Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,585 C→G 44.1% L50L (CTC→CTG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5850C→G44.1% 0.0 ‑3.5 ‑0.5389L50L (CTC→CTG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (71/100):  ref base (115/102):  total (186/203)
Fisher's exact test strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.16e-01

Only 100 of 408 total aligned reads displayed.

GGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACG  >  NC_014034/2155485‑2155684
                                                                                                    |                                                                                                   
 ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGctct                                                                                                    <  1:1008377/101‑1 (MQ=255)
 ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCATGAGCCGCGAGCTGctct                                                                                                    >  2:5697242/1‑101 (MQ=255)
  gcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTg                                                                                                   <  1:712083/101‑1 (MQ=255)
      tCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAg                                                                                               <  2:398656/101‑1 (MQ=255)
       caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTCCTCTGGGAGc                                                                                              >  2:3722778/1‑101 (MQ=255)
         ccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTg                                                                                            >  1:3854857/1‑101 (MQ=255)
          cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                                                           >  2:4494418/1‑101 (MQ=255)
          cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                                                           <  1:2110111/101‑1 (MQ=255)
           acGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGcc                                                                                          >  2:4222246/1‑101 (MQ=255)
             gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGGGTGAACTGCTGTGGGAGCTGccgg                                                                                        >  1:1956262/1‑99 (MQ=255)
              aTGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGt                                                                                       >  1:4811513/1‑101 (MQ=255)
              aTGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGt                                                                                       <  2:1282488/101‑1 (MQ=255)
              aTGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccggg                                                                                       >  1:2245426/1‑98 (MQ=255)
                ggcggcggcGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTcc                                                                                     >  2:1988443/1‑100 (MQ=255)
                  cggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTcgcg                                                                                   <  2:2486166/101‑1 (MQ=255)
                   ggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGAGCGGGCGCGCAAGCGACGCTTCCTGAGCCGCGAGCTGCTCTGGGTGCTCCCCGACCCgg                                                                                  >  2:2731270/1‑101 (MQ=255)
                    gcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGt                                                                                 <  1:560066/101‑1 (MQ=255)
                    cgggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGt                                                                                 <  1:3942843/99‑1 (MQ=255)
                     cggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGtg                                                                                <  1:54034/101‑2 (MQ=255)
                        cgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGc                                                                             <  1:5324339/101‑1 (MQ=255)
                         gcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCa                                                                            >  1:1231536/1‑101 (MQ=255)
                         gcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCa                                                                            >  1:444270/1‑101 (MQ=255)
                          cTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCAt                                                                           >  2:3204015/1‑101 (MQ=255)
                           tCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                                          <  2:90471/101‑1 (MQ=255)
                            ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                                                         >  2:204224/2‑101 (MQ=255)
                            ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTACGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                                                         <  1:4921923/100‑1 (MQ=255)
                             gCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGc                                                                        <  2:456088/101‑1 (MQ=255)
                               cGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGa                                                                      >  2:2789043/1‑101 (MQ=255)
                               cGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGa                                                                      >  1:1800720/1‑101 (MQ=255)
                               cGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGa                                                                      >  1:3652357/1‑101 (MQ=255)
                                 ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                                                    >  1:3293969/1‑101 (MQ=255)
                                 ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatc                                                                    >  1:3780658/1‑101 (MQ=255)
                                 ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatc                                                                    <  2:5524664/101‑1 (MQ=255)
                                 ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatc                                                                    <  2:2586617/101‑1 (MQ=255)
                                   cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCTCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                                                  <  2:5145264/101‑1 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGCACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGCCGatcat                                                                  >  2:3128051/4‑101 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                                  <  1:3067992/98‑1 (MQ=255)
                                    ggcTGATGTCGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGCCGGCATGGCGatcatc                                                                 >  2:1892353/1‑101 (MQ=255)
                                    gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                                                 <  2:677423/99‑1 (MQ=255)
                                     gcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCg                                                                >  2:5353186/1‑101 (MQ=255)
                                      cTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCgg                                                               >  1:2592773/1‑101 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                                              >  2:479348/1‑101 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                                              >  1:5722258/1‑101 (MQ=255)
                                        gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGGTTCATGAGCGGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                                             <  1:5697537/101‑1 (MQ=255)
                                        gATGTGGCATGCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                                             <  2:4088103/101‑1 (MQ=255)
                                         aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                                                            >  1:5789244/1‑101 (MQ=255)
                                         aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                                                            <  1:3700726/101‑1 (MQ=255)
                                         aTGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGa                                                            >  2:5290066/1‑101 (MQ=255)
                                          tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                                           >  1:233074/1‑101 (MQ=255)
                                          tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                                           >  1:511053/1‑101 (MQ=255)
                                           gtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAgg                                                          <  2:2163745/101‑1 (MQ=255)
                                            tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                                         >  1:2784128/1‑101 (MQ=255)
                                            tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGc                                                         >  2:3384366/1‑101 (MQ=255)
                                             ggCATTCGACGGAGGTGCGGGCGCGGCGCCGCCGCTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCg                                                        <  2:3565899/101‑1 (MQ=255)
                                             ggCATTCGACCGAGGTGCGGGCGCGAAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCg                                                        <  2:2312985/101‑1 (MQ=255)
                                               cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                                      <  2:4265192/101‑1 (MQ=255)
                                               cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                                      <  1:5765030/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCTGCTGCGCTGTTTTTTCGGGCGTGAACTGCTTTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                                     <  2:3017606/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCggcg                                                     <  2:1183760/101‑3 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                                     <  2:3655800/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                                     <  1:81433/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                                     <  1:5243107/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                                     >  2:4963630/1‑101 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                                     <  1:3588832/101‑1 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                                   <  1:2917688/101‑1 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCGGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                                   <  2:347095/101‑1 (MQ=255)
                                                    gACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcgg                                                 >  2:2673862/1‑101 (MQ=255)
                                                     aCCGAGTTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcggc                                                <  1:939060/101‑1 (MQ=255)
                                                      ccGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCt                                               <  2:5577846/101‑1 (MQ=255)
                                                        gAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                             <  2:3311970/101‑1 (MQ=255)
                                                        gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGNGAGGCGGTGGCGGCTTa                                             >  2:1705373/1‑101 (MQ=255)
                                                         aGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                            >  1:1926910/1‑101 (MQ=255)
                                                          ggTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAtt                                           <  1:127786/101‑1 (MQ=255)
                                                           gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                                          <  2:5779288/101‑1 (MQ=255)
                                                           gTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                                          <  2:5719394/101‑1 (MQ=255)
                                                            tGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTc                                         <  2:1263554/101‑1 (MQ=255)
                                                             gCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                                        <  2:4342912/101‑1 (MQ=255)
                                                             gCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                                        <  2:1346926/101‑1 (MQ=255)
                                                               gggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAg                                      >  1:2686133/1‑101 (MQ=255)
                                                                  cgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc                                   <  1:1692425/101‑1 (MQ=255)
                                                                  cgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc                                   <  1:1184632/101‑1 (MQ=255)
                                                                   gcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCg                                  >  1:1563586/1‑101 (MQ=255)
                                                                    cgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg                                 <  2:1530401/101‑1 (MQ=255)
                                                                      cAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGcc                               >  1:4652438/1‑101 (MQ=255)
                                                                        aGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCgg                             >  2:794491/1‑101 (MQ=255)
                                                                        aGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCgg                             <  1:771785/101‑1 (MQ=255)
                                                                          cgccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAc                           <  1:429453/101‑1 (MQ=255)
                                                                                ttCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac                     >  1:4017013/1‑101 (MQ=255)
                                                                                 tCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacc                    <  1:4191560/101‑1 (MQ=255)
                                                                                   cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                  <  2:1093901/101‑1 (MQ=255)
                                                                                    tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc                 >  1:1444915/1‑101 (MQ=255)
                                                                                     gAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCg                <  1:5073482/101‑1 (MQ=255)
                                                                                     cgggcgtgacctgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCg                <  2:3892567/91‑1 (MQ=255)
                                                                                     cgggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCg                <  2:2908692/91‑1 (MQ=255)
                                                                                      gggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCgg               >  1:5300421/10‑101 (MQ=255)
                                                                                       ggcgtgaactgctGTGGGAGCTGCCGGTGGCCGGGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGc              <  1:1111046/93‑1 (MQ=255)
                                                                                         cgcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgt            <  1:3003753/101‑1 (MQ=255)
                                                                                              gctgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGtcgtcg       >  1:4559470/1‑101 (MQ=255)
                                                                                               ctgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGc      >  2:3007285/1‑101 (MQ=255)
                                                                                                tgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGcc     <  1:3445165/101‑2 (MQ=255)
                                                                                                    |                                                                                                   
GGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACG  >  NC_014034/2155485‑2155684

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG