Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,012	G→A	100%	intergenic (+285/‑220)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,012	0	G→A	100.0%	223.2	58	intergenic (+285/‑220)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (37/21):  ref base (0/0):  total (37/21)

AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185919‑2186109                                                                                                 |                                                                                                  accgcgcaaCCGCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCCGGGGGCCGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAccc                                                                                             <  2:696448/94‑1 (MQ=2)         accgcccccTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCa                                                                                     <  1:1668203/96‑1 (MQ=0)                        cTTGGCTCAAATATCCCGGGGGCCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                                                                      <  2:2492220/101‑1 (MQ=9)                                                     gcggagcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc                                         <  2:1238457/93‑1 (MQ=21)                                                           cccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACg                                   <  1:2578370/99‑1 (MQ=25)                                                             cccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGc                                 <  1:4308924/101‑1 (MQ=25)                                                                          gcctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGAGCAAAAGCCCGGCCCGGCAGGACGGCTATGCCTTGAc                      >  1:4035153/11‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTTCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     >  1:3556549/10‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     >  1:5263791/10‑101 (MQ=255)                                                                           cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     <  2:702756/92‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    <  2:1977690/93‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    <  1:2196477/93‑1 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  2:1187555/9‑101 (MQ=255)                                                                            ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  2:3019161/9‑101 (MQ=255)                                                                             tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   <  1:2090434/94‑1 (MQ=255)                                                                             tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   <  2:3001313/94‑1 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCTTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:887951/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcccag                  >  1:1977801/7‑99 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:5486693/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  2:5270947/7‑101 (MQ=255)                                                                              cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:16237/7‑101 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  2:554090/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  1:4094340/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  1:4646275/96‑1 (MQ=255)                                                                               gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  2:4270070/96‑1 (MQ=255)                                                                               cggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  2:5043085/6‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  1:1652462/5‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  1:1784724/5‑101 (MQ=255)                                                                                ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  1:322259/5‑101 (MQ=255)                                                                                  caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa              >  2:3024306/3‑101 (MQ=255)                                                                                  caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa              >  1:4160221/3‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  2:2621596/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  2:4844865/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  2:5229369/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  1:3509379/2‑101 (MQ=255)                                                                                   aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  1:1746561/2‑101 (MQ=255)                                                                                    aaGCCCGAGGGGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            <  1:2097996/101‑1 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  2:1068229/1‑101 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  2:628929/1‑101 (MQ=255)                                                                                    aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            <  1:1399098/101‑1 (MQ=255)                                                                                     agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg           <  1:4515177/98‑1 (MQ=255)                                                                                      gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg          <  2:2224994/99‑1 (MQ=255)                                                                                      gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg          <  2:4191139/99‑1 (MQ=255)                                                                                       cccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCggg         >  2:5114733/2‑101 (MQ=255)                                                                                        ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        <  1:2773520/101‑1 (MQ=255)                                                                                        ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  1:3450407/1‑101 (MQ=255)                                                                                        ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCAGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  2:2640695/1‑101 (MQ=255)                                                                                         cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  1:342461/1‑101 (MQ=255)                                                                                         cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  1:5337882/1‑101 (MQ=255)                                                                                          gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  1:3646402/1‑101 (MQ=255)                                                                                          gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  1:1461487/1‑101 (MQ=255)                                                                                           aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  2:2934074/1‑101 (MQ=255)                                                                                           aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  2:5140286/1‑101 (MQ=255)                                                                                           aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  1:3710046/1‑101 (MQ=255)                                                                                            ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    >  2:4757483/1‑101 (MQ=255)                                                                                             gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   <  2:4359979/101‑1 (MQ=255)                                                                                             gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   >  1:3654106/1‑101 (MQ=255)                                                                                              cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgggga  >  2:383791/1‑99 (MQ=255)                                                                                              cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  <  1:992538/101‑1 (MQ=255)                                                                                              cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  >  1:3582462/1‑101 (MQ=255)                                                                                                 |                                                                                                  AACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185919‑2186109 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG