Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,813,103	G→C	100%	intergenic (+147/‑71)	glnA3 → / → fabD	glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,813,103	0	G→C	100.0%	300.6	91	intergenic (+147/‑71)	glnA3/fabD	glutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (85/6):  ref base (0/0):  total (85/6)

CCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTC  >  NC_014034/1813006‑1813194                                                                                                    |                                                                                            ccGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGTCGCGGGGTCCCGGGGGTGCGTA.CGccc                                                                                          >  2:4677825/1‑101 (MQ=37)     ggTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggc                                                                                       >  1:2623725/1‑101 (MQ=37)      gTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcg                                                                                      >  1:3016794/1‑101 (MQ=37)      gTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcg                                                                                      >  1:2962093/1‑101 (MQ=37)        gcgactgcgactCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgct                                                                                    >  1:4682299/1‑99 (MQ=37)          gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                  >  2:1386230/1‑97 (MQ=25)           actgcgactCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                                >  1:583545/1‑101 (MQ=25)            ctgcgactCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCg                                                                               >  2:5777457/1‑101 (MQ=25)              gcgactCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGt                                                                             >  2:2332807/1‑101 (MQ=25)                 actCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGc                                                                          >  2:4097525/1‑101 (MQ=25)                 actCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGc                                                                          >  2:1850903/1‑101 (MQ=25)                 actCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGc                                                                          >  1:1763507/1‑101 (MQ=25)                  ctctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                         >  1:1456983/1‑101 (MQ=25)                  ctctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                         >  1:581734/1‑101 (MQ=25)                   tctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                        >  2:2996118/1‑101 (MQ=25)                   tctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                        >  1:2365739/1‑101 (MQ=25)                    ctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                       >  1:203437/1‑101 (MQ=25)                    ctTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                       >  1:2873724/1‑101 (MQ=25)                     tttGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                      >  1:2975350/1‑101 (MQ=25)                     tttGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                      >  2:5694075/1‑101 (MQ=25)                       tGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGa                                                                    >  2:1847190/1‑101 (MQ=25)                       tGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGa                                                                    >  2:2786620/1‑101 (MQ=25)                        gCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                                   >  2:4424859/1‑101 (MQ=25)                         cGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACg                                                                  >  2:438547/1‑101 (MQ=25)                         cGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACg                                                                  >  2:3857102/1‑101 (MQ=25)                           tATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                                >  1:5111208/1‑101 (MQ=25)                            aTTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                               >  1:204914/1‑101 (MQ=25)                            aTTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                               >  1:3271552/1‑101 (MQ=25)                            aTTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                               >  2:476294/1‑101 (MQ=25)                             tttGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                              >  2:3416165/1‑101 (MQ=25)                             tttGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                              >  1:3698636/1‑101 (MQ=25)                             tttGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                              >  2:4172312/1‑101 (MQ=25)                              ttGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCg                                                             >  2:5492539/1‑101 (MQ=25)                               tGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                                            >  2:5669293/1‑101 (MQ=25)                                 gaccAAAAAAAACCCCCGGCTTTTTTTTTTGGTGAAAACGCGCGGGGGGGGCCGGGGGGCGCGAC.CCCCCCGGCGCTTCCGGTTGCTCCGGACGCTGCGTc                                                           <  2:3701572/99‑1 (MQ=255)                                   ccAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAg                                                        >  2:269131/1‑101 (MQ=37)                                    cAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                       >  2:1473328/1‑101 (MQ=37)                                    cAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                       >  1:5762460/1‑101 (MQ=25)                                    cAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                       >  1:5370356/1‑101 (MQ=37)                                     aaGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                      >  1:456466/1‑101 (MQ=25)                                     aaGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                      >  2:1802211/1‑101 (MQ=25)                                      aGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                     >  1:3879505/1‑101 (MQ=25)                                      aGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                     >  2:3432658/1‑101 (MQ=25)                                       gAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGTGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTag                                                    >  1:2137136/1‑101 (MQ=37)                                       gAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTag                                                    >  1:2567903/1‑101 (MQ=25)                                         aaaaa.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTagag                                                  >  1:2210898/1‑101 (MQ=25)                                           aaa.CCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                                >  1:1931062/1‑101 (MQ=25)                                               cccGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                             >  2:4263918/1‑101 (MQ=37)                                                  ggCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                          >  1:1847369/1‑101 (MQ=37)                                                  ggCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                          >  2:2981094/1‑101 (MQ=25)                                                  ggCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                          >  2:1204313/1‑101 (MQ=25)                                                   gcgtTTTTTCTGGGTTCAAATCCGCCGGGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGa                                         <  1:2626841/101‑1 (MQ=25)                                                   gcgcTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGa                                         >  1:4639688/1‑101 (MQ=25)                                                   gcgcTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGa                                         >  1:1049460/1‑101 (MQ=25)                                                      cTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                      >  2:1717076/1‑101 (MQ=25)                                                      cTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                      >  1:459739/1‑101 (MQ=25)                                                      cTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                      >  1:2850036/1‑101 (MQ=25)                                                       tttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.TCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTg                                     >  1:1463888/1‑101 (MQ=25)                                                       tttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTg                                     >  2:13618/1‑101 (MQ=25)                                                       tttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTg                                     >  2:4590178/1‑101 (MQ=25)                                                        ttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGt                                    >  2:2667507/1‑101 (MQ=25)                                                        ttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGt                                    >  1:963471/1‑101 (MQ=25)                                                         tttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                   >  2:591554/1‑101 (MQ=25)                                                          ttCTTGGTTCAAATACGCCGCGGGGGTCTGGGGCCGCGAA.GACCCCGGCGCTTCCGGTTGCTCCGGACCCTGCGTCAAGATAGAGAAGGCCAGATTTGTCa                                  >  2:3529457/1‑101 (MQ=255)                                                          ttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                  >  2:763101/1‑101 (MQ=25)                                                          ttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                  >  1:4110/1‑101 (MQ=25)                                                            cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  1:5095375/1‑101 (MQ=25)                                                             ttGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg                               >  1:920966/1‑101 (MQ=25)                                                               ggTTCAAATCCGCGGGGGGGGCCGGGGGGCGCGAA.GCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAaggag                             <  2:3267844/101‑1 (MQ=255)                                                               ggTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAaggag                             >  1:4705624/1‑101 (MQ=25)                                                                gTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGATAAGGCCAGATTTGTCAaggagg                            >  1:5104715/1‑101 (MQ=25)                                                                 ttCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagatcgg                           >  1:3964167/1‑96 (MQ=25)                                                                   cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                         >  1:5467526/1‑101 (MQ=25)                                                                   cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                         >  2:4640776/1‑101 (MQ=25)                                                                    aaaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCggg                        >  1:235964/1‑101 (MQ=37)                                                                       cccgccgggggggCCGGGGGGCGCGAC.CCCCCCGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                     <  2:3550400/93‑1 (MQ=255)                                                                          gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                  >  1:2853399/1‑101 (MQ=37)                                                                           ccgggggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGc                 <  1:1674534/97‑1 (MQ=37)                                                                           ccgcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGc                 >  1:3124544/4‑101 (MQ=37)                                                                             gggggggTCCGGGGGCGCGAA.CCCCCCGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGt               <  2:3714838/99‑1 (MQ=38)                                                                             gcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGt               >  2:500711/2‑101 (MQ=37)                                                                             gcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGt               >  2:409710/2‑101 (MQ=37)                                                                              ggggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg              <  1:1674899/100‑1 (MQ=38)                                                                              cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg              >  2:3172753/1‑101 (MQ=38)                                                                              cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg              >  1:2432271/1‑101 (MQ=38)                                                                               gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGaagatcg             >  2:3134456/1‑95 (MQ=37)                                                                               gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  1:4792629/1‑101 (MQ=38)                                                                               gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  2:5324842/1‑101 (MQ=38)                                                                                   gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg         >  2:1774070/1‑101 (MQ=38)                                                                                    tCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGGTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATgg        >  1:5129122/1‑101 (MQ=255)                                                                                      cgggggCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgt      >  1:1628521/1‑101 (MQ=255)                                                                                       gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg     >  2:3442112/4‑101 (MQ=255)                                                                                       gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg     >  2:2386194/4‑101 (MQ=255)                                                                                         gggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGGGtt   >  2:2579883/2‑101 (MQ=255)                                                                                         gggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCCGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGtt   >  1:2986100/2‑101 (MQ=255)                                                                                          ggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTc  <  2:2137014/101‑1 (MQ=255)                                                                                                    |                                                                                            CCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAA.CCCGGCGCTTTTTCTTGGTTCAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTC  >  NC_014034/1813006‑1813194 Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG