Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,383 G→A 100% R246H (CGC→CAC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,3830G→A100.0% 1215.6 316R246H (CGC→CAC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (144/170):  ref base (0/0):  total (145/171)

Only 100 of 327 total aligned reads displayed.

CGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCGCAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATATTTGGGGC  >  NC_014034/367285‑367480
                                                                                                  |                                                                                                    
cacccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACa                                                                                                    >  1:3354992/3‑101 (MQ=255)
 acccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGCTCGACGAGCAGAACAACCCGCATGACTTCTTCCACaa                                                                                                   >  1:4952806/2‑101 (MQ=255)
 acccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACaa                                                                                                   >  1:4549032/2‑101 (MQ=255)
  cccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAAc                                                                                                  <  1:2973329/101‑1 (MQ=255)
   ccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAAcc                                                                                                 >  2:4404186/1‑101 (MQ=255)
   ccATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAAcc                                                                                                 <  2:292463/101‑1 (MQ=255)
     aTGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCg                                                                                               <  2:5345120/101‑1 (MQ=255)
      tGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGc                                                                                              >  1:3233664/1‑101 (MQ=255)
       gagaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCa                                                                                             <  2:2966555/101‑1 (MQ=255)
       gagaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCa                                                                                             >  1:3666985/1‑101 (MQ=255)
       gagaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCa                                                                                             <  1:4822151/101‑1 (MQ=255)
        agaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCaa                                                                                            >  2:3620554/1‑101 (MQ=255)
        agaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCaa                                                                                            <  1:3067303/101‑1 (MQ=255)
        agaTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCaa                                                                                            <  1:5415799/101‑1 (MQ=255)
          aTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATc                                                                                          <  1:4057799/101‑1 (MQ=255)
           tGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAAGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCg                                                                                         >  2:5002977/1‑101 (MQ=255)
            gggCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCgg                                                                                        <  2:2090728/101‑1 (MQ=255)
              gCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAg                                                                                      <  2:822106/101‑1 (MQ=255)
               cTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGc                                                                                     <  2:1258506/101‑1 (MQ=255)
                tttGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCTCAACCCGCAATCGGAGCg                                                                                    >  2:5504897/1‑101 (MQ=255)
                tttGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCg                                                                                    <  2:4742776/101‑1 (MQ=255)
                tttGCCCAGGCGGGGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCg                                                                                    <  1:5297696/101‑1 (MQ=255)
                 ttGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCgg                                                                                   <  2:3125328/101‑1 (MQ=255)
                  tGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGa                                                                                  <  2:4556533/101‑1 (MQ=255)
                   gCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGAc                                                                                 <  1:48784/101‑1 (MQ=255)
                    cccAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACg                                                                                <  1:573262/101‑1 (MQ=255)
                    cccAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACg                                                                                >  2:1865265/1‑101 (MQ=255)
                     ccAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGa                                                                               <  1:1353686/101‑1 (MQ=255)
                       aGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAg                                                                             >  1:4741582/1‑101 (MQ=255)
                         gCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCa                                                                           <  2:1486807/101‑1 (MQ=255)
                         gCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCa                                                                           <  1:1011528/101‑1 (MQ=255)
                          cGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAg                                                                          >  2:84071/1‑101 (MQ=255)
                           ggTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGt                                                                         >  2:5702973/1‑101 (MQ=255)
                             tGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTc                                                                       >  1:5359876/1‑101 (MQ=255)
                              ggCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTcc                                                                      >  2:2627300/1‑101 (MQ=255)
                              ggCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTcc                                                                      <  1:144168/101‑1 (MQ=255)
                               gCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCt                                                                     <  1:1909742/101‑1 (MQ=255)
                                cGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTg                                                                    >  2:2772816/1‑101 (MQ=255)
                                 gagcctggTGATCTTGACGGCGGGCGGGCAGGTCGTCGAGCAGAACACCCCGCGCGACTTCTTCCACAACCCGCAACCGGAGCGGACGAAACAGTTCCTGa                                                                   <  2:194744/95‑1 (MQ=255)
                                   aCCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGc                                                                 >  1:1312259/1‑101 (MQ=255)
                                    ccGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGcc                                                                <  2:3301376/101‑1 (MQ=255)
                                    ccGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGcc                                                                <  1:2575962/101‑1 (MQ=255)
                                     cGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCa                                                               >  1:2144166/1‑101 (MQ=255)
                                     cGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCa                                                               >  2:3973845/1‑101 (MQ=255)
                                         tGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGAtt                                                           <  1:5551925/101‑1 (MQ=255)
                                         tGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGAtt                                                           >  1:2950772/1‑101 (MQ=255)
                                          gATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTc                                                          >  1:2613020/1‑101 (MQ=255)
                                           aTCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCt                                                         <  2:4531621/101‑1 (MQ=255)
                                             cTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTgg                                                       <  2:1041285/101‑1 (MQ=255)
                                                cATGGCCGACGGGCAGATCGTCGAGCAAAACAACCAGCATGACTTCTTCCACAACCCGCAATCGGAGCGCACGAAGAAGTTCCTGAGAAAGATTCTGGGGc                                                    >  2:5051003/1‑101 (MQ=255)
                                                 aTGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCa                                                   >  2:2653814/1‑101 (MQ=255)
                                                  tGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCAt                                                  <  2:2291690/101‑1 (MQ=255)
                                                    gCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTg                                                <  1:1542626/101‑1 (MQ=255)
                                                     ccGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGa                                               <  1:17952/101‑1 (MQ=255)
                                                       gACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.g                                            >  2:2006335/1‑101 (MQ=255)
                                                          gggCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.gggg                                         >  1:2059475/1‑100 (MQ=255)
                                                          gggCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.gggg                                         >  1:650850/1‑100 (MQ=255)
                                                           ggCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.ggggg                                        >  2:4101884/1‑99 (MQ=255)
                                                           ggCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.ggggg                                        >  2:1566497/1‑99 (MQ=255)
                                                            gCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTgg                                       <  2:4330198/101‑1 (MQ=255)
                                                             cAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGTAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTggg                                      <  2:4264029/101‑1 (MQ=255)
                                                             cAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTggg                                      <  2:3910853/101‑1 (MQ=255)
                                                              aGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTgggg                                     <  1:4090446/101‑1 (MQ=255)
                                                               gATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTggggg                                    <  1:833153/101‑1 (MQ=255)
                                                                aTCGTCGAGCAGAACAACCCGCATGACTTCTTCCTCAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGgc                                   <  1:5505189/101‑1 (MQ=255)
                                                                aTCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGgc                                   <  1:2878689/101‑1 (MQ=255)
                                                                aTCGTCGAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGgc                                   <  2:4060084/101‑1 (MQ=255)
                                                                  cgtcgAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGgggc                                 >  2:2240178/1‑101 (MQ=255)
                                                                   gtcgAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCt                                <  2:2158335/101‑1 (MQ=255)
                                                                    tcgAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGACTCTGGGGCATTGAA.GGGTGGGGGCGCTg                               <  2:5461531/101‑1 (MQ=255)
                                                                     cgAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGggggggggcgc                              >  1:223639/1‑95 (MQ=255)
                                                                      gAGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTC..cc                           <  1:4273782/101‑1 (MQ=255)
                                                                       aGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..gcc                          >  2:3569070/1‑101 (MQ=255)
                                                                       aGCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..ccc                          <  2:5619373/101‑1 (MQ=255)
                                                                        gCAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGCGCCG..cccc                         >  2:658910/1‑101 (MQ=255)
                                                                         cAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..ccccc                        >  2:5084900/1‑101 (MQ=255)
                                                                         cAGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGCGG..cgccc                        >  2:5188620/1‑101 (MQ=255)
                                                                          aGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCca                       <  2:3451690/101‑1 (MQ=255)
                                                                          aGAACAACCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGCTG..gccccc                       >  2:767959/1‑100 (MQ=255)
                                                                            aacaccCCGCATGACTTCTCCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCcaca                     <  2:891455/101‑1 (MQ=255)
                                                                             acaacCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGCGGTG..CCCCCacac                    >  2:1681260/1‑101 (MQ=255)
                                                                              aaacCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACAcc                   <  2:1628333/100‑1 (MQ=255)
                                                                               aacCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACAccc                  <  1:3569287/101‑1 (MQ=255)
                                                                               aacCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGAAGTTCCTCAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGAGCGG..CCCCCGCAccc                  >  1:1166531/1‑101 (MQ=255)
                                                                                acCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGGCG..GCCCCCCAcccc                 >  2:2414419/1‑101 (MQ=255)
                                                                                acCCGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCT.GGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACAccccc                <  2:1339344/101‑1 (MQ=255)
                                                                                 cccGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACAccccc                <  1:1673438/101‑1 (MQ=255)
                                                                                  cggcatctcttcgtCCACAACCCGTAATCGGAGCGGACGAGGCATTTCCTGGGCCAGATTCTGGGGCATGGAA.GGGTGGGGGCGCTG..CCCCCACTCCCCCg               <  2:5380119/93‑1 (MQ=255)
                                                                                  ccGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCg               <  2:3677739/101‑1 (MQ=255)
                                                                                   cGCATGACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGCCGGGGGCGCTG..CCCCCCCACCCCCgg              >  2:4790817/1‑101 (MQ=255)
                                                                                        gACTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGatat         <  1:4404453/101‑1 (MQ=255)
                                                                                         aCTTCTTCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGGGG..GCCCCCCCcccccccggtatt        >  1:5646923/1‑93 (MQ=255)
                                                                                          cttcttCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAAGGGGGGGGGGGGCGG..CCCCCCCCCCCCCgcgggatt        >  1:4626182/1‑94 (MQ=255)
                                                                                          cttcttCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATAttt       <  1:3940552/101‑1 (MQ=255)
                                                                                          cttcttCCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATAttt       <  1:2522111/101‑1 (MQ=255)
                                                                                           ttcttcCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGGGGGCCCCCCACCCCCCCGGGATTtt        >  2:4939723/1‑101 (MQ=255)
                                                                                            tcttcCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATATTTgg     <  1:906072/101‑1 (MQ=255)
                                                                                            tcttcCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGGGGTG..GCCCCCCACCCCCcggggatagaa     >  2:2525231/1‑93 (MQ=255)
                                                                                            tcttcCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGGGGGGGCGCTG..GCCCCACCCCCCCGGGATCttcag     >  2:2836059/1‑98 (MQ=255)
                                                                                               tcCACAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATATTTGGGGc  <  1:5173135/101‑1 (MQ=255)
                                                                                                  |                                                                                                    
CGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCGCAACCCGCAATCGGAGCGGACGAAGCAGTTCCTGAGCCAGATTCTGGGGCATTGAA.GGGTGGGGGCGCTG..CCCCCACACCCCCGGGATATTTGGGGC  >  NC_014034/367285‑367480

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG