    <  2:5332215/101‑1 (MQ=255)
                          tGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcg                                                                   >  1:815971/1‑101 (MQ=255)
                           gCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcgc                                                                  >  2:2279258/1‑101 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 >  2:2844058/1‑101 (MQ=255)
                             tGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTg                                                                >  1:5509512/1‑101 (MQ=255)
                             tGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGACCGCGCGGGCGCTg                                                                >  2:4023201/1‑101 (MQ=255)
                              gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGc                                                               >  1:4766380/1‑101 (MQ=255)
                                tCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAg                                                             <  2:3411990/101‑1 (MQ=255)
                                 cGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAgg                                                            >  1:1611709/1‑101 (MQ=255)
                                  gACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGa                                                           >  2:1843353/1‑101 (MQ=255)
                                       ggTGACGCTGGTCAATCTGCTGTCTGAGCCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                      <  1:3256312/101‑1 (MQ=255)
                                        gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCTAAACCGATGGCGCGGGCGCTGCAGGATCTGtt                                                     <  2:4989081/101‑1 (MQ=255)
                                        gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGtt                                                     <  2:2149295/101‑1 (MQ=255)
                                        gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGtt                                                     <  1:135351/101‑1 (MQ=255)
                                        gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCGCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGtt                                                     <  1:4089181/101‑1 (MQ=255)
                                         tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                    <  1:2798571/101‑1 (MQ=255)
                                            cGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCgg                                                 <  1:5678206/101‑1 (MQ=255)
                                            cGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCgg                                                 >  1:3730860/1‑101 (MQ=255)
                                             gCTTGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGCAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCggg                                                <  2:1341139/101‑1 (MQ=255)
                                              cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                               <  1:5035181/101‑1 (MQ=255)
                                              cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                               <  1:5177379/101‑1 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGGGCTGCAGGATCCGTTGCggggt                                              >  1:2899997/1‑99 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGAt                                              >  1:473640/1‑101 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGAt                                              <  2:2121849/101‑1 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGCTc                                             >  2:1430135/1‑101 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             >  1:3136050/1‑101 (MQ=255)
                                                 gTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGTGGCCGGGTCCGTTGCggggccc                                            >  2:231561/1‑97 (MQ=255)
                                                  tCAATCTGGTGTCTGGGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACGGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATccc                                           >  1:4883993/1‑100 (MQ=255)
                                                  tCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCg                                           <  1:1025148/101‑1 (MQ=255)
                                                  tCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCg                                           >  2:1624466/1‑101 (MQ=255)
                                                  tCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATACg                                           >  2:5799774/1‑101 (MQ=255)
                                                     aTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCaa                                        >  2:4493473/1‑101 (MQ=255)
                                                      tCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAc                                       <  1:4052519/101‑1 (MQ=255)
                                                       cTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcg                                      >  1:982031/1‑101 (MQ=255)
                                                         ggTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgcc                                    <  1:151641/101‑1 (MQ=255)
                                                          gtgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCCACGGCGCGGGCACCGCAGGACCTGTTGCTGGATTCACAAcgccg                                   >  2:896596/1‑101 (MQ=255)
                                                           tgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccgc                                  >  2:4205849/1‑101 (MQ=255)
                                                             tCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcgc                                >  1:1068830/1‑101 (MQ=255)
                                                               tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCCACGCcgcgccg                              >  2:1457509/1‑99 (MQ=255)
                                                               tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcgg                              >  1:1867587/1‑101 (MQ=255)
                                                               tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCCGGATCCGCAACGCCGCgcgg                              >  2:4542832/1‑101 (MQ=255)
                                                                 agaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcggcg                            >  1:4718783/1‑101 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                         <  2:2899830/101‑1 (MQ=255)
                                                                     ccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAgg                        <  1:2635380/101‑1 (MQ=255)
                                                                     ccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAgg                        >  2:5303278/1‑101 (MQ=255)
                                                                      cGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGt                       >  2:3807616/1‑101 (MQ=255)
                                                                       gggCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAgggt                      >  2:5667410/1‑99 (MQ=255)
                                                                         gCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTc                    <  2:69531/101‑1 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                  >  2:3245011/1‑101 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                  >  1:2106741/1‑101 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                  >  1:4336292/1‑101 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                  >  2:4739800/1‑101 (MQ=255)
                                                                                cGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATg             >  1:4647168/1‑101 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg            <  1:1933627/101‑1 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg            <  1:5059957/101‑1 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg            <  1:4543104/101‑1 (MQ=255)
                                                                                   attcccccggcccggCCTGTCGGCAAAAACCGTGGGCGCGGGCGCGGCAGGTTCTGTGGCGGGCCCCGCACCGCCGCGCGGCGCAGGTTCCCGCCATGGaa          <  2:518618/94‑1 (MQ=255)
                                                                                   aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          >  1:1421027/1‑101 (MQ=255)
                                                                                    aTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaaa         >  1:3061847/1‑101 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCCTGGAAac        >  2:3767696/1‑101 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAac        >  1:4385009/1‑101 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAac        <  1:5311327/101‑1 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGGTTCCGCCATGGAAac        >  2:2020712/1‑101 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg       <  1:4482336/101‑1 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg       <  2:3970103/101‑1 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg       >  2:3100670/1‑101 (MQ=255)
                                                                                         tCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgacg    <  1:3676517/101‑1 (MQ=255)
                                                                                           ggcccggcCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAt  >  1:5325813/1‑101 (MQ=255)
                                                                                           ggcccggcCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAt  <  1:5761535/101‑1 (MQ=255)
                                                                                               |                                                                                                
GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770550‑1770741

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG