Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,064 C→G 13.2% intergenic (+112/‑130) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0640C→G13.2% 33.4 ‑0.2 ‑0.9114intergenic (+112/‑130)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/5):  ref base (61/38):  total (71/43)
Fisher's exact test strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.32e-01

Only 100 of 120 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493977‑2494151
                                                                                            |                                                                                         
aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                                               <  2:1919512/101‑1 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                             >  1:101779/1‑99 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                             >  2:3927288/1‑99 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                            >  2:2783474/1‑98 (MQ=18)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                        >  2:596877/1‑101 (MQ=18)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                      >  1:2841308/1‑101 (MQ=18)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc                                                                     >  1:2722749/1‑101 (MQ=18)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cc.ccc                                                                    <  2:2306639/101‑1 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.ccc                                                                    >  2:593976/1‑101 (MQ=18)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                                   >  2:2990638/1‑100 (MQ=17)
         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                                   >  2:1216098/1‑100 (MQ=18)
           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                 <  1:1153654/101‑4 (MQ=17)
            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctgc                                                                <  2:1085111/101‑5 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                               >  1:3401375/1‑101 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                               <  1:2517749/101‑1 (MQ=17)
               gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                              >  2:5233772/1‑101 (MQ=0)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                             <  1:1558387/101‑1 (MQ=0)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                             <  2:5706753/101‑1 (MQ=17)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGAGC.AGCGCCC.CCCTGCCCcg                                                             >  1:3456871/1‑101 (MQ=17)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                            >  1:2232856/1‑100 (MQ=2)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                            >  2:1503599/1‑100 (MQ=2)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                            >  2:4053107/1‑100 (MQ=0)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                            >  1:5398684/1‑101 (MQ=17)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                            >  1:5353248/1‑101 (MQ=18)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAA.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                            >  1:5262642/1‑101 (MQ=17)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  2:1511896/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  1:2461050/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           >  1:97697/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           <  2:3021862/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                                                           >  2:1738181/1‑101 (MQ=17)
                  atatTGGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                           <  1:5657283/101‑3 (MQ=2)
                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccg                                                          >  2:254360/1‑101 (MQ=2)
                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.CGCGACC.CCCTGCCCcggcgg                                                         >  1:4038341/1‑101 (MQ=17)
                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                       <  2:3238589/101‑3 (MQ=2)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                      <  2:5492465/101‑1 (MQ=17)
                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGGGGGCCg                                                     >  1:4383610/1‑101 (MQ=14)
                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCACCCTGCCCCGGCGGTCCgg                                                    >  2:1786314/1‑100 (MQ=18)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                 >  1:3195975/1‑101 (MQ=18)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                 >  2:1579395/1‑101 (MQ=17)
                              aGATGAAACCGGATTCCGTTCATCTTGCCTGAAATATTCCGGGGGGTGAA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                               <  1:2846712/101‑1 (MQ=9)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                               >  2:4204943/1‑101 (MQ=18)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                          >  1:5411536/1‑101 (MQ=18)
                                    acccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCtt                                         <  1:1954441/99‑1 (MQ=17)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                         >  1:5728138/1‑101 (MQ=18)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                         >  1:3949650/1‑101 (MQ=18)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGCCGGCGCCTTg                                        >  2:1191765/1‑101 (MQ=17)
                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                       >  2:746152/1‑101 (MQ=18)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                      >  1:745251/1‑101 (MQ=18)
                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                     >  2:3084474/1‑101 (MQ=17)
                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                    >  2:342131/1‑101 (MQ=18)
                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTgcgggg                                   >  1:110544/1‑96 (MQ=17)
                                           tcccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  <  2:1154967/99‑1 (MQ=17)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  >  1:5365492/1‑101 (MQ=18)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  <  2:5283033/101‑1 (MQ=17)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  >  2:2526353/1‑101 (MQ=18)
                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGGCCGTGGCGGGGCCTTGAcggcgg                                 >  1:1075048/1‑98 (MQ=14)
                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                <  2:5245935/101‑1 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:138099/1‑101 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:3962078/1‑101 (MQ=18)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:2635853/1‑101 (MQ=18)
                                               tttCTTCTTGCCCGAAATATCGCGGGGGGTGAA.GGGC..GAAG.CGAGAGGGGGGCAGCCC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  1:4956912/101‑1 (MQ=21)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              >  2:1404975/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  1:5669188/101‑1 (MQ=17)
                                               tttCATCTGGCCCGAAACATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  2:5191648/101‑1 (MQ=17)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGGggggg                             >  1:2046882/1‑101 (MQ=17)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGT..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                            >  2:390890/1‑100 (MQ=18)
                                                 tCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCGGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                            <  1:3956981/101‑1 (MQ=18)
                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                         >  2:786796/1‑101 (MQ=18)
                                                      ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCcgg                       >  2:2503471/1‑101 (MQ=18)
                                                       tGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgcacgc                      >  2:5426171/1‑97 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                   <  2:5414592/101‑1 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                   <  2:5639062/101‑1 (MQ=18)
                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                  <  1:191000/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                 >  2:2183950/1‑101 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGC.AGCGCCC.CCCCGCCCCCGCGGGCCGTGGCCGCGGCCTGACCGGGGGGGGCGCggggccttac                >  2:1157928/1‑94 (MQ=0)
                                                             aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                <  2:4504181/101‑1 (MQ=18)
                                                              aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc               >  1:215147/1‑101 (MQ=21)
                                                              aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCACGGCCTTGACGGGCGGGGGCGCGGGCATTaaac               >  1:4992312/1‑99 (MQ=17)
                                                               atatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg              >  2:5222576/1‑101 (MQ=21)
                                                               atatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGGCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTTACCg              >  1:4980211/1‑101 (MQ=18)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             <  1:4430595/101‑1 (MQ=21)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             <  2:1684934/101‑1 (MQ=21)
                                                                 atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGCGGGGGGGGGGGCGGGGATTAACCGaa            >  1:3943106/1‑101 (MQ=12)
                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa           <  1:3685044/101‑1 (MQ=21)
                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCTCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt          <  1:4381020/101‑1 (MQ=21)
                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGACGGGCGGGGGCGCGGGCATTAACCGAAAt          <  1:5126909/101‑1 (MQ=21)
                                                                    ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt         >  2:3832046/1‑101 (MQ=21)
                                                                    ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt         >  2:4893213/1‑101 (MQ=21)
                                                                    ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt         >  1:4447184/1‑101 (MQ=21)
                                                                     cGGGGGGTGAA.TGGC..GAAG.CAAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg        <  2:1404434/101‑1 (MQ=25)
                                                                      ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCGTTAACCGAAATTga       >  1:447796/1‑100 (MQ=21)
                                                                      ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc       <  2:4826904/101‑1 (MQ=25)
                                                                      ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc       >  1:2924840/1‑101 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  2:3301323/1‑101 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  2:636029/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  2:3075194/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     <  1:2698124/101‑1 (MQ=25)
                                                                          ggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa   <  1:189655/101‑1 (MQ=25)
                                                                           gTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  <  1:4768207/101‑1 (MQ=25)
                                                                           gTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  <  1:341077/101‑1 (MQ=25)
                                                                                            |                                                                                         
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493977‑2494151

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG