Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,086 G→A 11.0% intergenic (+132/+44) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0860G→A11.0% 75.4 ‑3.2 ‑1.0263intergenic (+132/+44)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (13/16):  ref base (154/80):  total (167/96)
Fisher's exact test strand distribution p-value = 3.94e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-02

Only 100 of 304 total aligned reads displayed.

GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186
                                                                                                     |                                                                                                    
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   >  2:5596744/1‑101 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   <  1:874968/101‑1 (MQ=255)
     aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 <  2:2189145/101‑1 (MQ=255)
       ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                                                               <  2:5086214/101‑1 (MQ=255)
         ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                                                             <  2:2860038/101‑1 (MQ=255)
         ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                                                             >  1:1770861/1‑101 (MQ=255)
         ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                                                             >  2:5160801/1‑101 (MQ=255)
           aaTTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           <  2:1964224/101‑1 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           <  2:3354046/101‑1 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           >  2:5388486/1‑101 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           >  1:4137067/1‑101 (MQ=255)
            aTTCACCCCCCGGAATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                          >  1:5295721/1‑101 (MQ=255)
             ttCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                         <  2:4913549/101‑1 (MQ=255)
             ttCCCCCCCCGGGATATTTGGACCAAGGTGAAAGAAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                         <  1:4964001/101‑1 (MQ=255)
             ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                         >  1:3242320/1‑101 (MQ=255)
             ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                                                         >  2:452708/1‑101 (MQ=255)
               ccccccccGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                                                       <  2:2115181/99‑2 (MQ=18)
               cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggcgcgaaaa                                                                                       >  2:1145990/1‑93 (MQ=255)
                cccccccGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                                                                      >  1:158468/2‑99 (MQ=18)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                      >  2:1602628/1‑101 (MQ=255)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                      >  2:3852015/1‑101 (MQ=255)
                    cccGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                                                                  <  1:2932392/101‑1 (MQ=17)
                     ccGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                                                 <  2:3123127/101‑1 (MQ=255)
                      cGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGTTTTGCCTTTCACCTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                                                                <  1:1034182/101‑1 (MQ=255)
                       gggATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                               >  2:1051926/1‑101 (MQ=255)
                       gggATATGTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc                                                                               <  2:1924305/101‑2 (MQ=17)
                        ggATATTTGGCCCGAGGTCAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                                              <  2:3649428/101‑3 (MQ=17)
                        ggATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                                                              >  1:229248/1‑101 (MQ=255)
                          atatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                                                            >  2:3196483/1‑97 (MQ=18)
                          atatTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                                                            <  2:4486033/101‑1 (MQ=255)
                          atatTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGCGGGGCAGAgcccccctgcc                                                                            >  1:3414072/1‑101 (MQ=255)
                           tatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                           >  2:3289757/1‑96 (MQ=18)
                           tatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                           <  1:4207443/101‑6 (MQ=17)
                            atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                                          >  1:4982735/1‑95 (MQ=18)
                            atTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                          >  1:1227456/1‑101 (MQ=255)
                                ggACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                                                      >  1:4419420/1‑101 (MQ=255)
                                ggACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                                                      <  1:1476666/101‑1 (MQ=255)
                                ggACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                                                      <  1:2347303/101‑1 (MQ=255)
                                 gACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                                                     >  2:2447216/1‑101 (MQ=255)
                                 gACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                                                     <  2:1327249/101‑1 (MQ=255)
                                  aCCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                    <  1:773059/101‑1 (MQ=255)
                                    cAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                  >  1:1631918/1‑101 (MQ=255)
                                         tGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                             >  2:4061082/1‑101 (MQ=255)
                                          gAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                                                            >  1:3700150/1‑101 (MQ=255)
                                            aaGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                          >  1:4507491/1‑101 (MQ=255)
                                            aaGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                          >  1:4719323/1‑101 (MQ=255)
                                            aaGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                          >  1:3165173/1‑101 (MQ=255)
                                             aGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgccc                                                         >  2:3473387/1‑99 (MQ=255)
                                              gCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                        >  1:3549562/1‑101 (MQ=255)
                                              gCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                        >  2:4713592/1‑101 (MQ=255)
                                                aGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCNCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                                                      >  1:1992547/1‑101 (MQ=255)
                                                 gAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                     >  1:2811001/1‑101 (MQ=255)
                                                   t.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                                                   >  1:4590357/1‑101 (MQ=255)
                                                      ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt                                                 <  2:4680695/101‑1 (MQ=255)
                                                       cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                                >  2:4351468/1‑101 (MQ=255)
                                                         gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                              <  1:1161349/101‑1 (MQ=255)
                                                          tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                             <  2:555721/101‑1 (MQ=255)
                                                          tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                             >  2:2724568/1‑101 (MQ=255)
                                                           ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgcc                                            >  2:693364/1‑99 (MQ=255)
                                                           ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                            >  2:5046377/1‑101 (MQ=255)
                                                             gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                          <  2:246247/101‑1 (MQ=255)
                                                               cTTTCACCTTGGCGCAAATATCCCGGGGGGACTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGAGCTCAGGCGTGTTTGGCGAGc                                        >  1:5202033/1‑101 (MQ=255)
                                                                tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       >  2:4327715/1‑101 (MQ=255)
                                                                tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       >  1:509733/1‑101 (MQ=255)
                                                                tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       >  1:669337/1‑101 (MQ=255)
                                                                  tCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGc                                     >  1:554134/1‑101 (MQ=255)
                                                                  tCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGc                                     >  1:5417403/1‑101 (MQ=255)
                                                                   cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct                                    >  1:2541988/1‑101 (MQ=255)
                                                                     ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                  <  2:3731767/101‑1 (MQ=255)
                                                                      ctgggCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                 <  2:5136708/98‑1 (MQ=255)
                                                                      ctgggCGAAAATGCCCCGGGGGGAGTCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                 <  2:1205764/98‑1 (MQ=37)
                                                                          gcccAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAg                             <  2:2965614/98‑1 (MQ=255)
                                                                            gcAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCa                           >  2:3371386/1‑101 (MQ=255)
                                                                             gaattatCCCGGGGGGGGGCCCGCGGGGAGGTGGGGCAGAGCCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTTTTTGGCGAGCCGCTCTTCGAGCAc                          <  2:4572232/97‑1 (MQ=255)
                                                                                 tatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                      >  1:2995762/1‑101 (MQ=255)
                                                                                  atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                     >  1:3900335/1‑101 (MQ=255)
                                                                                    ccgggggggACCCCCGGGGGGGCGGGGGGCAGAGCCCCCCGGCCCCCCGGGGGCCGAGCCCCCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                   <  1:2664929/98‑1 (MQ=255)
                                                                                    cccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                   >  1:5056834/1‑101 (MQ=255)
                                                                                     cgggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                  <  1:212959/99‑1 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  1:1280373/1‑101 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  2:4258744/1‑101 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  1:2211127/1‑101 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  2:1749286/1‑101 (MQ=255)
                                                                                        gggggAGCCCGGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCGGAGCGCTCAGGTGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCa               <  2:1286200/101‑1 (MQ=255)
                                                                                         ggggGGTCCCGCGGGGGGGGGGGGCAGACCCCCCGTGCCCCCGGGGGGCCGGGCCCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc              <  1:1433074/101‑1 (MQ=255)
                                                                                         ggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc              >  1:4304627/1‑101 (MQ=255)
                                                                                         ggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc              >  1:1729991/1‑101 (MQ=255)
                                                                                         ggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc              >  1:3838580/1‑101 (MQ=255)
                                                                                             aGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg          >  2:4011550/1‑101 (MQ=255)
                                                                                               tCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTTTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg        <  1:5686150/101‑1 (MQ=255)
                                                                                               ccccGCGGGGCCGGGGGGCAGACCCCCCCTCCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg        <  2:4160597/100‑1 (MQ=255)
                                                                                                cccGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgggg       >  2:3415351/1‑101 (MQ=255)
                                                                                                  cgcgGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCTAGCGCTCAGGCGTTTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc     <  1:1882662/101‑1 (MQ=255)
                                                                                                  cgcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc     <  2:2265500/101‑1 (MQ=255)
                                                                                                  cgcgGGGACGGGGGACAGACCCCCCCTCCCCCCCTGGGGCCGAGCGCTCGGGGGTTTTGGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc     <  2:527782/101‑1 (MQ=255)
                                                                                                    gggggGCGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTTTTTGGCGAGCCGATCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   <  1:5762661/100‑1 (MQ=255)
                                                                                                    cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGTGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgc   >  2:5739135/1‑100 (MQ=255)
                                                                                                    cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   >  1:4006173/1‑101 (MQ=255)
                                                                                                    cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   <  2:504207/101‑1 (MQ=255)
                                                                                                    cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt   >  2:3778151/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGAT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG