Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,712 C→G 4.5% intergenic (+55/‑105) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7120C→G 4.5% 21.1 ‑1.7 ‑1.3294intergenic (+55/‑105)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (10/4):  ref base (144/135):  total (155/139)
Fisher's exact test strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.31e-02

Only 100 of 318 total aligned reads displayed.

GCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGA  >  NC_014034/2950612‑2950812
                                                                                                    |                                                                                                      
   cGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACg                                                                                                     <  2:3678440/101‑1 (MQ=255)
     gggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGcc                                                                                                   >  2:1234531/1‑101 (MQ=255)
         gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                               >  2:720293/1‑101 (MQ=255)
         gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                               <  1:2909340/101‑1 (MQ=255)
            gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCTAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                            <  1:4955264/101‑1 (MQ=255)
             cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCAt                                                                                           >  1:4791347/1‑101 (MQ=255)
                tCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  2:687318/101‑1 (MQ=255)
                tCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  2:5529957/101‑1 (MQ=255)
                tCGCCGCAATAGGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  1:4056812/101‑1 (MQ=255)
                 cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTg                                                                                       >  1:5570170/1‑101 (MQ=255)
                 cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTg                                                                                       <  1:1157786/101‑1 (MQ=255)
                 cgccgcAATATGCGGAACTTGATTTCGGAAGAAGGCCGGGGGCTCTGCCCCGGGGCCCCCGGGATATTTGAGGCAAGATGAAACGCGCCCCTTTCATCTTg                                                                                       <  2:4106771/101‑1 (MQ=25)
                   ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcc                                                                                     >  1:5421144/1‑101 (MQ=255)
                    cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                    >  2:2837879/1‑101 (MQ=255)
                      cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                  >  1:3260834/1‑101 (MQ=255)
                      cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                  >  2:2691287/1‑101 (MQ=255)
                       aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                 >  1:3545621/1‑101 (MQ=255)
                       aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                 >  2:3398517/1‑101 (MQ=255)
                        atatGCGGAACTTGATTTCTGGAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                >  1:4264471/1‑101 (MQ=255)
                        atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                <  2:1685973/101‑1 (MQ=255)
                        atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                >  1:382389/1‑101 (MQ=255)
                         tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACTCCCCTTTCATCTTGCCCCAAat                                                                               >  1:882584/1‑101 (MQ=255)
                         tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAat                                                                               >  2:1873947/1‑101 (MQ=255)
                          atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatt                                                                              >  1:1435725/1‑100 (MQ=255)
                          atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                              <  1:5208323/101‑1 (MQ=255)
                          atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                              >  1:637554/1‑101 (MQ=255)
                           tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                             <  1:3987881/101‑1 (MQ=255)
                           tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                             >  1:3205776/1‑101 (MQ=255)
                             cgtaaCTTGATTACTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                           <  2:5146320/98‑1 (MQ=255)
                             cGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                           <  2:4178032/101‑1 (MQ=255)
                               gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                        <  2:2962597/101‑1 (MQ=255)
                                  cTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gggg                                                                     <  2:4529539/101‑1 (MQ=255)
                                   ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                    >  2:5674340/1‑101 (MQ=255)
                                     gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGGGGCAAGATGAAACACGCCCCTTTCGTCTTGCCCCAAAAATCCC.GGGGGTg                                                                  >  1:993147/1‑101 (MQ=255)
                                     gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                  >  2:2912788/1‑101 (MQ=255)
                                      aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                                 >  1:5596187/1‑101 (MQ=255)
                                      aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                                 <  1:2197245/101‑1 (MQ=255)
                                        ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                               <  2:5569878/101‑1 (MQ=255)
                                        ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                               >  1:4587777/1‑101 (MQ=255)
                                        ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                               <  1:58355/101‑1 (MQ=255)
                                         tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGCATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                              <  2:1354776/101‑1 (MQ=255)
                                          cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                             <  2:5223025/101‑1 (MQ=255)
                                              agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                         <  1:4447134/101‑1 (MQ=255)
                                               gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGt                                                        <  2:4273973/101‑1 (MQ=255)
                                                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                      >  1:1488375/1‑101 (MQ=255)
                                                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                      <  2:651759/101‑1 (MQ=255)
                                                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                      >  1:1575878/1‑101 (MQ=255)
                                                 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGCCa                                                      >  1:5353704/1‑101 (MQ=255)
                                                  agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                     <  2:1598345/101‑1 (MQ=255)
                                                      gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                 >  2:2298068/1‑101 (MQ=255)
                                                      gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                 >  2:4163157/1‑101 (MQ=255)
                                                       ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaaa                                                <  1:3886646/101‑1 (MQ=255)
                                                         ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                              <  1:3740039/101‑1 (MQ=255)
                                                         ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                              >  1:416997/1‑101 (MQ=255)
                                                         ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAaga                                              >  2:1097871/1‑101 (MQ=255)
                                                           ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.gg                                           <  2:2063494/101‑1 (MQ=255)
                                                             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGA.ggag                                         >  1:4536801/1‑99 (MQ=255)
                                                              cttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGA.gggggg                                       >  2:39597/10‑100 (MQ=25)
                                                               gcttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGA.GGGGGCa                                      >  1:5070536/10‑101 (MQ=2)
                                                               gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGc                                       <  2:3205587/101‑1 (MQ=255)
                                                                cttcgccccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATACCCC.AGGGGTGAATTGGCC.AAAGGCCAAGA.GGGGGCAg                                     <  1:1967811/93‑1 (MQ=2)
                                                                cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCa                                      <  2:3055159/101‑1 (MQ=255)
                                                                  tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGA.GGGGGCAGcg                                   >  1:4704118/7‑100 (MQ=2)
                                                                  tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGCGAATTGGCC.AAAGGCCAAGA.GGGGGCAGcg                                   >  1:3392466/7‑100 (MQ=2)
                                                                   ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCt                                   <  2:3768320/101‑1 (MQ=255)
                                                                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTc                                  >  1:1827839/1‑101 (MQ=255)
                                                                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTc                                  >  2:4561523/1‑101 (MQ=255)
                                                                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGA.GGGGGCAGCGccc                                >  2:4719377/4‑101 (MQ=2)
                                                                      gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTccc                                <  1:775123/101‑1 (MQ=255)
                                                                       aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTcccc                               <  1:1874057/101‑1 (MQ=255)
                                                                       aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTcccc                               >  2:2224105/1‑101 (MQ=255)
                                                                        cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTccccc                              <  1:2517496/101‑1 (MQ=255)
                                                                            cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGcc                          <  1:1994509/101‑1 (MQ=255)
                                                                             gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGccc                         >  2:1468973/1‑101 (MQ=255)
                                                                              ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAGGGGGGCAGCGCCCCCttttcg                        >  2:5377029/1‑96 (MQ=17)
                                                                              ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAGGGGGGCAGCGCCCCCttttcg                        >  1:1741932/1‑96 (MQ=17)
                                                                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGcctc                        <  1:2805747/101‑3 (MQ=255)
                                                                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGcccc                        >  2:4846190/1‑101 (MQ=255)
                                                                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGcccc                        >  1:5462442/1‑101 (MQ=255)
                                                                               gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCg                       <  2:382345/101‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGc                     >  1:4108835/1‑101 (MQ=255)
                                                                                  atTTGAGGCAAGATGAAACACGCCCCTTTCAACTGGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAGGA.GTGGGCAGCTTCCCCTGCCCCGGCt                    >  2:2403858/1‑101 (MQ=37)
                                                                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgtg                 >  2:3310272/1‑99 (MQ=255)
                                                                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgag                 >  1:2431149/1‑101 (MQ=255)
                                                                                          cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTgagggagg            >  1:2732076/1‑101 (MQ=255)
                                                                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc           >  1:2321227/1‑101 (MQ=255)
                                                                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc           <  1:3707497/101‑1 (MQ=255)
                                                                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc           >  2:504913/1‑101 (MQ=255)
                                                                                            aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg          <  1:2691436/101‑1 (MQ=255)
                                                                                            aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg          >  1:5304664/1‑101 (MQ=255)
                                                                                              aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAGATGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa        >  2:5002182/1‑101 (MQ=255)
                                                                                               tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa       <  1:694492/101‑1 (MQ=255)
                                                                                               tGAAACACGCCCCTTTCATCGTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa       <  2:2556331/101‑1 (MQ=255)
                                                                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc      >  2:1118253/1‑101 (MQ=255)
                                                                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc      >  1:1188562/1‑101 (MQ=255)
                                                                                                 aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt     >  2:3613295/1‑101 (MQ=255)
                                                                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg   <  2:1554978/101‑1 (MQ=255)
                                                                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg   >  1:3002841/1‑101 (MQ=255)
                                                                                                    cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa  >  1:1258849/1‑101 (MQ=255)
                                                                                                    cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa  >  2:626773/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
GCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGA.GGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGA  >  NC_014034/2950612‑2950812

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG