Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 3.9% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 3.9% 21.9 ‑0.9 ‑0.2306intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (131/163):  total (138/168)
Fisher's exact test strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.80e-01

Only 100 of 318 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACG  >  NC_014034/2249217‑2249415
                                                                                                      |                                                                                                             
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                               >  1:5704543/1‑101 (MQ=255)
   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                            >  1:1001972/1‑101 (MQ=255)
     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                          <  2:157140/101‑1 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                         <  2:347935/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                      <  2:4962380/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                      <  1:1856827/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                     <  2:2506593/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                     >  2:4832111/1‑101 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                    <  1:4074542/101‑1 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                    <  2:4695530/101‑1 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                    <  2:5031176/101‑1 (MQ=255)
            tGAGATGCACAGATCCCGCAGCTTGGGCACCGGGGGCAAGAA.GGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               <  1:65840/101‑1 (MQ=38)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               >  2:1214339/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               >  2:246349/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               >  1:4559691/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               >  1:787359/1‑101 (MQ=255)
              agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                              <  2:2370016/101‑1 (MQ=255)
               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                             >  1:420930/1‑101 (MQ=255)
               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                             <  1:1922486/101‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                         <  2:1659645/101‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCAGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGGC...gggg..gg                                                                                         >  1:440113/1‑101 (MQ=39)
                   cacaGATACCGCATCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       <  1:2854235/101‑1 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       >  2:1964935/1‑101 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       <  2:10969/101‑1 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       <  1:1120856/101‑1 (MQ=255)
                   cacaGAAACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                       <  1:959722/101‑1 (MQ=255)
                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                      >  2:4342402/1‑101 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                     >  1:5615551/1‑101 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                    >  1:3071001/1‑101 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                  <  2:1781233/101‑1 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                  <  2:4049277/101‑1 (MQ=255)
                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                <  2:3311615/101‑1 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                               <  1:382068/101‑1 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                               <  1:1316035/101‑1 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                               <  1:1076568/101‑1 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                              <  1:332820/101‑1 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                              <  2:2963652/101‑1 (MQ=255)
                            cGCAGCTGGGGCCCCGTGGGCAAGAAGGGGGATCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                              <  2:804596/101‑1 (MQ=37)
                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                             >  1:4278022/1‑101 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                            >  2:2206460/1‑101 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                            >  2:509718/1‑101 (MQ=255)
                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGACATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                          >  1:5105964/1‑101 (MQ=255)
                                gCTTGGGCACCCGGGGCAAGAAGGGGCCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                          <  2:3764183/101‑1 (MQ=255)
                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                        >  1:4238100/1‑101 (MQ=255)
                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                       >  2:3555088/1‑101 (MQ=255)
                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                       >  1:2212618/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                      <  2:3637712/101‑1 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                      >  2:4113417/1‑101 (MQ=255)
                                     ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                     <  1:5451581/101‑1 (MQ=255)
                                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                    <  1:265060/101‑1 (MQ=255)
                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGGAAATATcc                                                                  >  1:153183/1‑101 (MQ=255)
                                         cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                 <  1:49086/101‑1 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                <  1:46576/101‑1 (MQ=255)
                                             gggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                             <  2:5329162/101‑1 (MQ=255)
                                              ggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                            <  2:4536711/101‑1 (MQ=255)
                                                gaagaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                          <  1:1483221/100‑1 (MQ=255)
                                                cAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                          <  1:2533352/101‑1 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCAggggggag                                                         >  1:2369400/1‑99 (MQ=38)
                                                 aagaagGGGGCTCTGCCCCC.GTCCCGGGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                         <  1:2059922/101‑1 (MQ=255)
                                                   gaagGGGGCTCGGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                       <  1:2945530/101‑1 (MQ=255)
                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                     >  2:1497481/1‑101 (MQ=255)
                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                     <  2:5175881/101‑1 (MQ=255)
                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCCGCACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                     <  1:2256815/101‑1 (MQ=37)
                                                     agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                    <  2:2840027/101‑1 (MQ=255)
                                                       ggggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                  <  2:4943000/101‑1 (MQ=255)
                                                         ggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTAATCTTGCCGAAAATATCCCGGGGGGGGAA.TGgcgc                                                >  1:1037231/1‑101 (MQ=38)
                                                          gctctcccccc.GTCCCGTGCCGGGACC.CCCCCGGGATTTTTGAGGAAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                               <  1:169553/94‑1 (MQ=37)
                                                             cggccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCGAGATGAA...GGTG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                          <  2:4812906/97‑1 (MQ=255)
                                                              tgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                         <  1:3618486/98‑1 (MQ=255)
                                                              tgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                         <  2:3915410/98‑1 (MQ=255)
                                                              tcccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..CCCa                                         <  2:4908152/98‑1 (MQ=255)
                                                               gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                        >  2:3855116/3‑101 (MQ=255)
                                                                 cccc.GTCCCGGGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                      <  2:2080871/101‑1 (MQ=255)
                                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                 <  2:4370485/101‑1 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCCGAGGGGGGCa                               >  2:2572536/1‑101 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                               >  2:2728021/1‑101 (MQ=255)
                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                              <  2:4629769/101‑1 (MQ=255)
                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                             >  1:3575561/1‑101 (MQ=255)
                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                             >  2:60989/1‑101 (MQ=255)
                                                                            tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                            >  2:5691173/1‑101 (MQ=255)
                                                                            tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                            >  2:5475188/1‑101 (MQ=255)
                                                                            caagcggACTCCCCCCGGGATATTTGCAGCAAGCTGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                <  1:4983535/96‑1 (MQ=18)
                                                                              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                         <  2:171451/101‑1 (MQ=255)
                                                                              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                         >  2:2292392/1‑101 (MQ=255)
                                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                        >  1:5282299/1‑101 (MQ=255)
                                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                        >  1:1279654/1‑101 (MQ=255)
                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                   <  1:3172465/101‑4 (MQ=255)
                                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  >  2:4185741/1‑97 (MQ=255)
                                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                  >  1:3511685/1‑97 (MQ=255)
                                                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTcgc                >  2:3213230/1‑101 (MQ=255)
                                                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTcgc                >  2:5608327/1‑101 (MQ=255)
                                                                                         cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTcgcg               >  2:1122441/1‑101 (MQ=255)
                                                                                           cgcgATAATAGAGGCAAGCTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCATTCgcggg             <  2:2155548/98‑1 (MQ=25)
                                                                                           cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcggg             <  1:3766694/101‑1 (MQ=255)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc            <  1:3268213/101‑1 (MQ=255)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc            <  2:4246316/101‑1 (MQ=255)
                                                                                              gATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgccccgc             >  2:4463974/1‑94 (MQ=2)
                                                                                              gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcgg          >  1:4712468/1‑101 (MQ=255)
                                                                                               atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGggcggc         >  2:5111094/1‑101 (MQ=255)
                                                                                                   ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg     <  2:1011146/101‑1 (MQ=255)
                                                                                                      |                                                                                                             
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACG  >  NC_014034/2249217‑2249415

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG