Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,523,952 T→G 100% I573S (ATC→AGC)  ppiD → peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,523,9520T→G100.0% 1067.4 278I573S (ATC→AGC) ppiDpeptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  new base (140/138):  ref base (0/0):  total (140/138)

Only 100 of 282 total aligned reads displayed.

CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049
                                                                                                  |                                                                                                 
 aaCAAGCTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCa                                                                                                <  1:1503909/100‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               >  1:5591200/1‑101 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               <  1:1304183/101‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               >  1:3466910/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              <  2:4082338/101‑1 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              >  2:4666763/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              <  2:3804998/101‑1 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             >  2:329121/1‑101 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             >  2:5615267/1‑101 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            >  2:77956/1‑101 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            <  2:2807769/101‑1 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTAAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            <  2:1810218/101‑1 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           >  1:60669/1‑101 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           >  1:2066906/1‑101 (MQ=255)
       gTCTTTGTCGTCACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCt                                                                                          <  2:3113838/101‑1 (MQ=255)
       gTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCt                                                                                          >  2:4161047/1‑101 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         <  2:3021761/101‑1 (MQ=255)
         cTTTGTTGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCGAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        >  1:4932098/1‑101 (MQ=255)
          tttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       >  2:1144270/1‑101 (MQ=255)
              tcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGcc                                                                                   <  2:5590908/101‑1 (MQ=255)
              tcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGcc                                                                                   <  1:4791998/101‑1 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  2:5041221/1‑101 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  2:600000/1‑101 (MQ=255)
                    ccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             >  1:4036665/1‑101 (MQ=255)
                    ccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  1:329165/101‑1 (MQ=255)
                    ccGGGCGCGCGGTGAACCCGGCCGCTCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  2:1956616/101‑1 (MQ=255)
                     cGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTgg                                                                            >  1:3662002/1‑101 (MQ=255)
                     cGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTgg                                                                            >  1:5458492/1‑101 (MQ=255)
                     cGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTgg                                                                            <  1:4458369/101‑1 (MQ=255)
                      gTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggc                                                                           <  2:4854135/101‑1 (MQ=255)
                      gTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggc                                                                           >  1:1714156/1‑101 (MQ=255)
                       tGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcg                                                                          <  1:4961865/101‑1 (MQ=255)
                         cgcgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcggc                                                                        >  1:2348309/1‑101 (MQ=255)
                          gcgcgGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGcc                                                                       <  1:1984910/101‑1 (MQ=255)
                           cggggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCt                                                                      <  1:2287643/98‑1 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     >  1:3065133/1‑101 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     <  2:1192708/101‑1 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGATGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGACGGCCtt                                                                     >  2:3243121/1‑101 (MQ=255)
                              ggTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  1:4627229/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  1:4372357/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  2:4971498/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  2:2192304/101‑1 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  >  1:4870328/1‑101 (MQ=255)
                                tGAACCCGTCCGATCCGGCCGATCCCGAGCTGGCGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:4999107/101‑1 (MQ=255)
                                      cggccgatccggccgatcCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGt                                                           <  2:2067487/101‑1 (MQ=255)
                                       ggccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTg                                                          <  2:4684604/101‑1 (MQ=255)
                                        gccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGc                                                         <  2:995626/101‑1 (MQ=255)
                                        gccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGc                                                         <  1:1392064/101‑1 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgcg                                                     <  1:35879/101‑1 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgcc                                                     >  2:3404218/1‑100 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGCTGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  >  1:850607/1‑101 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  2:5742360/101‑1 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  2:1531804/101‑1 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  >  1:2508116/1‑101 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  1:3182070/101‑1 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  2:2446533/101‑1 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 >  1:2180012/1‑101 (MQ=255)
                                                 gccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggc                                                <  2:5439541/101‑1 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgg                                             >  2:1697106/1‑100 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  1:3794120/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            >  2:4384336/1‑101 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          >  1:333880/1‑101 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          >  2:44642/1‑101 (MQ=255)
                                                         cGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggccg                                        >  1:994357/1‑101 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  1:5750013/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  2:2983749/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   <  1:3363010/101‑1 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  1:4682425/1‑101 (MQ=255)
                                                                gTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAc                                 >  2:5211019/1‑101 (MQ=255)
                                                                gTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAc                                 <  1:2297540/101‑1 (MQ=255)
                                                                 tGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAcc                                <  1:3562430/101‑1 (MQ=255)
                                                                 tGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAcc                                >  1:2154277/1‑101 (MQ=255)
                                                                  gCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAccc                               >  1:4529579/1‑101 (MQ=255)
                                                                  gCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAccc                               <  2:4029441/101‑1 (MQ=255)
                                                                   cAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCt                              >  1:2948659/1‑101 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  1:431030/101‑1 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  2:3503115/101‑1 (MQ=255)
                                                                     gCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCg                            >  2:522209/1‑101 (MQ=255)
                                                                      cTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGa                           >  2:3853262/1‑101 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  2:3634015/101‑1 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  1:3864110/101‑1 (MQ=255)
                                                                        gcgcAAGGCCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  2:3566506/101‑1 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTCCGCCGGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  2:276356/101‑1 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  2:2154155/101‑1 (MQ=255)
                                                                         cgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAg                        >  1:1292790/1‑101 (MQ=255)
                                                                          gcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgc                       <  2:3661774/101‑1 (MQ=255)
                                                                          gcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgc                       >  1:5637444/1‑101 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    <  2:1440993/101‑1 (MQ=255)
                                                                                ccTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  2:2813722/101‑1 (MQ=255)
                                                                                    tGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCg             >  2:4753789/1‑101 (MQ=255)
                                                                                      acacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCt           >  2:689425/1‑101 (MQ=255)
                                                                                       cacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTg          <  1:107998/101‑1 (MQ=255)
                                                                                        acCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGc         >  2:3902532/1‑101 (MQ=255)
                                                                                         cccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGcc        <  1:1617135/101‑1 (MQ=255)
                                                                                         cccGGCTGAGCCAGATGCTCCGCGCCGATCTGGCGGCCTTCTACGCCCGCGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGcc        <  2:889212/101‑1 (MQ=255)
                                                                                            ggCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTg     <  2:1292707/101‑1 (MQ=255)
                                                                                             gCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGc    >  2:3341082/1‑101 (MQ=255)
                                                                                             gCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGc    <  1:4094094/101‑1 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   >  2:3967952/1‑101 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   >  1:3950741/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG