BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,555	C→T	100%	intergenic (+27/+167)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,555	0	C→T	100.0%	521.2	186	intergenic (+27/+167)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (47/138): ref base (0/0): total (48/138)

Only 100 of 201 total aligned reads displayed.


CTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.G.GGG.GCGCTGCCCCC.CTC.CT..G..C.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566456‑2566644


cTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGAAG.AAAC.GAGGG.GCGCTGCCCCc.ccc.c                                                                                                  >  2:3312138/1‑101 (MQ=255)


cTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGGTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAAC.G.GGG.GCGCGGTCCCc.ccc.c                                                                                                  >  2:3698971/1‑101 (MQ=255)


  aTGCGATGGTCGATGCGATCGCCTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..g                                                                                              <  1:1875668/101‑1 (MQ=255)


  aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..g                                                                                              <  2:5590406/101‑1 (MQ=255)


  aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGACCCC.CTC.TT..g                                                                                              <  1:4335753/101‑1 (MQ=255)


       aTGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..ggcctt                                                                                         <  1:4189479/101‑6 (MQ=38)


       aTGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..ggcctt                                                                                         <  1:3585313/101‑6 (MQ=38)


       aTGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..ggcctt                                                                                         <  2:3227032/101‑6 (MQ=38)


        tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..ggccttc                                                                                        <  2:5535036/101‑7 (MQ=38)


        tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.G.GGGTGCGCTGCCCCC.CTC.TT..ggccttc                                                                                        <  2:3301833/101‑7 (MQ=255)


           tCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..gggcttccgc                                                                                     >  2:5498222/1‑92 (MQ=255)


            cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.cc                                                                                  <  1:2947866/101‑1 (MQ=37)


               tGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAAt                                                                               <  2:677502/101‑1 (MQ=37)


                gcgatgtccTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGTCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAAtt                                                                              <  1:5501191/93‑1 (MQ=21)


                gCGATCGACTGCCCCGGGGGGTTCTGCCGCCGCGACATCGGCGGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAAtt                                                                              <  2:353185/101‑1 (MQ=25)


                 cGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTc                                                                             <  2:2206998/101‑1 (MQ=37)


                  gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AACCGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCa                                                                            <  2:862554/101‑1 (MQ=25)


                  gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCa                                                                            <  1:4639120/101‑1 (MQ=37)


                     cGACTGGCCCGGGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCGCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCAccc                                                                         <  1:1555670/101‑1 (MQ=25)


                     cGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCAccc                                                                         <  2:2854771/101‑1 (MQ=37)


                        cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCAcccccc                                                                      <  1:2375308/101‑1 (MQ=25)


                            cccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.a                                                                 <  1:1160426/101‑1 (MQ=25)


                               gAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCTCTGAGACGAAAAG.G.GGG.GCGCGGCCCCC.CTCTTT..G..CGTTCGG.GCACTTCACCCCCCgggcaat                                                               >  1:464977/1‑97 (MQ=255)


                               gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.atat                                                              <  1:739255/101‑1 (MQ=25)


                               gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.atat                                                              <  1:4816648/101‑1 (MQ=25)


                                 ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATAttt                                                            <  1:612127/101‑1 (MQ=21)


                                  gtgTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTc                                                           <  2:5745867/99‑1 (MQ=21)


                                  gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCAccccccccggcatttt                                                            >  1:4071215/1‑91 (MQ=21)


                                  gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTc                                                           <  2:1382023/101‑1 (MQ=21)


                                   ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCg                                                          <  2:3138014/101‑1 (MQ=21)


                                    gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGAAGAAAAG.G.GGG.GCGCTGCCCCC.CCC.TTTGG..C.TTCGGCCCAATCCCCCCCCCgggtcctttgg                                                           >  2:2061801/1‑93 (MQ=16)


                                      tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GGGCTGCCCCC.CCC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGc                                                       >  1:2831878/1‑101 (MQ=21)


                                      tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCgggg                                                       >  1:1413396/1‑100 (MQ=21)


                                       cTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCa                                                      <  2:1914657/101‑1 (MQ=21)


                                        tGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCaa                                                     <  1:3805170/101‑1 (MQ=21)


                                           cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCCATTCACCCCCCCGG.AGATTTCGGGCAAGaa                                                  >  2:1564995/1‑100 (MQ=21)


                                           cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGAt                                                  <  1:2123216/101‑1 (MQ=21)


                                           cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGAt                                                  <  1:1521637/101‑1 (MQ=21)


                                            gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATa                                                 <  2:5765050/101‑1 (MQ=21)


                                            gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATa                                                 <  2:1150900/101‑1 (MQ=21)


                                              cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATaaa                                               <  2:3673037/101‑1 (MQ=21)


                                                 gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAaagaa                                            <  1:5218538/101‑1 (MQ=21)


                                                  aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAaagaag                                           <  1:3420105/101‑1 (MQ=21)


                                                   cATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAgg                                          <  2:1941308/101‑1 (MQ=21)


                                                   cATCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAgg                                          <  1:1479045/101‑1 (MQ=21)


                                                     tCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTc                                        <  1:2857171/101‑1 (MQ=21)


                                                     tCGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTc                                        >  2:3358529/1‑101 (MQ=21)


                                                      cGGCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCt                                       <  1:3000140/101‑1 (MQ=21)


                                                       ggCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtt                                      <  1:485086/101‑1 (MQ=21)


                                                       ggCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtt                                      <  2:5469176/101‑1 (MQ=21)


                                                       ggCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtt                                      <  1:5350399/101‑1 (MQ=21)


                                                       ggCTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGGCtt                                      >  2:3293246/1‑101 (MQ=21)


                                                         cTGGCGCGTGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.GCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  2:1437643/101‑1 (MQ=21)


                                                         cTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  2:4869272/101‑1 (MQ=21)


                                                         cTGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  2:155874/101‑1 (MQ=21)


                                                          tGGCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAAGTCATTTc                                   >  2:5260801/1‑101 (MQ=18)


                                                           ggCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  1:1745297/101‑1 (MQ=21)


                                                           ggCGCGCGCTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  2:1361374/101‑1 (MQ=21)


                                                            gcgcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCCATTCACCCCCCGGGAAAATTTGGGCCAAGAAAAAAAAGGGTTTTTct                                  >  2:4171903/1‑100 (MQ=9)


                                                            gcgcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                 <  1:2668849/101‑1 (MQ=21)


                                                            gcgcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                 <  1:4587556/101‑1 (MQ=21)


                                                             cgcgcggTCTGAGACG.AAACGG.GGG.GCGCAGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  1:4756132/101‑1 (MQ=21)


                                                             cgcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  1:4215930/101‑1 (MQ=21)


                                                              gcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  2:214335/101‑1 (MQ=21)


                                                              gcgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  2:2039042/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  2:4004732/101‑1 (MQ=21)


                                                               cgcgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  1:993367/101‑1 (MQ=21)


                                                                 cgcTCTGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                            <  1:1003710/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         <  1:3873153/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         <  1:5314409/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         <  1:2501970/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         <  1:2074366/101‑1 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         >  2:2496858/1‑101 (MQ=21)


                                                                    tctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         >  2:1505667/1‑101 (MQ=21)


                                                                    tcggagaCG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATGAAGAAGGTCTTTTGCTTACATCtt                         <  2:173324/98‑1 (MQ=17)


                                                                     ctGAGACG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                        <  1:5036225/101‑1 (MQ=21)


                                                                       gagaCG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTTGGGCAAGATAAAGAAGGGCTTTTTCTTTTTTCTTTcc                      >  2:4983817/1‑101 (MQ=17)


                                                                       gagaCG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGGCTTTTTCTTTCATCTTTcc                      >  2:127608/1‑101 (MQ=18)


                                                                        agaCG.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                     >  1:4487235/1‑101 (MQ=21)


                                                                            g.AAACGG.GGG.GCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                 <  1:1183627/101‑1 (MQ=21)


                                                                              aaacgg.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                <  2:1746015/96‑1 (MQ=21)


                                                                              aaacgg.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                >  1:802128/6‑101 (MQ=21)


                                                                                gcag.ggg.gCGCTG.CCCC.CTC.TT..GGCC.TTTGG.CCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggg        >  1:476340/4‑101 (MQ=0)


                                                                                acgg.ggg.gCGCTGCCCCC.CTT.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTT.GGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             <  1:3639029/98‑1 (MQ=21)


                                                                                 cgg.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             <  2:3847742/99‑1 (MQ=25)


                                                                                 cgg.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             <  1:3815916/99‑1 (MQ=25)


                                                                                 cag.ggg.gCGCTG.CCCC.CTC.TT..GGCC.TTTGG.CCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg       >  1:728053/3‑101 (MQ=0)


                                                                                  ag.ggg.gCGCTGCCCCCTCTT.GG..C..C.TTTGG.CCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg      >  1:1969377/2‑101 (MQ=0)


                                                                                  ag.ggg.gCGCTG.CCCC.CTC.TT..GGCC.TTTGG.CCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg      >  1:3552899/2‑101 (MQ=0)


                                                                                   g.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc           <  1:125581/101‑1 (MQ=25)


                                                                                   g.ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc           <  1:2242135/101‑1 (MQ=25)


                                                                                     ggg.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATAACCCg          >  1:1221029/1‑101 (MQ=25)


                                                                                     ggg.gCGATGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg          <  1:918241/101‑1 (MQ=25)


                                                                                       g.gCGCTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggg        <  1:5368097/101‑1 (MQ=37)


                                                                                           gcTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa     >  2:4478594/1‑101 (MQ=37)


                                                                                           gcTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa     >  1:5471362/1‑101 (MQ=37)


                                                                                           gcTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa     <  2:3345456/101‑1 (MQ=37)


                                                                                           gcTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa     <  2:3056052/101‑1 (MQ=37)


                                                                                            cTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg    >  1:4357674/1‑101 (MQ=37)


                                                                                            cTGCCCCC.CTC.TT..GGCC.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg    >  1:553744/1‑101 (MQ=37)


CTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.G.GGG.GCGCTGCCCCC.CTC.CT..G..C.TTCGG.CCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566456‑2566644

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG