Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,061 G→A 10.5% intergenic (+109/‑133) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0610G→A10.5% 32.5 ‑0.3 ‑0.9133intergenic (+109/‑133)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/5):  ref base (67/52):  total (76/57)
Fisher's exact test strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.15e-01

Only 100 of 144 total aligned reads displayed.

GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493961‑2494151
                                                                                                        |                                                                                              
gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..g                                                                                                <  2:5573735/101‑1 (MQ=255)
 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.a                                                                                              <  1:536070/101‑1 (MQ=255)
  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aa                                                                                             <  2:3987542/101‑2 (MQ=255)
  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGC..G.aa                                                                                             >  1:5283323/1‑100 (MQ=255)
   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGC..G.aac                                                                                            >  1:5799507/1‑99 (MQ=18)
   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aag                                                                                            <  1:4336343/101‑3 (MQ=255)
   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aag                                                                                            <  2:4908975/101‑3 (MQ=255)
    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagcc                                                                                          <  2:3399449/101‑5 (MQ=255)
    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagcc                                                                                          <  1:1249727/101‑5 (MQ=255)
     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagcca                                                                                         <  1:4722280/101‑6 (MQ=255)
     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGCC..G.aagaca                                                                                         >  2:883429/1‑96 (MQ=18)
       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccag                                                                                        <  1:4838278/97‑7 (MQ=255)
        ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccaga                                                                                       <  2:4252111/98‑8 (MQ=255)
        ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACAGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGC..G.aagccaga                                                                                       >  2:918705/4‑94 (MQ=18)
         cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccagag                                                                                      <  1:900261/99‑9 (MQ=255)
         cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccagag                                                                                      <  1:2977745/99‑9 (MQ=255)
          gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccagagg                                                                                     <  2:1256930/100‑10 (MQ=255)
          gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccagagg                                                                                     <  2:431362/100‑10 (MQ=255)
           tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.aagccagaggg                                                                                    >  1:1692751/1‑91 (MQ=255)
                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCa                                                                               <  2:1919512/101‑1 (MQ=18)
                  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                             >  2:3927288/1‑99 (MQ=18)
                  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                             >  1:101779/1‑99 (MQ=18)
                   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                            >  2:2783474/1‑98 (MQ=18)
                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                           >  1:2080257/1‑97 (MQ=18)
                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                        >  2:596877/1‑101 (MQ=18)
                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                      >  1:2841308/1‑101 (MQ=18)
                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc                                                                     >  1:2722749/1‑101 (MQ=18)
                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cc.ccc                                                                    <  2:2306639/101‑1 (MQ=17)
                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.ccc                                                                    >  2:593976/1‑101 (MQ=18)
                         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                                   >  2:1216098/1‑100 (MQ=18)
                            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctgc                                                                <  2:1085111/101‑5 (MQ=17)
                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                               <  1:2517749/101‑1 (MQ=17)
                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                               >  1:3401375/1‑101 (MQ=17)
                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                               <  1:3748748/101‑1 (MQ=17)
                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                             <  1:1558387/101‑1 (MQ=0)
                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                             <  2:5706753/101‑1 (MQ=17)
                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                            >  2:4053107/1‑100 (MQ=0)
                                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                            >  1:5398684/1‑101 (MQ=17)
                                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAA.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                            >  1:5262642/1‑101 (MQ=17)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           <  2:3021862/101‑3 (MQ=2)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  2:1511896/1‑99 (MQ=2)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  1:2461050/1‑99 (MQ=2)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  1:97697/1‑99 (MQ=2)
                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                                                           >  2:2837301/1‑101 (MQ=17)
                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                                                           >  2:1738181/1‑101 (MQ=17)
                                  atatTGGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           <  1:5657283/101‑3 (MQ=2)
                                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccg                                                          >  2:254360/1‑101 (MQ=2)
                                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTG.AATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                       <  2:3238589/101‑3 (MQ=2)
                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGGGGGCCg                                                     >  1:4383610/1‑101 (MQ=14)
                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCACCCTGCCCCGGCGGTCCgg                                                    >  2:1786314/1‑100 (MQ=18)
                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                                  >  2:329376/1‑101 (MQ=18)
                                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                 >  2:1579395/1‑101 (MQ=17)
                                              aGATGAAACCGGATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTG.AA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                               <  1:2846712/101‑1 (MQ=9)
                                                    acccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCtt                                         <  1:1954441/99‑1 (MQ=17)
                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                         >  1:3949650/1‑101 (MQ=18)
                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                         >  1:5728138/1‑101 (MQ=18)
                                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                       >  2:746152/1‑101 (MQ=18)
                                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                    >  2:342131/1‑101 (MQ=18)
                                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTgcgggg                                   >  1:110544/1‑96 (MQ=17)
                                                           tcccTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  <  2:1154967/99‑1 (MQ=17)
                                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  >  1:5365492/1‑101 (MQ=18)
                                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  <  2:5283033/101‑1 (MQ=17)
                                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGGCCGTGGCGGGGCCTTGAcggcgg                                 >  1:1075048/1‑98 (MQ=14)
                                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                <  2:5245935/101‑1 (MQ=17)
                                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:138099/1‑101 (MQ=17)
                                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:3962078/1‑101 (MQ=18)
                                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:2635853/1‑101 (MQ=18)
                                                               tttCTTCTTGCCCGAAATATCGCGGGGGGTG.AA.GGGC..GAAG.CGAGAGGGGGGCAGCCC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  1:4956912/101‑1 (MQ=21)
                                                               tttCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              >  2:1404975/1‑101 (MQ=18)
                                                               tttCATCTGGCCCGAAACATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  2:5191648/101‑1 (MQ=17)
                                                                ttCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGGggggg                             >  1:2046882/1‑101 (MQ=17)
                                                                 tCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGT..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                            >  2:390890/1‑100 (MQ=18)
                                                                 tCATCTTGCCCGAAATAGCCCGGGGGGTG.AA.TGGC..GAAG.CCGGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                            <  1:3956981/101‑1 (MQ=18)
                                                                    tCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGGcgcg                         >  1:4266567/1‑101 (MQ=18)
                                                                     cTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCCCCTTGACGGGCGGGGGCGCgg                        <  1:5455301/101‑1 (MQ=18)
                                                                      ttGCCCGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCcgg                       >  2:2503471/1‑101 (MQ=18)
                                                                       tGCCCGAAATATCCCGGGGGGGG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgcacgc                      >  2:5426171/1‑97 (MQ=18)
                                                                          ccGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                   <  2:5414592/101‑1 (MQ=18)
                                                                          ccGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                   <  2:5639062/101‑1 (MQ=18)
                                                                           cGAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                  <  1:191000/101‑1 (MQ=18)
                                                                            gAAATATGCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.TC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                 <  1:2674986/101‑1 (MQ=21)
                                                                            gAAATATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                 >  2:2183950/1‑101 (MQ=18)
                                                                            gAAATATCCCGGGGGGTG.AA.TGGC..G.AA.GCCAGAGGGGGC.AGCGCCC.CCCCGCCCCCGCGGGCCGTGGCCGCGGCCTGACCGGGGGGGGCGCggggccttac                >  2:1157928/1‑94 (MQ=0)
                                                                              aaTATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc               >  1:215147/1‑101 (MQ=21)
                                                                              aaTATCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCACGGCCTTGACGGGCGGGGGCGCGGGCATTaaac               >  1:4992312/1‑99 (MQ=17)
                                                                               atatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg              >  1:4725475/1‑101 (MQ=21)
                                                                               atatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg              >  2:5222576/1‑101 (MQ=21)
                                                                               atatCCCGGGGGGGGAAA.GGGC..A.AG.CCAAAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg              <  2:1644352/101‑1 (MQ=25)
                                                                                tatCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             <  1:4430595/101‑1 (MQ=21)
                                                                                  tCCCGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa           <  1:3685044/101‑1 (MQ=21)
                                                                                   cccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGAATTAACCGAAAt          <  1:3137359/101‑1 (MQ=21)
                                                                                   cccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGACGGGCGGGGGCGCGGGCATTAACCGAAAt          <  1:5126909/101‑1 (MQ=21)
                                                                                    ccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt         >  2:4893213/1‑101 (MQ=21)
                                                                                    ccGGGGGGTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt         >  2:3832046/1‑101 (MQ=21)
                                                                                     cGGGGGGTG.AA.TGGC..GAAG.CAAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg        <  2:1404434/101‑1 (MQ=25)
                                                                                      ggggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCGTTAACCGAAATTga       >  1:447796/1‑100 (MQ=21)
                                                                                      ggggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc       <  2:4826904/101‑1 (MQ=25)
                                                                                       gggggTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  2:636029/1‑101 (MQ=25)
                                                                                        ggggTG.AA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  2:3075194/1‑101 (MQ=25)
                                                                                           gTG.AA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  <  1:341077/101‑1 (MQ=25)
                                                                                                        |                                                                                              
GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTG.AA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493961‑2494151

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG