Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,191 G→C 17.2% intergenic (‑167/+109) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1910G→C17.2% 73.2 0.0 ‑0.0171intergenic (‑167/+109)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/15):  ref base (69/72):  total (84/87)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 180 total aligned reads displayed.

GCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640097‑2640291
                                                                                                 |                                                                                                    
gcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                                                                 <  2:5268482/101‑1 (MQ=37)
gcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                                                                 <  2:2526534/101‑1 (MQ=37)
 cggcAGGCGTGTCAACCGTGGCGCTTTTGCGGAGGCAAAGGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  1:2291817/101‑1 (MQ=25)
 cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  2:4593755/101‑1 (MQ=37)
  ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GTC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:3175682/101‑1 (MQ=37)
  ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  1:1830338/101‑1 (MQ=37)
   gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                              <  1:285942/101‑1 (MQ=37)
   gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                              <  2:1535203/101‑1 (MQ=37)
    cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                             <  1:2467963/101‑1 (MQ=25)
     aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCACCTCTT.G.GCCTTCGGCCAATTCACCCCCCCCGGTATTTGGAGCAAGATGa                                                                                            >  2:1223828/1‑101 (MQ=25)
      ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  2:2036113/101‑1 (MQ=25)
         gtgtCAGCCGGGGCCCTTTTGCGCAGGGAAAAGGGGGGCGCTGCCCCCCTCCT.G.GCCTTCGGGCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                        <  1:4690161/101‑3 (MQ=18)
         gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                        <  1:154107/101‑3 (MQ=25)
         gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                        <  1:397080/101‑3 (MQ=25)
             cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTACCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                   <  2:1437485/101‑1 (MQ=25)
              aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  1:4438297/101‑1 (MQ=21)
                ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                <  2:4538823/101‑1 (MQ=25)
                   tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                             <  2:4230573/101‑1 (MQ=25)
                      cgcTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCTT.G.GCCTTCGGCCAATTTACCCCCCCCGGGATTTTGAGCAAGATG.AAAGACCATGGGTTTAtt                                                                          >  1:881576/1‑100 (MQ=255)
                       gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                         <  1:1050008/101‑1 (MQ=25)
                             gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  2:4110343/101‑1 (MQ=25)
                                cacgCAAAAGGGGGGCGCTGCCCCCCTCTTGG.G.CTTCGGCCCATTTACCCCCCGAGGGATTTGGGGCAAAATG.AAAGACCCTGGGTTTATTTTGCCTCaaa                                                                >  1:3859305/4‑101 (MQ=16)
                                   gCAAAAGGGGCGCGCCTCCCCCCTCTT.G.GCCTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:2183357/101‑1 (MQ=25)
                                    tgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCTGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                           <  1:4925300/99‑1 (MQ=17)
                                      aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  1:4119471/101‑1 (MQ=17)
                                      aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  2:4791669/1‑101 (MQ=17)
                                      aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  2:24664/101‑1 (MQ=17)
                                      aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  1:5639432/101‑1 (MQ=17)
                                       aaagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  1:1855996/4‑101 (MQ=17)
                                       aaGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                         <  2:4554574/101‑1 (MQ=25)
                                        aagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                       >  1:1167496/3‑101 (MQ=18)
                                        aagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                       <  1:2114397/99‑1 (MQ=18)
                                        aagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGACCCAAATATCCCggg                                                       >  1:3353817/3‑101 (MQ=17)
                                         agggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      <  2:5208637/100‑1 (MQ=18)
                                          gggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGTAAGGA..TGGGGTTTATCTTGCCCCAAATATTCCggggg                                                     >  2:5542638/1‑101 (MQ=17)
                                          gggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                      >  2:4815310/1‑101 (MQ=25)
                                           ggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                    <  1:2005554/101‑2 (MQ=18)
                                            gggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                   <  1:3393296/101‑3 (MQ=18)
                                             ggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                  >  2:1178424/1‑98 (MQ=18)
                                              gcgcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:3114982/101‑5 (MQ=21)
                                                gcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgcgggtgaggc                                               >  2:4322320/1‑95 (MQ=21)
                                                gcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                >  1:3601608/1‑101 (MQ=25)
                                                gcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                >  2:2350610/1‑101 (MQ=25)
                                                  tGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcga                                             >  1:1368990/1‑94 (MQ=21)
                                                   gCCCCCCTCTT.G.GCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcgaa                                            <  2:70105/101‑10 (MQ=25)
                                                   gCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGCGGGGAGTCCtc                                             >  2:3333491/1‑100 (MQ=25)
                                                   gCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCCAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCCGGGGGAGGCCTg                                             >  2:609878/1‑101 (MQ=25)
                                                    ccccccTCTT.G.GCCTTTGGCCAATTCACCCCCCGAGGTATTTGGAGCCAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCtta                                            >  1:1424258/1‑99 (MQ=25)
                                                     cccccTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCTGaa                                           >  2:3938958/1‑101 (MQ=25)
                                                      ccccTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  2:597142/1‑101 (MQ=37)
                                                       cccTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  2:4566733/1‑101 (MQ=37)
                                                         ctctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       <  1:5235355/101‑1 (MQ=37)
                                                         ctctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  2:4410668/1‑101 (MQ=37)
                                                          tctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  2:5167840/101‑1 (MQ=37)
                                                           ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                     <  2:2122091/101‑1 (MQ=37)
                                                              ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    <  1:1039159/96‑1 (MQ=37)
                                                               tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:1488014/5‑101 (MQ=37)
                                                               tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:1281741/5‑101 (MQ=37)
                                                                 gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 >  2:1477467/3‑101 (MQ=37)
                                                                     tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             >  2:112307/1‑101 (MQ=255)
                                                                      cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGTGAGTCCTGAAAGGACGGGGGGCaga                            >  1:3523901/1‑101 (MQ=37)
                                                                      cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  1:83945/1‑101 (MQ=255)
                                                                       ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  2:1950199/1‑101 (MQ=37)
                                                                         ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                         >  1:3694064/1‑101 (MQ=37)
                                                                          cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  1:617288/1‑101 (MQ=255)
                                                                          cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  1:3734828/1‑101 (MQ=37)
                                                                           aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  1:4386708/1‑101 (MQ=37)
                                                                            aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAATGACGGGGGGCAGAGccccc                      >  1:3248765/1‑101 (MQ=37)
                                                                            aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  2:2729966/1‑101 (MQ=255)
                                                                            aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  2:3229352/1‑101 (MQ=37)
                                                                              tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  1:2473088/4‑101 (MQ=255)
                                                                               caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:3078918/3‑101 (MQ=255)
                                                                                 ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  1:176/101‑1 (MQ=255)
                                                                                  cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  2:952536/101‑1 (MQ=255)
                                                                                  cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  1:2926825/101‑1 (MQ=255)
                                                                                  cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  1:4054548/101‑1 (MQ=255)
                                                                                   ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               >  2:3234337/1‑101 (MQ=255)
                                                                                     ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCCCCCgggc             >  1:2370244/1‑100 (MQ=25)
                                                                                      cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            >  2:1638340/1‑101 (MQ=255)
                                                                                      cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            >  1:1931677/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:1697365/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  1:3826016/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           <  2:5661055/101‑1 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:160650/1‑101 (MQ=255)
                                                                                         ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         <  2:3857431/101‑1 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        >  2:4066177/1‑101 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        >  1:1453986/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:2113747/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:857877/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGAGGAGTCCTGAAAGGACGGGGGGCAGAGCACCCCTCCCGGGTCTTtct       >  2:4993926/1‑101 (MQ=37)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:3419261/1‑101 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  1:4713131/101‑1 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  1:57083/101‑1 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  1:4170348/101‑1 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  2:3717429/101‑1 (MQ=255)
                                                                                              ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    <  1:4567009/101‑1 (MQ=255)
                                                                                              ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:4063216/1‑101 (MQ=255)
                                                                                               tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   >  1:5784336/1‑101 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:2249088/1‑101 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:4494020/1‑101 (MQ=255)
                                                                                                 |                                                                                                    
GCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640097‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG