Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 419,739 C→A 100% T591N (ACC→AAC)  RCAP_rcc00388 → OmpA/MotB domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034419,7390C→A100.0% 981.9 255T591N (ACC→AAC) RCAP_rcc00388OmpA/MotB domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (138/116):  ref base (0/0):  total (139/116)

Only 100 of 280 total aligned reads displayed.

TTCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGACCCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCCGCGCCC  >  NC_014034/419641‑419839
                                                                                                  |                                                                                                    
ttCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAacc                                                                                                    <  2:4598988/101‑1 (MQ=255)
ttCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAacc                                                                                                    >  2:3248031/1‑101 (MQ=255)
ttCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAacc                                                                                                    >  2:1411748/1‑101 (MQ=255)
  ccGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCgg                                                                                                  >  1:757801/1‑101 (MQ=255)
   cgcgcgCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGgc                                                                                                 <  1:4815220/101‑1 (MQ=255)
    gcgcgcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGgcg                                                                                                <  1:5009417/101‑1 (MQ=255)
      gcgcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCa                                                                                              <  1:3877758/101‑1 (MQ=255)
       cgcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCAt                                                                                             <  2:3782570/101‑1 (MQ=255)
        gcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATc                                                                                            >  1:4241980/1‑101 (MQ=255)
         ccGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCg                                                                                           <  1:3012966/101‑1 (MQ=255)
          cGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGa                                                                                          <  1:2779455/101‑1 (MQ=255)
           gggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGaa                                                                                         >  2:5428500/1‑101 (MQ=255)
           gggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGaa                                                                                         <  2:5683413/101‑1 (MQ=255)
            ggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAAt                                                                                        >  1:55332/1‑101 (MQ=255)
             gTGCTGACGCGCAACCTGACGCCCAAGGGCTATGTCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAAtt                                                                                       <  2:2508501/101‑1 (MQ=255)
             gTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGTCAAGGGCCGCGAGGCGAACCGGCGCATCGAAtt                                                                                       >  1:4984991/1‑101 (MQ=255)
             gTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAAtt                                                                                       >  1:4442556/1‑101 (MQ=255)
               gCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCg                                                                                     >  1:430646/1‑101 (MQ=255)
               gCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCg                                                                                     <  2:1966268/101‑1 (MQ=255)
                cTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCACCGAATTCgg                                                                                    >  2:2785631/1‑100 (MQ=255)
                 tGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGtg                                                                                   >  2:3992729/1‑101 (MQ=255)
                    cgcgCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGtgctg                                                                                >  1:3996609/1‑101 (MQ=255)
                      cgcAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTgctgct                                                                              <  2:5049298/101‑1 (MQ=255)
                       gcAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGctgctg                                                                             <  1:5313903/101‑1 (MQ=255)
                       gcAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGctgctg                                                                             >  1:1711152/1‑101 (MQ=255)
                       gcAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGctgctg                                                                             >  1:4749305/1‑101 (MQ=255)
                         aaCCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGa                                                                           <  1:4350682/101‑1 (MQ=255)
                          ttccgatctccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGAt                                                                          <  2:925263/92‑1 (MQ=255)
                           ccTGACGCCCAAGGGCTATGGCTAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATg                                                                         <  2:947544/101‑1 (MQ=255)
                           ccTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATg                                                                         <  2:1741249/101‑1 (MQ=255)
                              gACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCa                                                                      >  2:4904116/1‑101 (MQ=255)
                               aCGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGATCCGGCGCATCGAATTCGTGCTGCTGGATGCCaa                                                                     >  1:4940065/1‑101 (MQ=255)
                                 gCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGc                                                                   <  1:4271237/101‑1 (MQ=255)
                                  cccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGcc                                                                  >  2:5598591/1‑101 (MQ=255)
                                  cccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGcc                                                                  <  1:452333/101‑1 (MQ=255)
                                   ccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGAtagatcggaa                                                                 >  1:925339/1‑92 (MQ=255)
                                   ccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCg                                                                 >  2:4227079/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                               <  1:86380/101‑1 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                               >  1:3731160/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                               <  1:2325218/101‑1 (MQ=255)
                                         gCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggg                                                           <  2:3372245/101‑1 (MQ=255)
                                         gCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggg                                                           <  2:54649/101‑1 (MQ=255)
                                          ccgatctgAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTggggg                                                          <  1:3484818/94‑1 (MQ=255)
                                           tATGGCGAAAGCCAGCCCAGCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggggg                                                         <  1:3248231/101‑1 (MQ=255)
                                           tATGGCGAAACCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggggg                                                         <  2:4720358/101‑1 (MQ=255)
                                            aTGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgc                                                        >  2:648435/1‑101 (MQ=255)
                                            aTGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgc                                                        <  2:1139271/101‑1 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                       <  2:1009551/101‑1 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                       >  2:4529955/1‑101 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                       >  1:2944199/1‑101 (MQ=255)
                                               gCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGcc                                                     >  1:3418384/1‑101 (MQ=255)
                                                ggAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGcca                                                    <  2:4279304/100‑2 (MQ=255)
                                                 gAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCgg                                                   >  1:4175152/1‑101 (MQ=255)
                                                 gAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCgg                                                   >  1:1942816/1‑101 (MQ=255)
                                                 gAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCgg                                                   >  1:5407966/1‑101 (MQ=255)
                                                  aaaGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCggg                                                  <  1:4062528/101‑1 (MQ=255)
                                                   aaGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCACGCCGGTGGGGGGCGCCGGGc                                                 >  1:2584321/1‑101 (MQ=255)
                                                    agccagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCa                                                <  2:429304/101‑1 (MQ=255)
                                                     gccagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAg                                               <  2:1878530/101‑1 (MQ=255)
                                                       cagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgat                                             >  2:3328048/1‑101 (MQ=255)
                                                        agccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatc                                            >  1:1842577/1‑101 (MQ=255)
                                                        agccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatc                                            <  1:1086976/101‑1 (MQ=255)
                                                         gccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatcg                                           <  2:81730/101‑1 (MQ=255)
                                                          cccATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatcga                                          >  1:2790191/1‑101 (MQ=255)
                                                            cATCGCCGAGAATGACACCGACAAGGGCAGCGAGGCGAACCGGCGCATCGAATTCGTGCTGATGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatcgatc                                        >  2:4773436/1‑101 (MQ=255)
                                                              tCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTg                                      <  2:2308672/101‑1 (MQ=255)
                                                                gCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGcc                                    <  1:2461694/101‑1 (MQ=255)
                                                                gCCGAGAATGACACCGACAAGGGCCGCGAGGAGAACCGGCGCAACGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCACCGGGCAGATCGCTCTGcc                                    >  1:5744979/1‑101 (MQ=255)
                                                                   gagaATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGt                                 <  2:757733/101‑1 (MQ=255)
                                                                     gaATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGa                               >  2:121089/1‑101 (MQ=255)
                                                                      aaTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTTGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGAc                              >  1:4119647/1‑101 (MQ=255)
                                                                      aaTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGAc                              >  1:2894883/1‑101 (MQ=255)
                                                                       aTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACg                             >  1:4546955/1‑101 (MQ=255)
                                                                        tgcccccGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACgg                            <  2:496469/96‑1 (MQ=255)
                                                                        tGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACgg                            <  1:3584399/101‑1 (MQ=255)
                                                                         gACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGt                           >  2:3082674/1‑101 (MQ=255)
                                                                         gACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGt                           >  2:2585167/1‑101 (MQ=255)
                                                                          acacCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTg                          <  1:2285744/101‑1 (MQ=255)
                                                                           cacCGACAAGGGCCGGGAGGCGAACCGGCGCATCGAATTCGTGATGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGAAGATCGATCTGCCGGTGACGGTGc                         >  2:689228/1‑101 (MQ=255)
                                                                           cacCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGc                         <  2:1711053/101‑1 (MQ=255)
                                                                            acCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGcc                        >  2:3833565/1‑101 (MQ=255)
                                                                               gACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGGGACGGTGCCGGa                     >  2:2867098/1‑101 (MQ=255)
                                                                                ccAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGaa                    <  1:1434042/100‑1 (MQ=255)
                                                                                aCAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGaa                    >  2:1546308/1‑101 (MQ=255)
                                                                                aCAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGaa                    >  2:1103099/1‑101 (MQ=255)
                                                                                 cAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAc                   >  1:5475118/1‑101 (MQ=255)
                                                                                  aaGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAcc                  >  1:1872517/1‑101 (MQ=255)
                                                                                  aaGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAcc                  >  2:4633676/1‑101 (MQ=255)
                                                                                  aaGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAcc                  <  2:2925528/101‑1 (MQ=255)
                                                                                    gggCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAccgc                >  1:1511426/1‑101 (MQ=255)
                                                                                     ggCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAccgcc               <  1:2148580/101‑1 (MQ=255)
                                                                                         ctgAGGCGAACCGGCGCATCGAATTCGGGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAg           <  1:1193097/99‑1 (MQ=255)
                                                                                          cgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGc          >  2:3532698/1‑101 (MQ=255)
                                                                                          cgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGc          >  1:5788823/1‑101 (MQ=255)
                                                                                          cgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGc          <  1:5657659/101‑1 (MQ=255)
                                                                                          cgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGc          >  1:5079472/1‑101 (MQ=255)
                                                                                          cgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGc          <  1:1077996/101‑1 (MQ=255)
                                                                                           gAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGcc         >  1:2552849/1‑101 (MQ=255)
                                                                                            aGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCTGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGccc        <  2:3721147/101‑1 (MQ=255)
                                                                                              gCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCcgc      <  1:671137/101‑1 (MQ=255)
                                                                                                  |                                                                                                    
TTCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGACCCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCCGCGCCC  >  NC_014034/419641‑419839

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG