Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,014 A→G 7.9% intergenic (+62/‑180) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0140A→G 7.9% 28.4 ‑2.5 ‑0.5176intergenic (+62/‑180)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (62/100):  total (71/105)
Fisher's exact test strand distribution p-value = 8.61e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.46e-01

Only 100 of 191 total aligned reads displayed.

AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113
                                                                                                      |                                                                                                          
aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                            >  1:543753/1‑101 (MQ=21)
 gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                           <  2:2526510/101‑1 (MQ=21)
  cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                          <  2:4360332/101‑1 (MQ=21)
  cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                          <  1:2709425/101‑1 (MQ=21)
      gcggcgGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                     <  2:2164660/101‑1 (MQ=21)
      gcggcgGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                     <  2:5533574/101‑1 (MQ=21)
       cggcggGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATg                                                                                                    <  2:3747437/101‑1 (MQ=21)
          cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCt                                                                                                 >  1:3114725/1‑101 (MQ=21)
          cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCt                                                                                                 <  1:5606574/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                <  2:5331538/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                <  1:3340066/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                <  2:4272241/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                <  1:195317/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCCGGACATTTGCAGCCAGATGAAAACGG.AGGccgt                                                                                                >  1:5716720/1‑99 (MQ=18)
            gggggggggCCCGCCAGGCCGAGTAAG.GGGGGGG.CGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                               <  1:3210721/94‑1 (MQ=17)
            ggcgTGCGGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                               >  1:5239086/1‑101 (MQ=18)
             gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                               <  2:4441272/101‑1 (MQ=18)
              cgcgcgGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCa                                                                                             >  1:1526278/1‑101 (MQ=21)
               gcgcgGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                            <  1:5128591/101‑1 (MQ=21)
               ccgcgGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                            <  2:3005454/100‑1 (MQ=18)
                cgcgGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATc                                                                                           <  2:2335079/101‑1 (MQ=21)
                 gcgGCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCt                                                                                          <  2:3431272/101‑1 (MQ=21)
                   ggCACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTg                                                                                        <  1:4043740/101‑1 (MQ=21)
                     cACGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGcc                                                                                      >  2:5366437/1‑101 (MQ=21)
                      aCGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                     >  1:2371653/1‑101 (MQ=21)
                      aCGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                     <  1:2862297/101‑1 (MQ=21)
                      aCGACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGGCCGCCAGCGGACTCCCCCCCGGATATTTGCCGCAAGAAGAAAACGGAAGGCCTTTCTTCtgccc                                                                                      >  1:1041465/1‑97 (MQ=9)
                        gACAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCCG.ATGCCTTTCATCTTGgccca                                                                                   >  1:2620391/1‑99 (MQ=18)
                         aCAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaa                                                                                  <  2:5258250/101‑1 (MQ=21)
                          cAGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                 <  2:3984427/101‑1 (MQ=21)
                           aGGCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                <  2:4539795/101‑1 (MQ=21)
                             gCCGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                              <  1:3597734/101‑1 (MQ=21)
                             gCCGAGTAA..GGGGGGGCCGCTGCCCCCCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                              <  2:5644917/101‑1 (MQ=21)
                              ccGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                             <  2:329755/101‑1 (MQ=21)
                              ccGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                             <  2:5480580/101‑1 (MQ=21)
                               cGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                            >  1:1610409/1‑101 (MQ=18)
                               cGAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                            <  1:1768512/101‑1 (MQ=21)
                                gAGTAAG.GGGGGGG.CGGTGCCCCCCGTCCGCAAGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                           <  2:4730446/101‑1 (MQ=18)
                                gAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                           >  1:1109696/1‑101 (MQ=18)
                                gAGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                           >  1:5668494/1‑101 (MQ=18)
                                 aGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCCAGCGGACTCCCACCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                          <  2:2268504/101‑1 (MQ=18)
                                 aGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                          >  1:1711471/1‑101 (MQ=18)
                                 aGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                          <  2:410552/101‑1 (MQ=18)
                                 aGTAAG.GGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                          <  2:1405575/101‑1 (MQ=18)
                                   gtaagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                         >  2:5087086/6‑101 (MQ=255)
                                     aagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                       >  2:190980/4‑101 (MQ=255)
                                      agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                      <  1:2298560/99‑1 (MQ=255)
                                      agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCGGGGTTTTTGCCGCCAGCTGACACCGG.ATGCCCTTCATCTTCCCCTAAATAATCCggggg                                                                      >  2:2288534/3‑101 (MQ=12)
                                       gggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                     <  1:4207758/100‑1 (MQ=255)
                                         gggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                   <  1:5728365/101‑1 (MQ=255)
                                          gggcg.CGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                  <  2:5703803/101‑1 (MQ=18)
                                           gggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                 >  2:2477211/1‑101 (MQ=255)
                                            ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                               <  1:1688307/101‑1 (MQ=255)
                                            ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                               <  1:679154/101‑1 (MQ=255)
                                              g.cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.Tgg                                                             >  1:1959831/1‑101 (MQ=255)
                                                cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                            >  2:3060121/1‑101 (MQ=255)
                                                 gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                         >  2:3684816/1‑101 (MQ=255)
                                                   tGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGCAAGATGCAACCGG.GTGCCTTTCCTCTTTTCCCAAATAACCCGGGGGGGGacaggggaa                                                          >  2:4961732/1‑93 (MQ=12)
                                                   tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                      >  1:5283323/1‑100 (MQ=255)
                                                    gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                     <  2:4908975/101‑3 (MQ=255)
                                                    gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                     <  1:4336343/101‑3 (MQ=255)
                                                    gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                     >  1:2070495/1‑99 (MQ=255)
                                                     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                   <  1:1249727/101‑5 (MQ=255)
                                                     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                   <  2:3399449/101‑5 (MQ=255)
                                                      ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                  <  1:4722280/101‑6 (MQ=255)
                                                      ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ACGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGCC..G.aagaca                                                  >  2:883429/1‑96 (MQ=18)
                                                        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                 <  1:4838278/97‑7 (MQ=255)
                                                         ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACAGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccaga                                                >  2:918705/4‑94 (MQ=18)
                                                          cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                               <  1:2977745/99‑9 (MQ=255)
                                                           gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                              <  2:1256930/100‑10 (MQ=255)
                                                                 aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                                         <  2:1919512/101‑1 (MQ=18)
                                                                   gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                       >  2:3927288/1‑99 (MQ=18)
                                                                   gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                       >  1:101779/1‑99 (MQ=18)
                                                                    cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                      >  2:2783474/1‑98 (MQ=18)
                                                                      gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                                  >  2:596877/1‑101 (MQ=18)
                                                                       aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.c                                >  1:2841308/1‑101 (MQ=18)
                                                                          ccccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.cccc                             >  2:2990638/1‑100 (MQ=17)
                                                                            ccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.cccctg                           <  1:1153654/101‑4 (MQ=17)
                                                                               cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGccc                         >  1:3401375/1‑101 (MQ=17)
                                                                               cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGccc                         <  1:3748748/101‑1 (MQ=17)
                                                                               cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGccc                         <  1:2517749/101‑1 (MQ=17)
                                                                                gggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGcccc                        >  2:5233772/1‑101 (MQ=0)
                                                                                 ggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                       <  1:1558387/101‑1 (MQ=0)
                                                                                 ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGAGC.AGCGCCC.CCCTGCCCcg                       >  1:3456871/1‑101 (MQ=17)
                                                                                  gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                      >  2:4053107/1‑100 (MQ=0)
                                                                                  gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                      >  2:1503599/1‑100 (MQ=2)
                                                                                  gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                      >  1:2232856/1‑100 (MQ=2)
                                                                                  gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                      >  1:5353248/1‑101 (MQ=18)
                                                                                  gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                      >  1:5398684/1‑101 (MQ=17)
                                                                                   atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                     >  1:97697/1‑99 (MQ=2)
                                                                                   atatTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                     >  2:1738181/1‑101 (MQ=17)
                                                                                     atTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                   <  2:577947/101‑1 (MQ=2)
                                                                                     atTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.CGCGACC.CCCTGCCCcggcgg                   >  1:4038341/1‑101 (MQ=17)
                                                                                       ttGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                 <  2:3238589/101‑3 (MQ=2)
                                                                                         gcagcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGGGGGCCg               >  1:4383610/1‑101 (MQ=14)
                                                                                          cagcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.CCACCCTGCCCCGGCGGTCCgg              >  2:1786314/1‑100 (MQ=18)
                                                                                             caAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc           >  2:1579395/1‑101 (MQ=17)
                                                                                               aGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg         >  2:4204943/1‑101 (MQ=18)
                                                                                                    aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt    <  2:101759/101‑1 (MQ=17)
                                                                                                      aCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGCCGGCGCCTTg  >  2:1191765/1‑101 (MQ=17)
                                                                                                      |                                                                                                          
AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG