Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,555 C→G 46.6% R40R (CGC→CGG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5550C→G46.6% 0.0 ‑2.8 ‑1.3368R40R (CGC→CGG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (75/96):  ref base (106/90):  total (181/187)
Fisher's exact test strand distribution p-value = 5.96e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.27e-02

Only 100 of 413 total aligned reads displayed.

CGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCC  >  NC_014034/2155456‑2155655
                                                                                                   |                                                                                                    
cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa                                                                                                     <  2:4836274/101‑1 (MQ=255)
cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa                                                                                                     <  1:3159799/101‑1 (MQ=255)
  tAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGGATTCGACCGAGGTGCGGGCGCGCAAg                                                                                                   >  2:2410653/1‑101 (MQ=255)
       gtcatcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccg                                                                                              <  1:4629447/97‑1 (MQ=255)
         cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                            >  1:2225494/1‑101 (MQ=255)
          gtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCtt                                                                                           >  2:2917521/1‑101 (MQ=255)
          atcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggt                                                                                           >  1:651469/2‑98 (MQ=255)
            cgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttt                                                                                         >  1:2503920/1‑99 (MQ=255)
            cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                                                                                         >  1:769403/1‑101 (MQ=255)
             gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt                                                                                        <  1:2953231/101‑1 (MQ=255)
              aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggttttc                                                                                       >  1:3503305/1‑94 (MQ=255)
              aTGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGAGGCTGATGTGGCATTCGACCGCGGTGCGGGCGCGCAAGCGCCGCTTCCta                                                                                       >  1:1003410/1‑100 (MQ=255)
               tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGa                                                                                      <  1:4683646/101‑1 (MQ=255)
               tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGa                                                                                      <  2:4611432/101‑1 (MQ=255)
                  cATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGcc                                                                                   >  1:2012616/1‑101 (MQ=255)
                  cATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGcc                                                                                   >  2:3588615/1‑101 (MQ=255)
                   aTCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcg                                                                                  >  1:1390065/1‑101 (MQ=255)
                     cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTAGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg                                                                                >  2:5315010/1‑101 (MQ=255)
                      tGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGa                                                                               >  1:4144317/1‑101 (MQ=255)
                        gggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgc                                                                             <  1:1649241/101‑1 (MQ=255)
                         ggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgct                                                                            <  2:1389977/101‑1 (MQ=255)
                          gcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctg                                                                           <  1:155895/101‑1 (MQ=255)
                            ggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgct                                                                         <  1:1301092/101‑1 (MQ=255)
                              ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGctct                                                                       >  2:2355081/1‑101 (MQ=255)
                                  gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCCTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGa                                                                   >  1:3411325/1‑101 (MQ=255)
                                      cgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTg                                                               <  1:3747026/99‑1 (MQ=255)
                                      ccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTg                                                               >  2:610961/1‑101 (MQ=255)
                                       cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                              >  2:4494418/1‑101 (MQ=255)
                                        acGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCGCTGGGAGCTGcc                                                             >  2:2775843/1‑101 (MQ=255)
                                         cGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccg                                                            >  1:4360386/1‑100 (MQ=255)
                                          gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccgg                                                           >  1:990257/1‑99 (MQ=255)
                                           gtGGCGGCGGCGCTGGCCGGGGGCCTGATGGGGCCTTCGACCGAGGTGCGGGCGCGGCGGCGCGGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGt                                                          <  1:5267629/100‑1 (MQ=255)
                                           aTGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGt                                                          >  2:5700906/1‑101 (MQ=255)
                                             ggcggcggcGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTcc                                                        >  2:1988443/1‑100 (MQ=255)
                                                ggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTggccg                                                     <  1:5722712/101‑3 (MQ=255)
                                                 cgggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGt                                                    <  1:3942843/99‑1 (MQ=255)
                                                  cggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGtg                                                   <  1:54034/101‑2 (MQ=255)
                                                    gcgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGgg                                                 <  2:2177251/101‑1 (MQ=255)
                                                      gcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCa                                               <  1:2818347/101‑1 (MQ=255)
                                                        tggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATg                                             >  2:3791335/3‑101 (MQ=255)
                                                         ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                            >  2:1505514/2‑101 (MQ=255)
                                                         ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                            >  2:337241/2‑101 (MQ=255)
                                                          gCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGc                                           <  2:456088/101‑1 (MQ=255)
                                                           ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                          >  2:3013347/1‑101 (MQ=255)
                                                           ccGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCg                                          <  2:1778947/101‑1 (MQ=255)
                                                           ccGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCg                                          <  1:878523/101‑1 (MQ=255)
                                                             gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                        <  1:3032594/101‑1 (MQ=255)
                                                             gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                        <  2:3396925/101‑1 (MQ=255)
                                                              ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                       >  1:3293969/1‑101 (MQ=255)
                                                              ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                       >  1:4253216/1‑101 (MQ=255)
                                                              ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGACCTGCTGTGGGAGCTGCCGGTAGCCGTGGGCATGGCGatc                                       >  1:1145910/1‑101 (MQ=255)
                                                              ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatc                                       <  2:2586617/101‑1 (MQ=255)
                                                               gcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatca                                      <  1:5314182/101‑1 (MQ=255)
                                                                cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                     >  2:4191310/1‑101 (MQ=255)
                                                                cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                     <  2:4333517/101‑1 (MQ=255)
                                                                cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                     <  1:2115734/98‑1 (MQ=255)
                                                                 ggcTGATGTCGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGCCGGCATGGCGatcatc                                    >  2:1892353/1‑101 (MQ=255)
                                                                   cTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGGGGCCGTGGGCATGGCGATCATCgg                                  >  1:5624696/1‑101 (MQ=255)
                                                                    tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCNGCATGGCGATCATCGGc                                 >  2:1636639/1‑101 (MQ=255)
                                                                    tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                 >  2:5740650/1‑101 (MQ=255)
                                                                    tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                 >  2:479348/1‑101 (MQ=255)
                                                                     gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGGTTCATGAGCGGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                <  1:5697537/101‑1 (MQ=255)
                                                                     gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                >  1:1937200/1‑101 (MQ=255)
                                                                     gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                >  2:5222912/1‑101 (MQ=255)
                                                                     gATGTGGCATGCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                <  2:4088103/101‑1 (MQ=255)
                                                                      aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                               >  2:308831/1‑101 (MQ=255)
                                                                      aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                               >  1:5789244/1‑101 (MQ=255)
                                                                       tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                              <  2:5036511/101‑1 (MQ=255)
                                                                       tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                              >  1:233074/1‑101 (MQ=255)
                                                                       tggggCATTCGACCGAGGTACGTGCGCGCAGGCGCCGAGTCCTGAGCCGCGAGCTGCTCGGGTAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                              <  2:881808/98‑1 (MQ=255)
                                                                        gtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAgg                             >  2:2107208/1‑101 (MQ=255)
                                                                        gtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAgg                             <  2:1267989/101‑1 (MQ=255)
                                                                          tacATTCGGCCGAGCTGCGGGCGCAGCGGCGCCGGATTTTCGGGCGCCAACTGCTGTGGGAGGTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAgggg                           <  2:3267098/99‑3 (MQ=255)
                                                                           gCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCgg                          <  1:3791577/101‑1 (MQ=255)
                                                                           gCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCgg                          <  2:5538610/101‑1 (MQ=255)
                                                                            cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCggg                         >  1:1813411/1‑100 (MQ=255)
                                                                            cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                         <  1:5765030/101‑1 (MQ=255)
                                                                            cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                         <  1:3647835/101‑1 (MQ=255)
                                                                            cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                         <  2:4265192/101‑1 (MQ=255)
                                                                             aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                        >  1:3875897/1‑101 (MQ=255)
                                                                             aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                        <  1:81433/101‑1 (MQ=255)
                                                                              ttCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTgg                       <  2:3174381/101‑1 (MQ=255)
                                                                                 gACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcgg                    <  2:1149417/101‑1 (MQ=255)
                                                                                  aCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcggc                   <  1:5583279/101‑1 (MQ=255)
                                                                                    cGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                 <  2:2692236/101‑1 (MQ=255)
                                                                                     gAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                <  1:5057151/101‑1 (MQ=255)
                                                                                     gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                >  1:5141459/1‑101 (MQ=255)
                                                                                      aGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGGGGCGGCtttt               >  1:1212567/1‑99 (MQ=255)
                                                                                      aGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt               >  2:3516204/1‑101 (MQ=255)
                                                                                        gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt             <  1:4536868/101‑1 (MQ=255)
                                                                                         tGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTc            <  1:4894211/101‑1 (MQ=255)
                                                                                           cGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGa          >  2:5433494/1‑101 (MQ=255)
                                                                                            gggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAg         >  1:2686133/1‑101 (MQ=255)
                                                                                             ggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGc        <  1:1263962/101‑1 (MQ=255)
                                                                                              gcgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCt       <  2:3677489/101‑1 (MQ=255)
                                                                                               cgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc      <  2:5668135/101‑1 (MQ=255)
                                                                                                gcgcACGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCg     <  2:1205055/101‑1 (MQ=255)
                                                                                                gcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCg     >  2:3984418/1‑101 (MQ=255)
                                                                                                 cgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg    <  2:738464/101‑1 (MQ=255)
                                                                                                 cgcAAGCGCCGATTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg    >  2:280792/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
CGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCC  >  NC_014034/2155456‑2155655

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG