Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,799 G→C 3.6% intergenic (‑102/+61) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7990G→C 3.6% 2.4 ‑0.4 ‑0.0138intergenic (‑102/+61)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (61/71):  total (65/73)
Fisher's exact test strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 212 total aligned reads displayed.

GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901
                                                                                                       |                                                                                                           
gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttg                                                                                                             <  1:147042/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt                                                                                                        <  1:1196832/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt                                                                                                        <  1:5172979/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt                                                                                                        <  2:4663039/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt                                                                                                        <  1:312679/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGAGTTGGGGCGAG.AGT.AAAGGgg.gttgc.ttt                                                                                                        >  2:4113304/1‑101 (MQ=255)
     gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.tttt                                                                                                       <  2:1046667/101‑1 (MQ=255)
      aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAG.A.G.AAAAGGGGTTTGG.ttttt                                                                                                      >  1:1005856/1‑100 (MQ=255)
      aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTa                                                                                                      <  1:4188290/101‑1 (MQ=255)
        ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATc                                                                                                    <  1:2284241/101‑1 (MQ=255)
          tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGATTTCGGGGAAA.G.T.AAAAGGGCTTTTG.ttttttttt                                                                                                  >  2:605521/1‑96 (MQ=255)
             acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTCGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTcc                                                                                               <  1:4266590/101‑1 (MQ=255)
              cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTTGGGAAG.A.C.AAAGGGGGGTTGT.TTTTTTTTTTtcc                                                                                              >  1:3398320/1‑101 (MQ=255)
              cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGAA.A.AAAGGGGTTTTGT.TTTTTTTTTTcc                                                                                               >  1:2545048/1‑101 (MQ=255)
              cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTccc                                                                                              <  2:2084486/101‑1 (MQ=255)
                gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCa                                                                                            <  2:2249901/101‑1 (MQ=255)
                 cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCaa                                                                                           <  2:4539411/101‑1 (MQ=255)
                 cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCaa                                                                                           <  2:1473860/101‑1 (MQ=255)
                   cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat                                                                                         <  1:1099154/101‑1 (MQ=255)
                   cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat                                                                                         <  2:670569/101‑1 (MQ=255)
                   cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat                                                                                         <  1:2449382/101‑1 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAG.A.G.AAAAGGGGGTTGG.TTTTTTTTTTCCCCaaaaa                                                                                        >  1:5719132/1‑99 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAG.A.T.AAAAGGGGTTTTC.TTTTTTTTTTTCCCCAata                                                                                        >  1:2234420/1‑101 (MQ=37)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAata                                                                                        <  2:2639923/101‑1 (MQ=255)
                     aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAAGGGGTTTGC.TTTTTTTTTTTCCCAAatat                                                                                       >  2:3319628/1‑101 (MQ=255)
                      ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc                                                                                      <  2:3159509/101‑1 (MQ=255)
                      ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc                                                                                      <  1:55675/101‑1 (MQ=255)
                      ccAGCTTTCAAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc                                                                                      <  1:1279130/101‑1 (MQ=255)
                       cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAG.A.A.AAAAGGGGTTTGCTTTTTTTTTTTCCCCaaaattc                                                                                      >  1:4289374/1‑97 (MQ=255)
                       cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATcc                                                                                     <  2:4434519/101‑1 (MQ=255)
                        aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc                                                                                    <  2:4877002/101‑1 (MQ=255)
                        aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc                                                                                    <  1:4507855/101‑1 (MQ=255)
                        aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc                                                                                    <  2:124460/101‑1 (MQ=255)
                         gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCTTTTGGGGCAAG.A.TAAAAGGGGTTTTCC.TTTTTTTTTTTCCCaatttcccg                                                                                    >  1:3573256/1‑94 (MQ=255)
                          cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCgg                                                                                  <  2:4921705/101‑1 (MQ=255)
                          cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCgg                                                                                  <  2:2552255/101‑1 (MQ=255)
                              cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAG.A.T.AAAAGGGCTTTGC.TTTTTTCTTTTCCCCAATATCCCgggggg                                                                              >  2:4414719/1‑100 (MQ=37)
                              cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t                                                                            <  2:2413711/101‑1 (MQ=255)
                              cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t                                                                            <  2:2338883/101‑1 (MQ=255)
                              cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t                                                                            <  1:5525741/101‑1 (MQ=255)
                               gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..Tg                                                                           <  1:2642433/101‑1 (MQ=255)
                                aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGc                                                                          <  2:5637183/101‑1 (MQ=255)
                                 aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCGAG.A.A.AAAAGGGCTTTGCTTTTTTTTTTTCCCCAAAATTCCCgggggggg                                                                            >  1:381717/1‑98 (MQ=255)
                                  aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.A.T.AAAGGGGGGTTGT.TTTTTTTTTTTCCCCAATATCCCcggggggggg                                                                          >  2:509282/1‑96 (MQ=255)
                                   gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..ggg                                                                     <  1:5763491/101‑1 (MQ=255)
                                    aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAGGGGGTTTGG.TTTTTTTTTTTCCCACATATACCCGGGG..GGC..gggg                                                                    >  1:5153494/1‑101 (MQ=255)
                                    aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATAATTGGGGCAAG.A.T.AAAGGGGCCTTGC.TTTTATCTTTCTCCAAACATCCCCGGGG..GGC..ggcg                                                                    >  2:5744536/1‑99 (MQ=25)
                                      aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTGGGGGAAG.A.T.AAAGGGGGTTTGC.TTTTTTCTTTCCCCAAATATCCCGGGGG..GGC..GGGGGc                                                                  >  1:5432536/1‑101 (MQ=38)
                                      aGGAATCCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGc                                                                  <  2:1758545/101‑1 (MQ=255)
                                      aGGAATCCGGCCTGCGGCCGATCCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGTTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGc                                                                  <  1:1354403/101‑1 (MQ=255)
                                       ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.A.AAAAGGGGGTTGC.TTTTTTTTTTTCCCAAATATCCCCGGGG..GGG..ggggggg                                                                 >  1:3975066/1‑99 (MQ=255)
                                        gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAAGGGGTTTTC.TTTTTTCTTTTCCCCAATATACCCGGGG..GGC..GGGGGGag                                                                >  2:5055449/1‑101 (MQ=255)
                                         aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCaga                                                               <  1:251233/101‑1 (MQ=255)
                                          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCagag                                                              <  2:4305019/101‑1 (MQ=255)
                                           ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAG.A.T.AAAGGGGCTTTTC.TTTTTTCTTTTCCCCAATATCCCCGGGG..TGG..ggggggcgagg                                                             >  2:408253/1‑95 (MQ=25)
                                            gcgcccTGGGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAGGGGGTTTTGT.TTTTTTCTTCCCCAAAATATCCCGGGGG..TGC..GGGGGCAGAGcc                                                            <  2:1966955/97‑1 (MQ=21)
                                              ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAG.A.T.AAAGGGGCTTTGC.TTTTTTCTTTTCCCAAATATCCCGGGGG..GGC..GGGGGGAGAGcccc                                                          >  2:1512005/1‑101 (MQ=25)
                                              ggCCTGCGGCCGATGCCTCCGGCAGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGcccc                                                          <  2:5699008/101‑1 (MQ=255)
                                               gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGccccc                                                         <  2:518088/101‑1 (MQ=255)
                                                 tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.ACCCATCCC.TTTCATCTTGCCCCAAATATCCCGGGGG..GCCCGGGGGGCAGCGcccccc                                                        <  1:3481020/94‑2 (MQ=25)
                                                 cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCcgc                                                       >  2:1691109/1‑101 (MQ=255)
                                                 cTGCGCCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC..TTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCcgcc                                                      <  2:5371227/101‑2 (MQ=255)
                                                    ctttcGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.GCCCATCCC.TTTAATCTTGCCCCAAATATCCCGGGGG..ACCCGGGGGGCAGCGccccccgcc                                                     <  2:1873158/97‑5 (MQ=21)
                                                     ggCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGGTTTGC.TTTTATCTTTCCCCAAAAATCCCGGGGG..GGC..GGGGGGAGAGCCCCcgccgcc                                                   >  2:760795/1‑97 (MQ=25)
                                                      ttcGATGCCTCCGGCGGGGATATTTTGGGCAAG.A.T.GAA.AACCATCCC.CTTCATCTTGCCCCCAATATCCCGGGGG..GCCCGGGGGGCAGCGcccccccccac                                                   >  2:690298/3‑95 (MQ=21)
                                                        cGATGCCTCCGGCGGGGAAATTTTGGGCAAG.A.T.AAAGGGGGTTTTG.TTTTATCTTTCCCCAAATATCCCGGGGG..GGC..GGGGGCAGAGCCCCcgccgccacg                                                >  2:5531468/1‑94 (MQ=21)
                                                         gATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.AACCATCCC.TTTCATCTTTCCCCAAATATCCCGGGGGGCCCC..GGGGGCAGCGcccccccccccctc                                                >  2:4451393/1‑92 (MQ=18)
                                                                ccGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCC.C.CCGCCCCGGGTCAcgc                                      >  2:3526761/1‑101 (MQ=18)
                                                                   gcggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                   >  1:4692927/1‑101 (MQ=255)
                                                                   gcggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                   >  1:5236214/1‑101 (MQ=255)
                                                                    cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                  >  1:328963/1‑101 (MQ=255)
                                                                    cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                  >  2:3913489/1‑101 (MQ=255)
                                                                    cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                  >  1:5782886/1‑101 (MQ=255)
                                                                          tatTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                            >  1:2301844/1‑101 (MQ=255)
                                                                           atTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                           >  2:2759915/1‑101 (MQ=255)
                                                                           atTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                           >  2:4321621/1‑101 (MQ=255)
                                                                            tttGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                          >  1:48469/1‑101 (MQ=255)
                                                                            tttGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                          >  2:1681168/1‑101 (MQ=255)
                                                                               ggggCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                       >  2:3468972/1‑101 (MQ=255)
                                                                                gggCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                      >  1:649680/1‑101 (MQ=255)
                                                                                   cAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                   >  2:1583243/1‑101 (MQ=255)
                                                                                    gggaaaaaaaaGGGGTCTTGT.TTTTTTTTTTCCCCAAA.ATCCCCGGGG..GGT..GGGGGGGAAGACCCCCAGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                      <  1:1939306/93‑1 (MQ=255)
                                                                                    aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:5484766/1‑101 (MQ=255)
                                                                                    aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  2:101627/1‑101 (MQ=255)
                                                                                    aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:2337035/1‑101 (MQ=255)
                                                                                     aG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                 >  2:2197728/1‑101 (MQ=255)
                                                                                        a.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa               >  1:5598530/1‑101 (MQ=255)
                                                                                          t.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCGCGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  1:4965843/1‑101 (MQ=255)
                                                                                          t.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  2:5709579/1‑101 (MQ=255)
                                                                                            aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgc             >  1:4486715/1‑101 (MQ=255)
                                                                                                gggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga         >  1:1651199/1‑101 (MQ=255)
                                                                                                 ggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac        >  1:534776/1‑101 (MQ=255)
                                                                                                  gctttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg       >  1:4196535/1‑101 (MQ=255)
                                                                                                    tttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat     >  1:5105610/1‑101 (MQ=255)
                                                                                                    tttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat     >  1:1773478/1‑101 (MQ=255)
                                                                                                     ttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata    >  1:284711/1‑101 (MQ=255)
                                                                                                      tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat   >  1:3032641/1‑101 (MQ=255)
                                                                                                      tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat   >  2:3002694/1‑101 (MQ=255)
                                                                                                      tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat   >  1:938071/1‑101 (MQ=255)
                                                                                                       gc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc  >  1:831176/1‑101 (MQ=255)
                                                                                                       |                                                                                                           
GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG