Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	75.2	20	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (5/15):  ref base (0/0):  total (5/15)

GCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCC.CGCCCAGACCGGCGCCGA  >  NC_014034/2602953‑2603143                                                                                                  |                                                                                               gggggggggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCGCGCTGGGGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGAc                                                                                             <  1:3854148/99‑1 (MQ=255)    gggggggCGGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGcc                                                                                          <  1:4171490/98‑1 (MQ=255)    ggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGcc                                                                                          <  2:3338504/101‑1 (MQ=255)     gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                         <  2:1207084/101‑1 (MQ=255)     gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                         <  1:2625638/101‑1 (MQ=255)       gggcCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcgc                                                                                       <  2:3181054/101‑1 (MQ=255)          gCTGCCCCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGTCGGGGGCCGCGTTCGACCGCGCCGCCGTCACCCAGCCCCCCGCCGCGACGCCCGCGGc                                                                                    <  2:2867819/101‑1 (MQ=255)              ccgccgCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccccc                                                                                <  1:1502124/101‑1 (MQ=255)                gccgcGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGc                                                                              <  1:5587443/101‑1 (MQ=255)                  cgcgGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCCCCCCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCa                                                                            <  1:5779433/101‑1 (MQ=255)                      ggCAGCGAGCGGATCGACATCCCCCCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                        <  2:1535251/101‑1 (MQ=255)                         agcgagcgGATCGACATCACCTCGCTGGCGGGGGGCGCGGGCGACCGCGCCGCCCCCACCCACCCGCCCGCCGCCACGCCCGCGGCCCCCCCCACCCCGcc                                                                     >  2:2532352/1‑101 (MQ=255)                          gcgaccgGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGccc                                                                    <  1:2868384/101‑1 (MQ=255)                               cgGATCGACATCACCTCGCTGGCGGGGGGCGCGGTCGACCGCGCCGCCGCCACCCCCCCCCCCCCCGCGGCCCCCCCGGCCCCCCCCCCCCCGcccccgcc                                                               >  2:3558863/1‑97 (MQ=255)                                ggATCGACATCACCTCGCTGGCGGGGGCCGCGCCCCGCCGGGCCCCCCGGCCCCCCACGCCCGCCGCGCCGCCCCCCGGCCCCGCCCCCCCCccccccccc                                                              >  2:1989559/1‑96 (MQ=255)                                ggATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCCCCGGCACCCACCCCACCCCCCCCGCGCCCGCCGCCCCCCCCCCCCCCcccccgccc                                                              >  2:5737616/1‑96 (MQ=255)                                                 cgggCGGGGCCCGGTACCGGCCGCGCCGCCTGCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAg                                             <  1:5737911/99‑1 (MQ=255)                                                            cgcgATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGa                                  >  1:1707814/1‑101 (MQ=255)                                                              cccctccccGCCCCCCCGTCCCCCCCCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGACCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaa                                <  1:1653439/94‑1 (MQ=255)                                                                   gACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGACCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaaagatc                           >  2:1653345/1‑96 (MQ=255)                                                                    cccGCCCCGCCGCCCCCCACCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgcc                          <  1:3559080/100‑1 (MQ=255)                                                                              cgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccc.cgccc               >  2:3645115/1‑101 (MQ=255)                                                                                  aCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCC.CGCCCAGAc           >  2:2442023/1‑101 (MQ=255)                                                                                    cccgcccccCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCcgcccctcgccctcctc          <  2:1707715/98‑6 (MQ=255)                                                                                           cccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCC.CGCCCAGACCGGCgccga  >  1:1766838/1‑101 (MQ=255)                                                                                                  |                                                                                               GCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCC.CGCCCAGACCGGCGCCGA  >  NC_014034/2602953‑2603143 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 39 ≤  ATCG