Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,210,243 T→A 100% V102D (GTT→GAT)  cobQ1 → cobyric acid synthase CobQ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,210,2430T→A100.0% 1026.0 273V102D (GTT→GAT) cobQ1cobyric acid synthase CobQ
Reads supporting (aligned to +/- strand):  new base (137/136):  ref base (0/0):  total (137/136)

Only 100 of 280 total aligned reads displayed.

CGTGACCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGTTTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAGGGCGCGGGC  >  NC_014034/2210145‑2210340
                                                                                                  |                                                                                                 
  tGACCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTAt                                                                                               >  2:2558710/1‑101 (MQ=255)
   gACCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATg                                                                                              >  2:3122437/1‑101 (MQ=255)
    aCCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGc                                                                                             <  1:3704891/101‑1 (MQ=255)
    aCCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGc                                                                                             <  2:593126/101‑1 (MQ=255)
     ccGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCg                                                                                            <  1:2301571/101‑1 (MQ=255)
      cGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGCCGTTGGTGCGCAGGTCGAGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGa                                                                                           >  1:740674/1‑101 (MQ=255)
      cGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGa                                                                                           >  2:2062125/1‑101 (MQ=255)
      cGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGa                                                                                           >  2:4551201/1‑101 (MQ=255)
       gACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGAc                                                                                          >  1:5174243/1‑101 (MQ=255)
          aTGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCt                                                                                       >  2:1070681/1‑101 (MQ=255)
          aTGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCt                                                                                       <  1:1936115/101‑1 (MQ=255)
          aTGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCt                                                                                       <  2:3327123/101‑1 (MQ=255)
          aTGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCt                                                                                       <  1:4105226/101‑1 (MQ=255)
           tGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTg                                                                                      <  1:2829004/101‑1 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     >  1:5305749/1‑101 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     <  1:4461682/101‑1 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     >  2:5096149/1‑101 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     >  1:5327436/1‑101 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     >  1:5785389/1‑101 (MQ=255)
            gAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGa                                                                                     >  1:1430621/1‑101 (MQ=255)
             aaCCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGaa                                                                                    >  1:3198682/1‑101 (MQ=255)
               cccGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAAc                                                                                  >  2:2419159/1‑101 (MQ=255)
               cccCATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAAc                                                                                  <  2:3813432/101‑1 (MQ=255)
                ccGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAAcc                                                                                 <  1:4315272/101‑1 (MQ=255)
                ccGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAAcc                                                                                 <  1:3507753/101‑1 (MQ=255)
                 cGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCg                                                                                <  1:181563/101‑1 (MQ=255)
                 cGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGAGATTATGCGACGCTGAAACCg                                                                                <  1:4254895/101‑1 (MQ=255)
                   ttgatcagaaaGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCa                                                                              >  2:3550217/10‑101 (MQ=255)
                   aTCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCa                                                                              <  1:4773473/101‑1 (MQ=255)
                    tCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAg                                                                             >  1:1163484/1‑101 (MQ=255)
                    tCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAg                                                                             >  2:1197564/1‑101 (MQ=255)
                       tctTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTg                                                                          <  1:65766/101‑1 (MQ=255)
                        ctTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGa                                                                         >  1:5398060/1‑101 (MQ=255)
                         ttGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGAt                                                                        <  1:3989634/101‑1 (MQ=255)
                         ttGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGAt                                                                        <  2:1906914/101‑1 (MQ=255)
                          tGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATg                                                                       >  1:2697999/1‑101 (MQ=255)
                            aaGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATggg                                                                     >  1:2068489/1‑101 (MQ=255)
                              gCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATggggg                                                                   <  2:1159487/101‑1 (MQ=255)
                               cccGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATgggggg                                                                  >  2:2728132/1‑100 (MQ=255)
                                 cGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCgg                                                                >  2:4104668/1‑101 (MQ=255)
                                  gAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGt                                                               <  2:5422279/101‑1 (MQ=255)
                                  gAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGt                                                               <  2:4680178/101‑1 (MQ=255)
                                    aaGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTc                                                             <  1:3087923/101‑1 (MQ=255)
                                    aaGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTc                                                             >  2:1687605/1‑101 (MQ=255)
                                     aGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCt                                                            >  1:5461556/1‑101 (MQ=255)
                                     aGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCt                                                            >  2:5305797/1‑101 (MQ=255)
                                     aGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCt                                                            <  1:3437129/101‑1 (MQ=255)
                                       tGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTgg                                                          <  1:5140257/101‑1 (MQ=255)
                                         aCGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGaa                                                        >  2:1408958/1‑101 (MQ=255)
                                         aCGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGaa                                                        >  2:3241232/1‑101 (MQ=255)
                                           gTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAg                                                      >  2:266507/1‑101 (MQ=255)
                                           gTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAg                                                      >  2:5739432/1‑101 (MQ=255)
                                           gTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAg                                                      >  2:5651923/1‑101 (MQ=255)
                                            ttGGTGCGCAGGTCGTGGTGCAGGGCCAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGt                                                     <  1:4861886/101‑1 (MQ=255)
                                            ttGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGt                                                     <  1:2877760/101‑1 (MQ=255)
                                                tGCGCGGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCa                                                 <  2:4877141/101‑1 (MQ=255)
                                                tGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCa                                                 >  1:2981456/1‑101 (MQ=255)
                                                  cgcAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAAc                                               >  2:2517117/1‑101 (MQ=255)
                                                    cAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCg                                             <  2:289428/101‑1 (MQ=255)
                                                    cAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCg                                             >  2:5030140/1‑101 (MQ=255)
                                                        tCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTc                                         <  2:2075332/101‑1 (MQ=255)
                                                         cGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCa                                        <  1:463056/101‑1 (MQ=255)
                                                          gtggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCaa                                       >  2:1789956/1‑101 (MQ=255)
                                                          gtggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCaa                                       >  2:2227244/1‑101 (MQ=255)
                                                          gtggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCaa                                       <  2:2232328/101‑1 (MQ=255)
                                                           tggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAg                                      <  1:3375231/101‑1 (MQ=255)
                                                            ggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAgg                                     <  1:2153994/101‑1 (MQ=255)
                                                            ggtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAgg                                     >  1:1085104/1‑101 (MQ=255)
                                                             gtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGc                                    <  1:2229229/101‑1 (MQ=255)
                                                             gtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGc                                    <  1:3031115/101‑1 (MQ=255)
                                                             gtgCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGc                                    >  2:1491576/1‑101 (MQ=255)
                                                                cAGGGCAAGCGGCTCACCACCACGCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGAc                                 <  1:3796211/101‑1 (MQ=255)
                                                                  gggCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGAccc                               <  1:2005392/101‑1 (MQ=255)
                                                                   ggCAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCa                              >  1:1223413/1‑101 (MQ=255)
                                                                    gCAAGCGGCTCACCACCCCCCGGGCGCGCGATTATGCGAGGCTGAAACCGCTGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCAt                             <  2:671684/101‑1 (MQ=255)
                                                                     cAAGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATg                            >  2:2109283/1‑101 (MQ=255)
                                                                       aGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGAt                          >  2:3940752/1‑101 (MQ=255)
                                                                       aGCGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGAt                          >  2:3025260/1‑101 (MQ=255)
                                                                         ccgCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCt                        <  2:3904585/99‑1 (MQ=255)
                                                                         cGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCt                        >  2:4330557/1‑101 (MQ=255)
                                                                         cGGCTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCt                        <  2:885388/101‑1 (MQ=255)
                                                                            cTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGt                     >  2:1561342/1‑101 (MQ=255)
                                                                            cTCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCCGCTCACAGAGACCCaccacccggc                     >  2:4971523/1‑92 (MQ=255)
                                                                             tCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTg                    >  1:844939/1‑101 (MQ=255)
                                                                             tCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTg                    <  1:3971438/101‑1 (MQ=255)
                                                                             tCACCACCACCCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTg                    >  1:2137345/1‑101 (MQ=255)
                                                                               accaccaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGGCCCATGATCTGGTGAt                  <  1:1491669/101‑1 (MQ=255)
                                                                                ccaccaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtc                 >  1:3912593/1‑101 (MQ=255)
                                                                                ccaccaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtc                 <  1:2162874/101‑1 (MQ=255)
                                                                                 caccaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtcg                <  2:4640976/101‑1 (MQ=255)
                                                                                  accaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtcgt               <  2:3770063/101‑1 (MQ=255)
                                                                                  accaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtcgt               <  1:866587/101‑1 (MQ=255)
                                                                                  accaccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtcgt               >  1:2548513/1‑101 (MQ=255)
                                                                                    caccCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGAtcgtcg             >  2:4756456/1‑101 (MQ=255)
                                                                                     accCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGa            <  1:5213594/101‑1 (MQ=255)
                                                                                     accCGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGa            >  1:1211381/1‑101 (MQ=255)
                                                                                      cccGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAg           <  2:246383/101‑1 (MQ=255)
                                                                                       ccGGGCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAgg          >  1:1014319/1‑101 (MQ=255)
                                                                                          ggCGCGCGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAGGgcg       >  2:1514638/1‑101 (MQ=255)
                                                                                           gcgcgcGATTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAGGgcgc      <  1:4504587/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGTGACCGACATGAACCCGATCCTCTTGAAGCCCGAAAGTGACGTTGGTGCGCAGGTCGTGGTGCAGGGCAAGCGGCTCACCACCACCCGGGCGCGCGTTTATGCGACGCTGAAACCGCAGCTGATGGGGGCGGTTCTGGAAAGTTTCAACCGGCTCAAGGCGACCCATGATCTGGTGATCGTCGAGGGCGCGGGC  >  NC_014034/2210145‑2210340

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG