BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,003	A→G	7.5%	intergenic (+51/‑191)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,003	0	A→G	7.5%	25.1 ‑3.2 ‑0.0	186	intergenic (+51/‑191)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (9/5): ref base (58/113): total (68/118)
Fisher's exact test strand distribution p-value = 3.92e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 208 total aligned reads displayed.


GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGG.G.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCG  >  NC_014034/2493904‑2494100


gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgca                                                                                                        <  2:5335120/101‑1 (MQ=25)


  gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.GGCTGCCCCCCGTCCGCAAGCGGGCCCCCCCCCGGGTATTTgcagc                                                                                                      >  1:4743777/1‑101 (MQ=25)


  gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAACCGGACTCCCCCCGGGGAATTTgccgc                                                                                                      >  1:1147824/1‑101 (MQ=21)


   tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                                                                     <  1:1295757/101‑1 (MQ=25)


   tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                                                                     <  2:4712387/101‑1 (MQ=25)


    cTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                                                                                    <  2:1850605/101‑1 (MQ=25)


     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.GGCTGCCCCCCGTCCGCAAGGGGAATCCCCCCAGGAAATTTGGCGCaaa                                                                                                   >  1:2268630/1‑100 (MQ=18)


     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                                                                   <  2:716772/101‑1 (MQ=21)


      ggCCCCGCGCCGCGGCGGGCGCGCGGCACGACCGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                                                                  <  1:792750/101‑1 (MQ=18)


      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.GGCGGGCCCCCCGCCGCCCGCGGACCCCCCCCCGGGGATTTTCCGGAAGa                                                                                                  >  1:3747665/1‑101 (MQ=255)


      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                                                                  <  2:4013621/101‑1 (MQ=21)


      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                                                                  <  1:2897694/101‑1 (MQ=21)


       gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                                                                 <  2:4055277/101‑1 (MQ=21)


       gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                                                                 <  1:5516441/101‑1 (MQ=21)


        cccCGCGCCGCGGCGGGCGGGCGGCAGGACAGGCCGAGTAA..GGGGGGCG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                                                                                <  1:2682086/101‑1 (MQ=18)


         ccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGa                                                                                               >  1:1935271/1‑101 (MQ=21)


            gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                            <  2:2526510/101‑1 (MQ=21)


             cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                           <  2:4360332/101‑1 (MQ=21)


             cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                           <  1:2709425/101‑1 (MQ=21)


              gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                          <  1:2046516/101‑1 (MQ=21)


                 gcggcgGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                       <  2:5533574/101‑1 (MQ=21)


                 gcggcgGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                       <  2:2164660/101‑1 (MQ=21)


                  cggcggGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                      <  2:3747437/101‑1 (MQ=21)


                   ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                     <  1:1229830/101‑1 (MQ=21)


                     cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                   <  1:5606574/101‑1 (MQ=21)


                      gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                  <  2:4272241/101‑1 (MQ=21)


                      gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                  <  1:3340066/101‑1 (MQ=21)


                      gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                  >  2:2446846/1‑101 (MQ=21)


                      gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCCGGACATTTGCAGCCAGATGAAAACGGAGGccgt                                                                                  >  1:5716720/1‑99 (MQ=18)


                       gggggggggCCCGCCAGGCCGAGTAAG.GGGGGG.G.CGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                 <  1:3210721/94‑1 (MQ=17)


                       ggcgTGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                 >  1:5239086/1‑101 (MQ=18)


                       ggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                 <  1:1091910/101‑1 (MQ=21)


                        gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                 <  2:4441272/101‑1 (MQ=18)


                         cgcgcgGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                               >  1:1526278/1‑101 (MQ=21)


                          gcgcgGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                              <  1:5128591/101‑1 (MQ=21)


                           cgcgGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                             <  2:2335079/101‑1 (MQ=21)


                            gcgGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                            <  2:3431272/101‑1 (MQ=21)


                             cgGCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                           <  2:4126816/101‑1 (MQ=21)


                              ggCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                          <  1:4043740/101‑1 (MQ=21)


                              ggCACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                          <  1:166654/101‑1 (MQ=21)


                                cACGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                        >  2:5366437/1‑101 (MQ=21)


                                 aCGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                       <  2:4743496/101‑1 (MQ=21)


                                 aCGACAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                       >  1:2371653/1‑101 (MQ=21)


                                     cAGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                   <  2:3984427/101‑1 (MQ=21)


                                      aGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                  <  2:3911735/101‑1 (MQ=21)


                                      aGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                  <  2:4539795/101‑1 (MQ=21)


                                      aGGCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                  <  2:4629544/101‑1 (MQ=21)


                                        gCCGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                <  1:3597734/101‑1 (MQ=21)


                                        gCCGAGTAA..GGGGGG.GCCGCTGCCCCCCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                <  2:5644917/101‑1 (MQ=21)


                                         ccGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                               <  2:5480580/101‑1 (MQ=21)


                                         ccGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                               <  2:329755/101‑1 (MQ=21)


                                         ccGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                               >  2:5270971/1‑101 (MQ=18)


                                          cGAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                              <  1:1768512/101‑1 (MQ=21)


                                           gAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                             >  1:5668494/1‑101 (MQ=18)


                                           gAGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                             >  1:1109696/1‑101 (MQ=18)


                                            aGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCCAGCGGACTCCCACCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                            <  2:2268504/101‑1 (MQ=18)


                                            aGTAAG.GGGGGG.G.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                            >  1:1711471/1‑101 (MQ=18)


                                              gtaaggggggg.g.CGCGGCCCACCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                           <  2:1457348/96‑1 (MQ=18)


                                               taaggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                          >  1:4504453/5‑101 (MQ=255)


                                                aaggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                         >  2:190980/4‑101 (MQ=255)


                                                aaggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                         <  2:900536/98‑1 (MQ=255)


                                                 aggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                        <  1:2298560/99‑1 (MQ=255)


                                                 aggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                        >  2:3237842/3‑101 (MQ=255)


                                                 aggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCGGGGTTTTTGCCGCCAGCTGACACCGGATGCCCTTCATCTTCCCCTAAATAATCCggggg                                                        >  2:2288534/3‑101 (MQ=12)


                                                  ggggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                       <  1:4207758/100‑1 (MQ=255)


                                                   gggggg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                      <  2:4936145/101‑1 (MQ=255)


                                                     gggc.g.CGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                    <  2:5703803/101‑1 (MQ=18)


                                                       gg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                 <  1:1563632/101‑1 (MQ=255)


                                                       gg.g.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                 <  1:1688307/101‑1 (MQ=255)


                                                             gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                           >  2:3684816/1‑101 (MQ=255)


                                                               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                        >  1:5283323/1‑100 (MQ=255)


                                                                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aac                                       >  1:5799507/1‑99 (MQ=18)


                                                                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                       <  1:4336343/101‑3 (MQ=255)


                                                                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                     <  2:3399449/101‑5 (MQ=255)


                                                                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                    <  1:4722280/101‑6 (MQ=255)


                                                                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                 <  1:2977745/99‑9 (MQ=255)


                                                                        tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                               <  1:2566573/101‑11 (MQ=255)


                                                                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                           <  2:1919512/101‑1 (MQ=18)


                                                                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                         >  1:101779/1‑99 (MQ=18)


                                                                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                        >  2:2783474/1‑98 (MQ=18)


                                                                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                        >  2:128492/1‑98 (MQ=18)


                                                                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                       >  1:2080257/1‑97 (MQ=18)


                                                                                  gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                    >  2:596877/1‑101 (MQ=18)


                                                                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc                   >  1:2841308/1‑101 (MQ=18)


                                                                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                >  2:2990638/1‑100 (MQ=17)


                                                                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                >  2:1216098/1‑100 (MQ=18)


                                                                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGccc            <  1:2517749/101‑1 (MQ=17)


                                                                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGccc            >  1:3401375/1‑101 (MQ=17)


                                                                                            gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc           >  2:5233772/1‑101 (MQ=0)


                                                                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGAGC.AGCGCCCCCCTGCCCcg          >  1:3456871/1‑101 (MQ=17)


                                                                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg         >  1:5353248/1‑101 (MQ=18)


                                                                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg         >  1:5398684/1‑101 (MQ=17)


                                                                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc        >  1:97697/1‑99 (MQ=2)


                                                                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc        >  2:1511896/1‑99 (MQ=2)


                                                                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc        <  2:3021862/101‑3 (MQ=2)


                                                                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc        >  2:1738181/1‑101 (MQ=17)


                                                                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc        >  2:2837301/1‑101 (MQ=17)


                                                                                                tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccg       >  2:496992/1‑101 (MQ=2)


                                                                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccgg      <  2:577947/101‑1 (MQ=2)


                                                                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.CGCGACCCCCTGCCCcggcgg      >  1:4038341/1‑101 (MQ=17)


GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGG.G.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCG  >  NC_014034/2493904‑2494100

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG