Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,951,208 G→C 92.8% A129G (GCG→GGG)  ssb1 ← single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,951,2080G→C92.8% 24.6 ‑4.2 0.0168A129G (GCG→GGG) ssb1single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  new base (86/68):  ref base (11/1):  total (99/69)
Fisher's exact test strand distribution p-value = 1.54e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCG  >  NC_014034/1951115‑1951298
                                                                                               |                                                                                           
 ccGACGAGGGGGGCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTg                                                                                    >  1:3132392/1‑101 (MQ=255)
 ccGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAAttc                                                                                    >  2:1403304/1‑100 (MQ=255)
  cgacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGc                                                                                   >  2:1986877/1‑101 (MQ=255)
  cgacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGc                                                                                   >  2:969447/1‑101 (MQ=255)
   gacggGGGGGCCGGGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGcc                                                                                  <  1:1285632/101‑1 (MQ=255)
   gacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCGACGC..CGTAATTGca                                                                                  >  1:2676497/1‑100 (MQ=255)
    gggggggggCGGAGGGCCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCa                                                                                 <  1:1992049/97‑1 (MQ=255)
    aggggggggCCGAGGGGCCGCCCCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCa                                                                                 <  1:1094366/97‑1 (MQ=255)
     ggggggggCGGAGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAc                                                                                <  1:4877870/98‑1 (MQ=255)
      gtggaggCGGAGGTGCCCCGGGCCCCGCCGCAATAGCCCGGGCCGTCACCGTGGCCGCCGCCATA.GCCGCCGCCCTGCCAACCG.CCGC.CGTAATTGCCAcc                                                                               <  2:2733938/96‑1 (MQ=255)
      gggggggCCGGGGCGCCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAcc                                                                               <  2:4434586/99‑1 (MQ=255)
      gaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAcc                                                                               >  1:4071096/1‑101 (MQ=255)
       aGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAccg                                                                              >  2:1832077/1‑101 (MQ=255)
       aGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGCAATTGCCAccg                                                                              >  2:648311/1‑101 (MQ=255)
         ggggCGGAGGACCCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCAccgcc                                                                            <  2:1563960/101‑1 (MQ=255)
         ggggCAGGGGCGCCGCCCCCCCCGCCCCCATGGCCCGGGCCGTCCCCGGAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAccgcc                                                                            <  1:3590629/101‑1 (MQ=255)
          gggCCGGGGGCCCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAccgccg                                                                           <  2:2790188/101‑1 (MQ=255)
             ccGGGGCGCCCCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACcgccgccc                                                                        <  2:5766495/101‑3 (MQ=255)
              gggggcgcccccgccCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCcgcccg                                                                       <  2:367006/95‑1 (MQ=255)
              cGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCcgcccg                                                                       >  1:1929559/1‑101 (MQ=255)
              cGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCcgcccg                                                                       >  1:275392/1‑101 (MQ=255)
              cGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCcgcccg                                                                       >  1:3044815/1‑101 (MQ=255)
              cGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCcgcccg                                                                       >  1:3080149/1‑101 (MQ=255)
                 ggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgc                                                                    >  2:2848395/1‑101 (MQ=255)
                  gagCCGCCGCCCCCGCCGCCATACCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgcc                                                                   <  2:1020931/101‑1 (MQ=255)
                  gagCCGCCGCCACCGCTGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgcc                                                                   >  1:1794585/1‑101 (MQ=255)
                      cgccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccg                                                               >  2:4968299/1‑101 (MQ=255)
                       gccgcccccgcccccaAAGCCCGGCCCGTCTCCGTAGCCCCCGCCATA.GCCCCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccgc                                                              <  2:1338848/101‑1 (MQ=255)
                       gccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccgc                                                              >  2:4243821/1‑101 (MQ=255)
                        cccccccccccgccaTAGCCCGGGCGGTCATCGTAGCCCCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccgcc                                                             <  1:3445063/92‑1 (MQ=255)
                           ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGT.GGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                          >  1:4964793/1‑101 (MQ=255)
                           ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                          >  2:2498479/1‑101 (MQ=255)
                            ccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATcc                                                         <  1:2133692/99‑1 (MQ=255)
                            caccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATcc                                                         >  2:3360463/1‑101 (MQ=255)
                              ccgccgccaTAGCCGGGCCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                       <  1:4290454/101‑1 (MQ=255)
                              ccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACAGCCGCCCGAGCCGCCGCCATCCCg                                                       >  2:3005000/1‑101 (MQ=255)
                              ccgccgccaTACCCCGGGCCGTCATCGTAGCCGCCCCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                       <  1:2516622/101‑1 (MQ=255)
                               cgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCgg                                                      >  1:1460523/1‑101 (MQ=255)
                                ccccccaTCGCCCGGGCCTTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                     <  2:3227889/97‑1 (MQ=255)
                                  cgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                   >  1:1851961/1‑101 (MQ=255)
                                  cgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                   >  2:4507944/1‑101 (MQ=255)
                                  cgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                   >  2:3485674/1‑101 (MQ=255)
                                  ccccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                   <  1:4244081/99‑1 (MQ=255)
                                   gccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                  >  1:23160/1‑101 (MQ=255)
                                   gccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                  >  2:2348925/1‑101 (MQ=255)
                                     caTGGCCGGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                <  1:5354829/101‑1 (MQ=255)
                                     caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                >  2:845213/1‑101 (MQ=255)
                                     caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                >  1:1011114/1‑101 (MQ=255)
                                     caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                >  1:3401902/1‑101 (MQ=255)
                                      acgccccggcccGCCACCGTAGCCGCCGCCATA.GCCGCCCCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                               <  1:5412966/91‑1 (MQ=255)
                                      aTAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                               >  2:4620556/1‑101 (MQ=255)
                                       tAGCCCGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAag                                              >  2:4387863/1‑101 (MQ=255)
                                        aGCCGGGGCCGTCATCGAAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                             <  1:3751563/101‑1 (MQ=255)
                                           ccGGGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcagc                                          >  1:4075680/1‑101 (MQ=255)
                                             gggcCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                        <  1:776924/99‑1 (MQ=255)
                                              gnccGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                       >  2:2995263/3‑101 (MQ=255)
                                              ggcCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                       >  1:4876933/2‑101 (MQ=255)
                                               gcCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCa                                      >  2:3337505/1‑101 (MQ=255)
                                                ccGTCATCGTAGCAGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAg                                     >  1:4656580/1‑101 (MQ=255)
                                                     aTCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                >  1:2428241/1‑101 (MQ=255)
                                                       cGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                              >  2:697675/1‑101 (MQ=255)
                                                        gTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                             >  2:546827/1‑101 (MQ=255)
                                                        gTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCACGGCCGACAAGCAGCGCAAGGTTGCCCg                             >  2:1688966/1‑101 (MQ=255)
                                                        gTACCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                             <  2:19624/101‑1 (MQ=255)
                                                         tAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                            >  1:4606857/1‑101 (MQ=255)
                                                         tAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGACGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                            >  2:3141777/1‑101 (MQ=255)
                                                         tAGCCCCCG.CATAGGCCGCCTCCGGGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                            <  2:1731314/101‑1 (MQ=255)
                                                          aGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                           >  1:111577/1‑101 (MQ=255)
                                                           gccgccgccATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtat                          >  1:3534952/1‑101 (MQ=255)
                                                            cgcccgccATG.CCCCCCCCCCTGCCAACCG.CCGC.CGTAATTGCCCCCCCCGCCCGAGCCCCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtata                         <  2:1144491/98‑1 (MQ=255)
                                                            ccgccgccATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtata                         <  2:81350/101‑1 (MQ=255)
                                                             cgccgccATA.GCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAg                        <  2:5025521/101‑1 (MQ=255)
                                                             cgcccccATA.GCCGCCGCCCTGGAAACCG.CCGC.CGTAATTGCCGCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAg                        <  1:4448600/101‑1 (MQ=255)
                                                              cccgccATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                       <  2:1794486/100‑1 (MQ=255)
                                                                cgccATA.GCCCCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgggg                     <  1:1403392/101‑1 (MQ=255)
                                                                  ccATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcg                   >  1:938573/1‑101 (MQ=255)
                                                                  ccATA.GCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcg                   <  2:1213437/101‑1 (MQ=255)
                                                                   cATA.GCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                  >  1:3337893/1‑101 (MQ=255)
                                                                    aTA.GCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                 >  1:383375/1‑101 (MQ=255)
                                                                    aTA.GCCGCCCCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                 <  2:2358430/101‑1 (MQ=255)
                                                                     tcgcccgcccccCGCCGACCCG.CCGC.CGTAATTGCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                 <  1:5198178/97‑1 (MQ=255)
                                                                     tA.GCCGCCGCCCGGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcag                <  2:4075433/101‑1 (MQ=255)
                                                                        gccgccgccCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagca              <  1:613264/101‑1 (MQ=255)
                                                                        gcccccgccCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcggatgt              <  2:3112645/101‑6 (MQ=255)
                                                                         ccgcccccCTGCGAACCG.CCGC.CGTAATTGCCGCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcac             <  1:235280/101‑1 (MQ=255)
                                                                          cgccgccCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc            >  2:3344854/1‑101 (MQ=255)
                                                                          cgccgccCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc            >  1:1715210/1‑101 (MQ=255)
                                                                          cgccgccCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc            >  2:3483073/1‑101 (MQ=255)
                                                                          cgccgccCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc            >  2:3546400/1‑101 (MQ=255)
                                                                           gccgccCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacca           <  1:2892889/101‑1 (MQ=255)
                                                                             cgccCGGCGACCCG.CCGC.CGTAATTGCCGCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCaccacc         <  2:3455202/101‑1 (MQ=255)
                                                                               cccTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTc       <  1:4787623/101‑1 (MQ=255)
                                                                               cccTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTc       <  2:1810900/101‑1 (MQ=255)
                                                                                ccTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      >  2:2693467/1‑101 (MQ=255)
                                                                                ccTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      >  1:5443253/1‑101 (MQ=255)
                                                                                 cTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCGCGTATAGGGGCGCAGCACCACCTCgg     >  1:4860772/1‑101 (MQ=255)
                                                                                 cTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgc     >  2:2819029/1‑100 (MQ=255)
                                                                                  tGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgga    >  1:1489348/1‑100 (MQ=255)
                                                                                   gCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTc   >  1:858382/1‑101 (MQ=255)
                                                                                    cGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg  >  1:1438976/1‑101 (MQ=255)
                                                                                               |                                                                                           
CCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATA.GCCGCCGCCCTGCGAACCG.CGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCG  >  NC_014034/1951115‑1951298

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG