Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,891,981 T→G 100% S460A (TCG→GCG)  thiP → thiamine ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,891,9810T→G100.0% 907.6 243S460A (TCG→GCG) thiPthiamine ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (132/111):  ref base (0/0):  total (132/111)

Only 100 of 256 total aligned reads displayed.

CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891884‑2892078
                                                                                                 |                                                                                                 
ccGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGc                                                                                                >  1:2986227/1‑101 (MQ=255)
 cGGCTGCGGTTCCTCACCCTGCCCCGGCTGGGCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCt                                                                                               >  1:5171380/1‑101 (MQ=255)
 cGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCt                                                                                               <  2:3248799/101‑1 (MQ=255)
 cGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCt                                                                                               >  1:2121622/1‑101 (MQ=255)
  ggCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTg                                                                                              >  2:1658032/1‑101 (MQ=255)
    cTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                            >  1:114611/1‑101 (MQ=255)
    cTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                            >  1:4060976/1‑101 (MQ=255)
       cGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTc                                                                                         >  2:3065729/1‑101 (MQ=255)
        ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        >  1:140116/1‑101 (MQ=255)
         gTTCCTCCCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  2:3584235/101‑1 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGCCGGGCTTTGCCGCGGGGATTGCCGCGCCCTTTTCGCTGGGCGATCTGGGCGCCATCGCGCTGTTTTcca                                                                                       >  1:1866963/1‑100 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  1:5642533/101‑1 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  1:1625672/101‑1 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  2:2986060/101‑1 (MQ=255)
          ttCCTCGCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGa                                                                                      <  1:2770475/101‑1 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGGCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  1:3280582/1‑101 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  1:2235917/1‑101 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  2:3769393/1‑101 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  2:285530/1‑101 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  1:4472277/1‑101 (MQ=255)
            ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                    <  1:1311340/101‑1 (MQ=255)
             cccccccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATgg                                                                                   <  1:4844640/97‑1 (MQ=255)
               cACCCTGCCCTGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGcc                                                                                 <  2:5665040/101‑1 (MQ=255)
               cACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGcc                                                                                 >  1:1497566/1‑101 (MQ=255)
               cACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGcc                                                                                 <  2:5746245/101‑1 (MQ=255)
                aCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                <  2:2547433/101‑1 (MQ=255)
                  ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                              <  1:2471374/101‑1 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                             <  2:114591/101‑1 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                             <  1:2597803/101‑1 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                             <  2:1856815/101‑1 (MQ=255)
                    tGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGc                                                                            <  2:4264900/101‑1 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           >  1:2063361/1‑101 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           <  2:2317144/101‑1 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           <  2:3885060/101‑1 (MQ=255)
                      ccccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCa                                                                          >  2:1752026/1‑101 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGGGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         >  1:570591/1‑101 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         <  1:1414705/101‑1 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCCTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         >  2:4214089/1‑101 (MQ=255)
                        ccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCTCAGCCAGa                                                                        <  2:5161257/101‑1 (MQ=255)
                        ccGGCTGGCCCGGCCGACGGGCCTTGCCGCGGGTATTTCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGa                                                                        <  2:5460651/101‑1 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      >  1:3480353/1‑101 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      >  1:2030798/1‑101 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      <  1:2345926/101‑1 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGGTCTGGGGGGCATCGCGCTGTTTTCCTTTGGCCGCAGCCAGAccccc                                                                   >  2:3493374/1‑99 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   <  1:1187122/101‑1 (MQ=255)
                              ggCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGc                                                                  <  1:4412250/101‑1 (MQ=255)
                                cccGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCg                                                                <  2:5374234/101‑1 (MQ=255)
                                cccGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCg                                                                <  1:3455760/101‑1 (MQ=255)
                                   ggCCGACGGGCTATGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCtt                                                             >  1:3123013/1‑101 (MQ=255)
                                    gCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTg                                                            >  2:1463837/1‑101 (MQ=255)
                                     ccGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGc                                                           >  2:1038848/1‑101 (MQ=255)
                                      cGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGcc                                                          >  1:3612452/1‑101 (MQ=255)
                                         cGGGCATGGCCGCGGGGATTGCAGCGGGCTTTTCTATAGGAGATCTGAGAGCCATGGCGCCATTTTCCGATGGGCTCCACCAGACCCTGCCGCTTGTCGTc                                                       >  2:653217/1‑101 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      <  2:808809/101‑1 (MQ=255)
                                            gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    >  1:1715482/1‑101 (MQ=255)
                                            gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCACCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    >  2:204810/1‑101 (MQ=255)
                                                 gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  2:5052029/1‑101 (MQ=255)
                                                 gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  2:93979/1‑101 (MQ=255)
                                                     cgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATg                                           >  2:1782491/1‑101 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                          >  2:3122037/1‑101 (MQ=255)
                                                        ggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGc                                        >  1:32946/1‑101 (MQ=255)
                                                        ggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGc                                        <  2:5262577/101‑1 (MQ=255)
                                                         gATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCa                                       <  2:3719238/101‑1 (MQ=255)
                                                         gATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCa                                       <  2:5457782/101‑1 (MQ=255)
                                                          aTTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAg                                      <  1:3712680/101‑1 (MQ=255)
                                                           ttGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGc                                     >  1:3376298/1‑101 (MQ=255)
                                                            tGCCGCGGCCTTTTCGATGGTCGATCTGGGCGCCATCGCGCTGTTTTCCGATGCCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCt                                    <  2:3332865/101‑1 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   <  2:5171094/101‑1 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   >  1:390949/1‑101 (MQ=255)
                                                               gatctccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 <  2:150904/96‑1 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 >  1:448827/1‑101 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 <  1:4707325/101‑1 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 <  2:2301/101‑1 (MQ=255)
                                                                gcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCg                                <  1:4383223/101‑1 (MQ=255)
                                                                 cgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGc                               >  1:780344/1‑101 (MQ=255)
                                                                    ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:4586041/101‑1 (MQ=255)
                                                                     cTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATgg                           >  2:2231575/1‑101 (MQ=255)
                                                                     cTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATgg                           >  1:851404/1‑101 (MQ=255)
                                                                        ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                        <  1:629653/101‑1 (MQ=255)
                                                                        ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                        >  1:2801237/1‑101 (MQ=255)
                                                                           gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                     <  1:1463930/101‑1 (MQ=255)
                                                                            aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                    >  2:3691419/1‑101 (MQ=255)
                                                                             tGGGCGATCTGGGCGCCCTCGCGCTGTTTTCCGATGGCCGCAGCCAGCCCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   <  2:1979250/101‑1 (MQ=255)
                                                                             tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   <  2:4518980/101‑1 (MQ=255)
                                                                              gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                  <  2:3288573/101‑1 (MQ=255)
                                                                              gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                  >  2:3022/1‑101 (MQ=255)
                                                                                gCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcgcg                <  1:4194934/101‑1 (MQ=255)
                                                                                  gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg              <  2:3173185/101‑1 (MQ=255)
                                                                                  gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg              >  1:735391/1‑101 (MQ=255)
                                                                                   aTCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgc             >  2:3986815/1‑101 (MQ=255)
                                                                                     cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc           >  2:4812681/1‑101 (MQ=255)
                                                                                       gggCCCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg         >  2:3383260/1‑101 (MQ=255)
                                                                                        ggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGc        >  2:2444702/1‑101 (MQ=255)
                                                                                           gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCttc     >  1:5426685/1‑100 (MQ=255)
                                                                                            ccATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGc    >  2:4151700/1‑101 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTggc   >  2:1303608/1‑99 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc   >  1:5524730/1‑101 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc   >  2:261672/1‑101 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc   >  1:1093055/1‑101 (MQ=255)
                                                                                              aTCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCt  >  2:4859626/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891884‑2892078

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG