Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,459 C→G 100% G470G (GGC→GGG RCAP_rcc03391 → type I secretion system ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,4590C→G100.0% 688.8 183G470G (GGC→GGGRCAP_rcc03391type I secretion system ATPase
Reads supporting (aligned to +/- strand):  new base (90/93):  ref base (0/0):  total (90/93)

Only 100 of 213 total aligned reads displayed.

CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556
                                                                                                  |                                                                                                 
cccgcgaccatCGTGGACGCCGCCAAACGGGCGGCCGCTCATCCCCCGATCCCCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 <  1:438749/92‑1 (MQ=255)
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 >  1:786100/1‑101 (MQ=255)
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGAATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 >  2:3088078/1‑101 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcgggt                                                                                              >  2:3750869/1‑99 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGct                                                                                              >  1:4613777/1‑101 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGAGATATGACACCCGGGTTTCGCAGGCGGGcgggcggcc                                                                                              >  1:4942239/1‑100 (MQ=255)
     gatgatCGTCGATGCCGCGAAGCGGGCGGCGGCGCGTCAGCTGATCCTCGATCTGCCCGAGTGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctct                                                                                            <  2:2330260/101‑1 (MQ=255)
       tgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcg                                                                                          >  1:3447438/1‑101 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                         <  2:212526/101‑1 (MQ=255)
          tcgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGTGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGGTCTcgggc                                                                                       >  2:2835349/1‑101 (MQ=255)
          tcgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggc                                                                                       >  2:2511645/1‑101 (MQ=255)
           cgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcg                                                                                      >  1:1855914/1‑101 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     <  2:5149816/101‑1 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgggg                                                                                   >  2:4595827/1‑100 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGCTATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgggga                                                                                  >  2:3311627/1‑99 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  <  2:4406983/101‑1 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  <  2:4118011/101‑1 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  >  1:4517247/1‑101 (MQ=255)
                aTGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTCGCGCGGGCAg                                                                                 >  1:4991747/1‑101 (MQ=255)
                  gCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGAt                                                                               <  1:119051/101‑1 (MQ=255)
                  gCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGAt                                                                               <  2:1837053/101‑1 (MQ=255)
                     gcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCa                                                                            <  2:2482955/101‑1 (MQ=255)
                     gcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCa                                                                            >  1:3753240/1‑101 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                           >  2:371862/1‑101 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                           >  1:2256028/1‑101 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                           <  1:4460009/101‑1 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCCGATCCAgc                                                                          >  2:2276598/1‑101 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgc                                                                          <  1:2819004/101‑1 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgc                                                                          <  2:591864/101‑1 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         <  1:1690206/101‑1 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         >  2:1693644/1‑101 (MQ=255)
                         aaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACAACCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcgc                                                                        <  1:4043321/101‑1 (MQ=255)
                          aCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGcgcg                                                                       >  2:3169467/1‑101 (MQ=255)
                            gggCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGGTCTCGGGCGGGCAGATCCAGcgcgcc                                                                     >  2:766942/1‑99 (MQ=255)
                              gcggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCgg                                                                   <  2:4524406/101‑1 (MQ=255)
                              gcggcgccgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCgg                                                                   <  2:611521/101‑1 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGcc                                                                 >  2:1049536/1‑101 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGcc                                                                 >  1:1943178/1‑101 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGcc                                                                 <  1:68711/101‑1 (MQ=255)
                                    gcgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGc                                                             >  2:1951857/1‑101 (MQ=255)
                                    gcgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGc                                                             <  2:5401645/101‑1 (MQ=255)
                                     cgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGcc                                                            >  1:5351068/1‑101 (MQ=255)
                                     cgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGcc                                                            <  2:4711113/101‑1 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGGAGATCCAGCGCGTCGGGCTGGccc                                                           >  2:3626529/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           >  1:1421186/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCGCTCGGGCGGGCAGATCCAGGGCGTCGGCCGCGccc                                                           >  1:1032664/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                          >  2:4019214/1‑101 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGCCCgg                                                         >  1:5022984/1‑101 (MQ=255)
                                          cAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGGGCGGCGGCCTGGCCCGGgc                                                       >  1:1950696/1‑101 (MQ=255)
                                          cAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgc                                                       <  1:365354/101‑1 (MQ=255)
                                           aGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCCGATCCAGCGCGTCGGCCTGGCCCggggg                                                      >  1:1733559/1‑99 (MQ=255)
                                           aGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgcg                                                      >  2:4143435/1‑101 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgcgc                                                     <  2:2173223/101‑1 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   >  1:1233995/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   >  2:4694332/1‑101 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGCCCGGGCGCTTTa                                                 >  1:3977288/1‑101 (MQ=255)
                                                  ccacGATCTGCCGGAGGGATATGACGCCGGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGACCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACg                                               <  2:3114312/98‑1 (MQ=38)
                                                  ccTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACg                                               >  1:5491209/1‑101 (MQ=255)
                                                  ccTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGCCGGCCTGGCCCGGGCGCTTTACg                                               >  1:3611002/1‑101 (MQ=255)
                                                          tGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCg                                       >  2:2755183/1‑101 (MQ=255)
                                                          tGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCg                                       <  2:971461/101‑1 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgg                                      <  1:1518503/101‑1 (MQ=255)
                                                            cccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGt                                     <  2:2378844/101‑1 (MQ=255)
                                                            cccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCACCGCGTCGGCCTGGGCCGGGGGCTTTGCGGCAAccccgg                                     >  2:2607901/1‑98 (MQ=255)
                                                            cccGAGGGATATGACACCCGGGGTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGt                                     <  1:3311827/101‑1 (MQ=255)
                                                             ccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCCGGCCCGGGCGCTTTACGGCAACCCGGtg                                    >  2:4642124/1‑101 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgc                                   <  1:2601955/101‑1 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgc                                   <  1:5276137/101‑1 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGAGCGTCGGCCTGGGCCGGGGGCTTTACGGCAACCCCGtgc                                   >  2:2294324/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  >  1:2956690/1‑101 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                 <  2:5710958/101‑1 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCCGtgctg                                 >  2:5029549/1‑101 (MQ=255)
                                                                 gggATATGACACCCGGGCTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATTCCGCCCGCCGGCCCCGCCCGGGCGCGTTACCGCAACCCggaggccc                                >  2:2058049/1‑95 (MQ=255)
                                                                  ggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTgctgct                               >  2:854348/1‑101 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGt                            <  1:5210211/101‑1 (MQ=255)
                                                                      atGACACCCGGGTTTCGCAGGCGGGCGGGCGGCCCTCGGGCGGGCAGATCCAGCGCGGCGGCCCGGCCCGGGCGCTTTCCGGCAACCCCGTGCAGCCGGTg                           >  2:1731856/1‑101 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGGCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                        >  2:5297236/1‑101 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                        <  2:3959385/101‑1 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGGAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      >  2:1964461/1‑101 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      >  2:5519358/1‑101 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      <  2:2571592/101‑1 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTGGTGCTcga                      >  1:4760239/1‑101 (MQ=255)
                                                                            cccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCATCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgac                     <  1:1010178/101‑1 (MQ=255)
                                                                            cccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgac                     <  1:764416/101‑1 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  >  1:3855025/1‑101 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  2:915773/101‑1 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  1:2511787/101‑1 (MQ=255)
                                                                                ggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGc                 <  2:4550997/101‑1 (MQ=255)
                                                                                ggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGc                 >  1:4054308/1‑101 (MQ=255)
                                                                                 gTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGcc                >  1:4824768/1‑101 (MQ=255)
                                                                                     cGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAAt            >  1:4340750/1‑101 (MQ=255)
                                                                                      gCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  2:3390940/101‑1 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          >  1:5089063/1‑101 (MQ=255)
                                                                                        aGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg         >  1:2316093/1‑101 (MQ=255)
                                                                                         ggcgggcgGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGa        >  1:5456987/1‑101 (MQ=255)
                                                                                          gcgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGaa       <  2:3454338/101‑1 (MQ=255)
                                                                                          gcgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGaa       <  2:300507/101‑1 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc     >  2:1534550/1‑101 (MQ=255)
                                                                                              gcgggCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtctc   <  1:5767320/101‑1 (MQ=255)
                                                                                              gcgggCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtctc   <  2:4875615/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG