Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	120.3	42	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (14/28):  ref base (0/0):  total (14/28)

AATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747759‑2747950                                                                                                   |                                                                                                  aaaaGGAACCGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgag                                                                                               <  2:4568645/98‑1 (MQ=37)  agaGGAAACGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgaga                                                                                              <  2:1315581/99‑1 (MQ=37)   tAGGAAGCGCGACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                             <  2:3790744/101‑1 (MQ=37)    aGGAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAg                                                                                            <  1:2605790/101‑1 (MQ=25)     ggAACCGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgc                                                                                           <  1:4153523/101‑1 (MQ=25)       aaaCGCGCCCCGCGTTC.ACCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgcgc                                                                                         <  2:5193131/101‑1 (MQ=25)        accgccccccGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcc                                                                                        <  1:5604077/94‑1 (MQ=25)         ccgcgCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                       <  1:675307/100‑1 (MQ=25)         acactaCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                       <  2:193254/96‑1 (MQ=25)         aCGCGCCCGGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                       <  1:4221652/101‑1 (MQ=21)         aCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                       <  1:3168303/101‑1 (MQ=25)          cgcgCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                                      <  2:1577389/101‑1 (MQ=25)            cgcccccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCACCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                    <  1:5208470/93‑1 (MQ=21)            cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                    <  2:1158162/98‑1 (MQ=25)            cgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                    <  1:1874333/98‑1 (MQ=25)              ccccGCTTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  2:5618102/100‑1 (MQ=25)              ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  1:5026240/100‑1 (MQ=25)              ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  2:1599325/100‑1 (MQ=25)              ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  1:726977/100‑1 (MQ=25)              ccccCCGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCccgcc.g                                                                                  <  2:4207865/100‑1 (MQ=21)               cccGTGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  1:3869788/101‑1 (MQ=21)                cccggTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                               <  2:928387/97‑3 (MQ=21)                ccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                               <  1:3724269/101‑3 (MQ=25)                 cgcgTCC.CCCCGCCCGCAGCGCCCGGTGGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  2:4176450/101‑4 (MQ=21)                   cgTTG.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                            <  1:2815315/101‑6 (MQ=25)                    ctcccccccGCCCGCAGCGCCGGGTGGCGGCATGCCCCCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                            <  1:1139008/96‑6 (MQ=18)                     tgt.CCCCGCCCGCGGCGCCCGGTTGCGGCATGGCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                          <  2:5117143/99‑8 (MQ=21)                                            ttGCGGCAGGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGC.CCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGcc                                                     <  2:1423690/101‑1 (MQ=17)                                                   aTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCC.GCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGGCTTTGGGCCCTCcggg                                              >  2:617932/1‑101 (MQ=18)                                                                                  gcgcCCGG.CCCGGCGGGCGAGAGGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAgg               <  1:2443025/101‑1 (MQ=18)                                                                                    gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg             >  1:5324893/1‑101 (MQ=18)                                                                                    gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg             >  1:453111/1‑101 (MQ=18)                                                                                       cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg          >  2:73971/1‑101 (MQ=18)                                                                                       cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg          >  2:4163181/1‑101 (MQ=18)                                                                                       cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg          >  2:614539/1‑101 (MQ=18)                                                                                        gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  2:3932973/1‑101 (MQ=18)                                                                                        gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCCTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTgg         >  1:1287893/1‑101 (MQ=18)                                                                                          gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc        >  2:4724490/6‑101 (MQ=18)                                                                                            ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt      >  2:4937026/4‑101 (MQ=21)                                                                                             cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt     >  2:5063034/3‑101 (MQ=21)                                                                                              ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg    >  1:4392729/2‑101 (MQ=21)                                                                                               gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg   >  1:5080120/1‑101 (MQ=21)                                                                                                cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc  >  2:396339/1‑101 (MQ=21)                                                                                                cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc  >  1:4178537/1‑101 (MQ=21)                                                                                                   |                                                                                                  AATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747759‑2747950 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG