Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,330 G→A 3.0% intergenic (‑126/‑179) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3300G→A 3.0% 9.1 ‑0.3 ‑0.0256intergenic (‑126/‑179)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (114/133):  total (117/139)
Fisher's exact test strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 290 total aligned reads displayed.

AGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGG  >  NC_014034/2249231‑2249429
                                                                                                         |                                                                                                          
agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                                            <  2:2370016/101‑1 (MQ=255)
agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                                            >  2:428594/1‑101 (MQ=255)
 gaTGCACAGATCCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                           <  1:5258103/101‑1 (MQ=255)
 gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                           <  2:2146186/101‑1 (MQ=255)
 gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                           >  1:3809745/1‑101 (MQ=255)
   tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                       >  1:3335318/1‑101 (MQ=255)
   tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                       <  2:1659645/101‑1 (MQ=255)
    gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGc                                                                                                      >  2:5451291/1‑101 (MQ=255)
     cacaGATACCGCATCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                                     <  1:2854235/101‑1 (MQ=255)
     cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                                     <  2:10969/101‑1 (MQ=255)
       caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                   <  2:3250054/101‑1 (MQ=255)
       caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                   <  1:2057049/101‑1 (MQ=255)
       caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                   <  1:4700531/101‑1 (MQ=255)
        aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  >  1:3071001/1‑101 (MQ=255)
          aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                                >  2:49075/1‑101 (MQ=255)
          aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                                <  2:1781233/101‑1 (MQ=255)
          aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                                <  2:3612529/101‑1 (MQ=255)
            aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              <  1:5029491/101‑1 (MQ=255)
            aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              >  1:3582926/1‑101 (MQ=255)
            aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                              >  2:2059805/1‑101 (MQ=255)
              cGCAGCTGGGGCCCCGTGGGCAAGAAGGGGGATCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                                            <  2:804596/101‑1 (MQ=37)
               gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                           <  1:5171188/101‑1 (MQ=255)
                cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                                          >  2:4923532/1‑101 (MQ=255)
                 aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                         <  1:4109309/101‑1 (MQ=255)
                   cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                       >  1:746155/1‑101 (MQ=255)
                     tGGGCACGCGGGGCAAGAAATGGGCTCT.GCCCCCGTCCCGTGGCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGAG..GGCTTTCATCTTGCCGaaaa                                                                                     <  1:3279869/101‑1 (MQ=25)
                     tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                     <  2:4824643/101‑1 (MQ=255)
                       ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                   <  1:5451581/101‑1 (MQ=255)
                          aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGGAAATATcc                                                                                >  1:153183/1‑101 (MQ=255)
                          aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTCCCGAAAATATcc                                                                                <  1:3126609/101‑1 (MQ=255)
                             cGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                             <  1:2253609/101‑1 (MQ=255)
                               gggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                                           >  2:5588243/1‑101 (MQ=255)
                                ggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                          >  2:2530781/1‑101 (MQ=255)
                                ggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                          <  2:4536711/101‑1 (MQ=255)
                                  gaagcagGGGGCTCTCC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                        <  1:912409/100‑1 (MQ=37)
                                  gaagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                        <  1:1483221/100‑1 (MQ=255)
                                  cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                        <  1:5671797/101‑1 (MQ=255)
                                   aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                       >  1:1957463/1‑99 (MQ=255)
                                   aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                       >  1:2575173/1‑99 (MQ=255)
                                   aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCAGAAAATATCCCGGGGGGTg                                                                       <  1:4499628/101‑1 (MQ=255)
                                   aagaagGGGGCTCT.GCCCCCGTCCAGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                       >  2:2474096/1‑101 (MQ=255)
                                    agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                      <  2:3869322/101‑1 (MQ=255)
                                    agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                      <  1:2353482/101‑1 (MQ=255)
                                     gaagGGGGCTCG.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                     <  1:2945530/101‑1 (MQ=255)
                                      aatggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                   >  2:4143474/4‑101 (MQ=255)
                                      aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                   >  2:1497481/1‑101 (MQ=255)
                                       agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                  <  2:2840027/101‑1 (MQ=255)
                                       agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                  >  2:2174038/1‑101 (MQ=255)
                                         ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                <  2:4654860/101‑1 (MQ=255)
                                           ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                              <  1:3463851/101‑1 (MQ=255)
                                           ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                              >  2:5143000/1‑101 (MQ=255)
                                            gCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                             >  1:4309378/1‑101 (MQ=255)
                                             gctccgcccccGTCCCGTGCCGGGACT.CCCCTGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                             <  2:2693487/94‑1 (MQ=255)
                                             ctct.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                          <  2:3985903/101‑1 (MQ=255)
                                                ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                        >  1:3558700/5‑101 (MQ=255)
                                                 tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                       <  2:5077108/98‑1 (MQ=255)
                                                       cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                 >  2:475349/1‑101 (MQ=255)
                                                        gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                <  1:251357/101‑1 (MQ=255)
                                                         tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                               <  2:4370485/101‑1 (MQ=255)
                                                           ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                             <  1:4941439/101‑1 (MQ=255)
                                                            cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                            >  1:374616/1‑101 (MQ=255)
                                                            cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                            <  2:5103290/101‑1 (MQ=255)
                                                            cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                >  2:3850774/8‑101 (MQ=18)
                                                             gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                          <  1:2671209/101‑1 (MQ=255)
                                                             gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                          >  2:4357325/1‑101 (MQ=255)
                                                             gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                          >  2:60989/1‑101 (MQ=255)
                                                             gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                               <  2:5238796/95‑1 (MQ=18)
                                                              tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                         >  2:5691173/1‑101 (MQ=255)
                                                                ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                      <  2:171451/101‑1 (MQ=255)
                                                                ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                      >  2:2292392/1‑101 (MQ=255)
                                                                  gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                    >  1:1861457/1‑101 (MQ=255)
                                                                   ggccT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                   <  1:1024759/98‑1 (MQ=255)
                                                                   ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGCGAA..GCCAGAGGGGGGCTg.cg.c                                       >  2:4956631/1‑101 (MQ=17)
                                                                   ggACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                   <  2:5615259/101‑1 (MQ=255)
                                                                   ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                   >  2:3739006/1‑101 (MQ=255)
                                                                   ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                   >  1:4269649/1‑101 (MQ=255)
                                                                   ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccc                                   >  2:4051320/1‑101 (MQ=255)
                                                                      cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccctt                                <  1:4220541/101‑4 (MQ=255)
                                                                       t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                               <  2:3468414/101‑5 (MQ=255)
                                                                           cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgc                                >  1:1041015/1‑99 (MQ=2)
                                                                           cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgcg                             >  2:1122441/1‑101 (MQ=255)
                                                                              gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                          >  1:2198455/1‑101 (MQ=255)
                                                                               ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggcg                         >  1:1103886/1‑101 (MQ=255)
                                                                                  ttttggAGGAAAGAAAAG...GGGG..GGTTTTAATCTTGCCAAAAATATCCGGGGGGGTGAA.TGGCGCA..GCAAGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGcc                      <  2:1175788/96‑1 (MQ=255)
                                                                                  tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                      <  1:5614770/101‑1 (MQ=255)
                                                                                  tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                      <  1:1374223/101‑1 (MQ=37)
                                                                                    tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgc                    >  2:2914001/1‑101 (MQ=255)
                                                                                     ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgcg                   <  2:1011146/101‑1 (MQ=255)
                                                                                      tGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGa                  >  1:1217705/1‑101 (MQ=255)
                                                                                          gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcg              <  2:2212496/101‑1 (MQ=255)
                                                                                           cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc             >  1:4014290/1‑101 (MQ=255)
                                                                                              gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc          >  1:5389961/1‑101 (MQ=255)
                                                                                              gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc          <  2:3335113/101‑1 (MQ=255)
                                                                                                 gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa       >  2:3033965/1‑101 (MQ=255)
                                                                                                 gAA...GGGG..GGCTTTCATCTGGCCGAAAATGTCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa       <  1:475399/101‑1 (MQ=37)
                                                                                                  aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa      >  1:1283618/1‑101 (MQ=255)
                                                                                                  aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa      >  1:3178166/1‑101 (MQ=255)
                                                                                                   a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc     <  1:1692353/101‑1 (MQ=255)
                                                                                                   a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc     <  2:1951910/101‑1 (MQ=255)
                                                                                                        ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg   <  2:4467326/101‑1 (MQ=255)
                                                                                                         |                                                                                                          
AGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGG  >  NC_014034/2249231‑2249429

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG