Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,995	G→C	100%	intergenic (+268/‑237)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,995	0	G→C	100.0%	10.2	6	intergenic (+268/‑237)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (0/0):  total (0/6)

AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGC  >  NC_014034/2185919‑2186078                                                                                |                                                                                    accgcgcaaCCGCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCCGGGGGCCGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAccc                                                              <  2:696448/94‑1 (MQ=2)         accgcccccTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCa                                                      <  1:1668203/96‑1 (MQ=0)                        cTTGGCTCAAATATCCCGGGGGCCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                                       <  2:2492220/101‑1 (MQ=9)                                                     gcggagcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc          <  2:1238457/93‑1 (MQ=21)                                                           cccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACg    <  1:2578370/99‑1 (MQ=25)                                                             cccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGc  <  1:4308924/101‑1 (MQ=25)                                                                                |                                                                                    AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGC  >  NC_014034/2185919‑2186078 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG