Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,015 C→G 7.9% intergenic (+63/‑179) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0150C→G 7.9% 27.9 ‑2.3 ‑0.1177intergenic (+63/‑179)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (66/97):  total (75/102)
Fisher's exact test strand distribution p-value = 9.72e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.80e-01

Only 100 of 193 total aligned reads displayed.

GCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGA  >  NC_014034/2493916‑2494114
                                                                                                     |                                                                                                          
gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                            <  2:2526510/101‑1 (MQ=21)
 cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                           <  1:553261/101‑1 (MQ=21)
  gccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                          >  2:5668893/1‑101 (MQ=21)
   ccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                         >  1:5308800/1‑101 (MQ=21)
     gcggcgGGCGCGCGGCACGCCAGGCCGAGTCAG.GGGGGGGCGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                      <  1:369795/101‑1 (MQ=18)
     gcggcgGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.At                                                                                                      <  2:2164660/101‑1 (MQ=21)
      gggggggcgCCGGGCCCGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGc                                                                                                    <  1:2269614/97‑1 (MQ=18)
      cggcggGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATg                                                                                                     <  2:3747437/101‑1 (MQ=21)
         cgggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCt                                                                                                  >  1:3114725/1‑101 (MQ=21)
          gggcgGGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTGCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                 <  2:1908503/101‑1 (MQ=18)
          gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                 <  1:195317/101‑1 (MQ=21)
          gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCtt                                                                                                 <  1:3340066/101‑1 (MQ=21)
          gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCCGGACATTTGCAGCCAGATGAAAACGG.AGGccgt                                                                                                 >  1:5716720/1‑99 (MQ=18)
           gggggggggCCCGCCAGGCCGAGT.AAGGGGGGGGCGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                <  1:3210721/94‑1 (MQ=17)
           ggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCttt                                                                                                <  1:404052/101‑1 (MQ=21)
             cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCa                                                                                              >  1:1526278/1‑101 (MQ=21)
              gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                             <  1:5128591/101‑1 (MQ=21)
              gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCAt                                                                                             <  2:5376790/101‑1 (MQ=21)
               cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATc                                                                                            <  2:2335079/101‑1 (MQ=21)
                gcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCt                                                                                           <  2:3431272/101‑1 (MQ=21)
                 cgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCtt                                                                                          <  2:4126816/101‑1 (MQ=21)
                     aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGccc                                                                                      <  2:4743496/101‑1 (MQ=21)
                     aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGGCCGCCAGCGGACTCCCCCCCGGATATTTGCCGCAAGAAGAAAACGGAAGGCCTTTCTTCtgccc                                                                                       >  1:1041465/1‑97 (MQ=9)
                         cAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                  <  2:3984427/101‑1 (MQ=21)
                         cAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGaaa                                                                                  <  1:2331066/101‑1 (MQ=21)
                          aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                 <  2:4629544/101‑1 (MQ=21)
                          aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                 >  1:270633/1‑101 (MQ=21)
                          aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAat                                                                                 >  2:3411163/1‑101 (MQ=21)
                           ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAata                                                                                >  1:4318997/1‑101 (MQ=21)
                            gCCGAGT.AAGGGGGGGGCGTTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                               <  2:438697/101‑1 (MQ=18)
                            gCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                               <  1:3597734/101‑1 (MQ=21)
                             gccgggtagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAatat                                                                               <  1:290385/91‑1 (MQ=18)
                             ccGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATc                                                                              >  2:5270971/1‑101 (MQ=18)
                              cGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                             <  1:1768512/101‑1 (MQ=21)
                              cGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATcc                                                                             >  1:1610409/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                            >  2:5357442/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATccc                                                                            >  1:1109696/1‑101 (MQ=18)
                                aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCCAGCGGACTCCCACCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                           <  2:2268504/101‑1 (MQ=18)
                                aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                           <  2:1405575/101‑1 (MQ=18)
                                aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                           >  1:1711471/1‑101 (MQ=18)
                                 ggaagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCg                                                                           <  2:3377528/95‑1 (MQ=18)
                                  gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                          <  2:4477155/96‑1 (MQ=255)
                                  gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                          >  2:5087086/6‑101 (MQ=255)
                                     aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                       >  2:3237842/3‑101 (MQ=255)
                                     aggggggggCGCTGCCCCCCGTCCGCAAGCGGACCCCCCCCGGGGTTTTTGCCGCCAGCTGACACCGG.ATGCCCTTCATCTTCCCCTAAATAATCCggggg                                                                       >  2:2288534/3‑101 (MQ=12)
                                       gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                     <  2:4936145/101‑1 (MQ=255)
                                        ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                    <  1:5728365/101‑1 (MQ=255)
                                          ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                  >  2:2477211/1‑101 (MQ=255)
                                          ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                  <  1:4076109/101‑1 (MQ=255)
                                           gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                <  1:1688307/101‑1 (MQ=255)
                                           gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                <  1:679154/101‑1 (MQ=255)
                                             gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.Tgg                                                              >  1:1959831/1‑101 (MQ=255)
                                               gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                          >  2:3684816/1‑101 (MQ=255)
                                               gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                          <  2:5573735/101‑1 (MQ=255)
                                                cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                        <  1:536070/101‑1 (MQ=255)
                                                 tGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGCAAGATGCAACCGG.GTGCCTTTCCTCTTTTCCCAAATAACCCGGGGGGGGacaggggaa                                                           >  2:4961732/1‑93 (MQ=12)
                                                  gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                      >  1:2070495/1‑99 (MQ=255)
                                                   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                    <  1:1249727/101‑5 (MQ=255)
                                                   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..G.aagcc                                                    >  2:1913058/1‑97 (MQ=18)
                                                    ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                   <  1:4722280/101‑6 (MQ=255)
                                                    ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ACGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGCC..G.aagaca                                                   >  2:883429/1‑96 (MQ=18)
                                                      cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                  <  1:4838278/97‑7 (MQ=255)
                                                       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                 <  2:4252111/98‑8 (MQ=255)
                                                       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                 >  2:628593/4‑94 (MQ=255)
                                                       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACAGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccaga                                                 >  2:918705/4‑94 (MQ=18)
                                                         gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                               <  2:1256930/100‑10 (MQ=255)
                                                         gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                               <  2:431362/100‑10 (MQ=255)
                                                          tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                              <  1:2566573/101‑11 (MQ=255)
                                                               aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                                          <  2:1919512/101‑1 (MQ=18)
                                                                 gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                        >  2:3927288/1‑99 (MQ=18)
                                                                 gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                        >  1:101779/1‑99 (MQ=18)
                                                                  cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                       >  2:2783474/1‑98 (MQ=18)
                                                                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc.c                                >  1:2722749/1‑101 (MQ=18)
                                                                       tCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc.cc                               >  2:593976/1‑101 (MQ=18)
                                                                        ccccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc.ccc                              >  2:2990638/1‑100 (MQ=17)
                                                                          ccccGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc.ccctg                            <  1:1153654/101‑4 (MQ=17)
                                                                             cGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGccc                          >  1:3401375/1‑101 (MQ=17)
                                                                              gggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGcccc                         >  2:5233772/1‑101 (MQ=0)
                                                                               ggATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcg                        <  1:1558387/101‑1 (MQ=0)
                                                                               ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCcg                        <  2:5706753/101‑1 (MQ=17)
                                                                               ggATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGAGC.AGCGCCCC.CCTGCCCcg                        >  1:3456871/1‑101 (MQ=17)
                                                                                gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                       >  2:4053107/1‑100 (MQ=0)
                                                                                gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                       >  1:2232856/1‑100 (MQ=2)
                                                                                gATATTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgc                       >  2:1503599/1‑100 (MQ=2)
                                                                                gATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAACCAGAGGGGGGC.AGCGCCCC.CCTGCCCcgg                       >  1:5262642/1‑101 (MQ=17)
                                                                                 atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgcc                      >  1:97697/1‑99 (MQ=2)
                                                                                 atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgcc                      >  1:2461050/1‑99 (MQ=2)
                                                                                 atatTTGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgcc                      <  2:3021862/101‑3 (MQ=2)
                                                                                 atatTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCcggc                      >  2:1738181/1‑101 (MQ=17)
                                                                                 atatTGGCGGCAAGATGAAGGGGC.ATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCC.CCTGCCCcgcc                      <  1:5657283/101‑3 (MQ=2)
                                                                                      tGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                 <  2:5492465/101‑1 (MQ=17)
                                                                                       gcagcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGGGGGCCg                >  1:4383610/1‑101 (MQ=14)
                                                                                          gcaAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg             >  2:329376/1‑101 (MQ=18)
                                                                                           caAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc            >  2:1579395/1‑101 (MQ=17)
                                                                                             aGATGAAACCGG.ATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTGAA.TGGA..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg          <  1:2846712/101‑1 (MQ=9)
                                                                                                  aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt     >  1:5411536/1‑101 (MQ=18)
                                                                                                  aaaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt     <  2:101759/101‑1 (MQ=17)
                                                                                                   aaCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCtt    >  1:3949650/1‑101 (MQ=18)
                                                                                                    aCCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGCCGGCGCCTTg   >  2:1191765/1‑101 (MQ=17)
                                                                                                     ccGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAGCCCGAGGGGGGC.AGCG.CCCGCCTGCCCCGGCGGCCCGGCGCGGCGCCTGGa  >  1:5192999/1‑101 (MQ=9)
                                                                                                     |                                                                                                          
GCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGG.ATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGA  >  NC_014034/2493916‑2494114

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG