Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,973,819 T→A 100% V311D (GTT→GAT)  RCAP_rcc02786 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,973,8190T→A100.0% 969.9 252V311D (GTT→GAT) RCAP_rcc02786hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (127/124):  ref base (0/1):  total (127/125)

Only 100 of 263 total aligned reads displayed.

CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916
                                                                                                  |                                                                                                 
ccTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATc                                                                                                 <  1:5564499/101‑1 (MQ=255)
ccTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATc                                                                                                 >  2:3757755/1‑101 (MQ=255)
 cTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCt                                                                                                >  1:1055057/1‑101 (MQ=255)
  tGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTg                                                                                               <  1:4213445/101‑1 (MQ=255)
   gcgcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTgg                                                                                              <  2:1033417/101‑1 (MQ=255)
   gcgcAATGGCGGCGGCCCCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTgg                                                                                              <  2:707745/101‑1 (MQ=255)
    cgcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTggg                                                                                             <  1:2675083/101‑1 (MQ=255)
    cgcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTggg                                                                                             >  1:4394886/1‑101 (MQ=255)
     gcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgc                                                                                            <  2:1520004/101‑1 (MQ=255)
      cAATGTCGGCGGCACCATCGACGGGGCGGCGCAGATGGGCGACGGGGCCGATCTGCTGATCAATACGGGCGGTCGGATCCTTGGTGATGTCGTTCTgggtg                                                                                           >  2:3782027/1‑99 (MQ=255)
      cAATGGCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgcg                                                                                           <  2:4860896/101‑1 (MQ=255)
          gtggggggcACCATCGAGGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGGGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggg                                                                                       <  2:3406272/95‑1 (MQ=255)
          gTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggg                                                                                       >  2:1281276/1‑101 (MQ=255)
            cggcggCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggggg                                                                                     <  1:887435/101‑1 (MQ=255)
               cggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCg                                                                                  >  1:4567499/1‑101 (MQ=255)
                    ccATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGc                                                                             <  2:2489788/101‑1 (MQ=255)
                    ccANCGNCGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGc                                                                             <  1:691435/101‑1 (MQ=255)
                     cATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCt                                                                            <  1:4062557/101‑1 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           >  2:1546486/1‑101 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           >  2:3472088/1‑101 (MQ=255)
                       tCGAGGGGGCGGTGCGGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTac                                                                          <  2:3594195/101‑1 (MQ=255)
                       tCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTac                                                                          <  2:160615/101‑1 (MQ=255)
                       tCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTac                                                                          <  1:5714389/101‑1 (MQ=255)
                        cGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacg                                                                         <  1:2980429/101‑1 (MQ=255)
                         gACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCCGTacga                                                                        >  2:2156176/1‑101 (MQ=255)
                          aCGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacgac                                                                       <  1:186191/101‑1 (MQ=255)
                          aCGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacgac                                                                       >  1:3778890/1‑101 (MQ=255)
                             gggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACggg                                                                    >  1:3067308/1‑101 (MQ=255)
                              ggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGgc                                                                   <  2:2097552/101‑1 (MQ=255)
                                cGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAAGTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGgcgc                                                                 <  2:500796/101‑1 (MQ=255)
                                 ggTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCa                                                                <  2:5393175/101‑1 (MQ=255)
                                  gTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAg                                                               >  2:4553482/1‑101 (MQ=255)
                                    gCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcg                                                             >  2:4310002/1‑101 (MQ=255)
                                     cataTGGGCGACGGGGCCTATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcgg                                                            <  2:85212/98‑1 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           >  2:2971705/1‑101 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           <  1:2789500/101‑1 (MQ=255)
                                       gATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggcg                                                          <  1:1575130/101‑1 (MQ=255)
                                       gATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggcg                                                          >  2:5639329/1‑101 (MQ=255)
                                        aTGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgc                                                         >  1:2083420/1‑101 (MQ=255)
                                          gggCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGACCCTTGGCGATGTCGATCTCGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgcgc                                                       >  1:4782691/1‑101 (MQ=255)
                                           ggCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGGGCAGCGGCGCGCt                                                      >  2:2629735/1‑101 (MQ=255)
                                           ggCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCt                                                      <  2:3349484/101‑1 (MQ=255)
                                           ggCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCt                                                      <  2:3070880/101‑1 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    >  2:2572689/1‑101 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    <  2:322029/101‑1 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    <  1:4470590/101‑1 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    <  1:3519962/101‑1 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    >  1:2930410/1‑101 (MQ=255)
                                              gACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGa                                                   >  2:354030/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  >  2:3838441/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  >  1:1254601/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  <  1:1694267/101‑1 (MQ=255)
                                                cGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATg                                                 <  2:312464/101‑1 (MQ=255)
                                                cGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATg                                                 >  2:179066/1‑101 (MQ=255)
                                                  gggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGc                                               >  1:833235/1‑101 (MQ=255)
                                                    gCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGCTGGCAg                                             >  2:5193454/1‑101 (MQ=255)
                                                    gCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAg                                             <  1:1188335/101‑1 (MQ=255)
                                                     ccGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGc                                            >  1:34543/1‑101 (MQ=255)
                                                       gATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGt                                          <  2:1912645/101‑1 (MQ=255)
                                                       gATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGt                                          >  1:3973944/1‑101 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         >  1:2341587/1‑101 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         >  1:1780902/1‑101 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         >  2:5706974/1‑101 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         <  2:4767598/101‑1 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         >  1:5484432/1‑101 (MQ=255)
                                                         tcggctgATCAAGTCGGTTGGGCGGATCCCTGGCGATGATTAACTAGGCGCGGCGGCCGATGGCTACGACAGGCGCAGCGGCGCGCTGGATGGcggatgcc                                        <  2:950418/98‑8 (MQ=255)
                                                         tCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCg                                        <  2:2420984/101‑1 (MQ=255)
                                                          ctgctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGa                                       <  1:101446/101‑1 (MQ=255)
                                                            gctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATg                                     >  1:1779560/1‑101 (MQ=255)
                                                              tgATCAATTGGGGGGGGCGGGTCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   <  1:4816771/101‑1 (MQ=255)
                                                              tgATCAATTCGGGCGGGCGGATCCTTGGCGACGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   >  1:5168535/1‑101 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGCGCCGATCGCTACGACGGGAGCAGCGGCGCGAAGGGTGGCAGCGTAGATggcg                                  >  2:4616702/1‑101 (MQ=255)
                                                                aTCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGGCGATggcgg                                 >  1:1443448/1‑101 (MQ=255)
                                                                aTCAATTCGGGCGGGCGGATCCTTGGCGATGTAGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcgg                                 >  2:3906781/1‑101 (MQ=255)
                                                                  cAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGGCGATGgcggcg                               >  2:5464586/1‑101 (MQ=255)
                                                                   aaTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGgcgc                              >  2:4397505/1‑101 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGGGTCGATGGCGgcgcg                             >  1:1644532/1‑100 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             >  2:1737978/1‑101 (MQ=255)
                                                                      tCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCTGCGGCGGGCTGGATGGCAGCGTGGATGGGGGCGGtgg                           >  2:2862960/1‑100 (MQ=255)
                                                                      tCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGGGTCGATGGCGGCGCgga                           >  2:2010514/1‑101 (MQ=255)
                                                                        gggcgggcGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgatg                         >  1:30270/1‑101 (MQ=255)
                                                                          gcgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgac                       <  2:1178765/101‑1 (MQ=255)
                                                                           cgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacg                      >  2:3515711/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  1:2451038/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  2:5589799/1‑101 (MQ=255)
                                                                             ggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacgac                    <  1:1986619/101‑1 (MQ=255)
                                                                               cggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGacac                  >  1:770558/1‑101 (MQ=255)
                                                                                  aTCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCtt               >  1:3652082/1‑101 (MQ=255)
                                                                                    ccTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCa             <  1:5537262/101‑1 (MQ=255)
                                                                                     cTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCAt            <  1:3815718/101‑1 (MQ=255)
                                                                                     cTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCAt            >  1:3171915/1‑101 (MQ=255)
                                                                                        ggCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCgg         <  2:4075811/101‑1 (MQ=255)
                                                                                          cGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCa       >  1:2499682/1‑101 (MQ=255)
                                                                                           gATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGCCGCCACCTTCATCGGCaa      >  2:596126/1‑101 (MQ=255)
                                                                                             tGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATa    >  2:3722471/1‑101 (MQ=255)
                                                                                              gTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAc   >  2:1368886/1‑101 (MQ=255)
                                                                                              gTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAc   <  2:5168249/101‑1 (MQ=255)
                                                                                              gTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAc   <  2:4827741/101‑1 (MQ=255)
                                                                                              gTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAc   >  1:4728066/1‑101 (MQ=255)
                                                                                               tCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAcc  <  1:2572831/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG