Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 429,595 T→G 100% R133R (AGG→CGG)  pyrB ← aspartate carbamoyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034429,5950T→G100.0% 1013.8 272R133R (AGG→CGG) pyrBaspartate carbamoyltransferase
Reads supporting (aligned to +/- strand):  new base (127/145):  ref base (0/0):  total (127/145)

Only 100 of 285 total aligned reads displayed.

ACATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCTCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATGC  >  NC_014034/429498‑429692
                                                                                                 |                                                                                                 
aCATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGccc                                                                                                <  1:1357624/101‑1 (MQ=255)
  aTCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGt                                                                                              <  1:2540642/101‑1 (MQ=255)
   tCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTc                                                                                             <  1:2708010/101‑1 (MQ=255)
     gccgcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGc                                                                                           >  2:2402352/1‑101 (MQ=255)
      ccgcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGcc                                                                                          >  2:2490985/1‑101 (MQ=255)
       cgcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGccc                                                                                         <  2:461862/101‑1 (MQ=255)
        gcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCg                                                                                        <  2:580757/101‑1 (MQ=255)
          aaaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCa                                                                                      >  2:3291359/1‑101 (MQ=255)
          aaaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCa                                                                                      >  2:3805723/1‑101 (MQ=255)
           aaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAACGCGCCTGCGTCGGGTGTTCGTCCCGCCCgccccctgcct                                                                                     >  1:5481099/1‑91 (MQ=255)
           aaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCAt                                                                                     <  1:4267125/101‑1 (MQ=255)
           aaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCAt                                                                                     <  1:750829/101‑1 (MQ=255)
           aaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGGTCGTGCCGCCCGTCGCCCGCAt                                                                                     >  2:1189158/1‑101 (MQ=255)
            aTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCAtt                                                                                    >  2:5095155/1‑101 (MQ=255)
            aTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCAtt                                                                                    >  2:4281900/1‑101 (MQ=255)
               gCCGCCGTCAGCCGGTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGAt                                                                                 <  2:2238507/101‑1 (MQ=255)
               gCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGAt                                                                                 >  2:4009432/1‑101 (MQ=255)
                 ccccGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATca                                                                               <  2:2107472/99‑1 (MQ=255)
                 cACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATca                                                                               >  2:3160561/1‑101 (MQ=255)
                   ccGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATcaca                                                                             <  2:3962067/101‑1 (MQ=255)
                     gTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGc                                                                           >  2:1180501/1‑101 (MQ=255)
                      tCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATcacaccc                                                                          >  2:2810653/1‑98 (MQ=255)
                        aGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGcaca                                                                        <  1:3718798/101‑1 (MQ=255)
                           cGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAAtt                                                                     >  1:159093/1‑101 (MQ=255)
                            gCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTc                                                                    <  1:1008720/101‑1 (MQ=255)
                               ggATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCAcc                                                                 <  2:5504795/101‑1 (MQ=255)
                               ggATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCAcc                                                                 >  2:4436248/1‑101 (MQ=255)
                                 aTCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCCCCTGCGTCGGGTGTTCGTGCCGCCCGCCGCCCGCATTGATCACAGCACAATTCACCtt                                                               >  1:3893944/1‑101 (MQ=255)
                                 aTCCGGCCCTTGGCACGCCGGATCGTCAAGGAATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCCCAGCACAATTCACCtt                                                               >  1:5585348/1‑101 (MQ=255)
                                  tCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTc                                                              <  1:3746448/101‑1 (MQ=255)
                                   ccGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCt                                                             <  2:4829161/101‑1 (MQ=255)
                                    cGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCta                                                            >  2:5291434/1‑100 (MQ=255)
                                    cGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTg                                                            >  1:642922/1‑101 (MQ=255)
                                     ggCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTgc                                                           <  1:2656107/101‑1 (MQ=255)
                                        ccTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGcc                                                        <  1:3834362/101‑1 (MQ=255)
                                        ccTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGcc                                                        <  1:3025082/101‑1 (MQ=255)
                                         cTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCa                                                       >  1:5447777/1‑101 (MQ=255)
                                            ggCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCagaa                                                    <  2:1301088/101‑1 (MQ=255)
                                            ggCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGGGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCagaa                                                    >  2:4737706/1‑101 (MQ=255)
                                               aCGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGAt                                                 <  1:5800023/101‑1 (MQ=255)
                                                cGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATc                                                <  2:5406699/101‑1 (MQ=255)
                                                cGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATc                                                >  2:1334527/1‑101 (MQ=255)
                                                 gCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCAGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCg                                               >  2:5737918/1‑101 (MQ=255)
                                                 gCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCACCCGCATTGATCACAGCAGAATTCACCTTCTGCGCCAGAAGATCg                                               >  1:4865087/1‑101 (MQ=255)
                                                  ccGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGa                                              <  2:3718667/101‑1 (MQ=255)
                                                  ccGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGa                                              <  1:804003/101‑1 (MQ=255)
                                                   cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             >  2:104478/1‑101 (MQ=255)
                                                   cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             <  2:83777/101‑1 (MQ=255)
                                                    ggATCTTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACg                                            <  1:3490345/101‑1 (MQ=255)
                                                    ggATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACg                                            >  1:167181/1‑101 (MQ=255)
                                                      aTCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGgc                                          <  1:1390786/101‑1 (MQ=255)
                                                        cGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATcagatcgga                                        >  2:2231085/1‑93 (MQ=255)
                                                        cGTCAAGGCATCCAAAAGCGCCTGCGGCGGGTGTTCGTGCCGCCCGTTGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGgcgc                                        >  2:879201/1‑101 (MQ=255)
                                                            aaGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCgga                                    >  1:2343328/1‑101 (MQ=255)
                                                            aaGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCgga                                    <  1:873933/101‑1 (MQ=255)
                                                             aGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggag                                   <  1:1284462/101‑1 (MQ=255)
                                                             aGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggag                                   >  1:2581856/1‑101 (MQ=255)
                                                               gCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggagga                                 >  1:4469549/1‑101 (MQ=255)
                                                                  tCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAggg                              >  1:3433788/1‑101 (MQ=255)
                                                                   ccAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGa                             >  1:2844589/1‑101 (MQ=255)
                                                                   ccAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGa                             >  1:2799721/1‑101 (MQ=255)
                                                                   ccAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGa                             >  2:5019804/1‑101 (MQ=255)
                                                                   ccAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGAAGGGa                             >  2:234217/1‑101 (MQ=255)
                                                                      aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          >  1:199812/1‑101 (MQ=255)
                                                                      aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          >  1:2273597/1‑101 (MQ=255)
                                                                      aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          >  2:5628641/1‑101 (MQ=255)
                                                                       aaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGcc                         <  2:2745098/101‑1 (MQ=255)
                                                                        aGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCg                        >  2:4022699/1‑101 (MQ=255)
                                                                        aGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCg                        >  1:1553446/1‑101 (MQ=255)
                                                                         gcgcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGc                       <  1:532852/101‑1 (MQ=255)
                                                                         gcgcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGc                       >  2:2984374/1‑101 (MQ=255)
                                                                         gcgcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGc                       <  1:1180582/101‑1 (MQ=255)
                                                                          cgcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGca                      <  1:2402486/101‑1 (MQ=255)
                                                                          cgcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGca                      >  2:1508312/1‑101 (MQ=255)
                                                                           gcCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcac                     <  2:5146537/101‑1 (MQ=255)
                                                                             cTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcacca                   >  1:2183913/1‑101 (MQ=255)
                                                                              tGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcaccac                  <  2:3620138/101‑1 (MQ=255)
                                                                              tGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcaccac                  <  1:5212420/101‑1 (MQ=255)
                                                                              tGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcaccac                  <  1:4009679/101‑1 (MQ=255)
                                                                              tGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcaccac                  <  1:4534349/101‑1 (MQ=255)
                                                                               gCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCaccacc                 <  1:4737987/101‑1 (MQ=255)
                                                                               gCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCaccacc                 >  2:1707921/1‑101 (MQ=255)
                                                                                cGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCAccacca                <  1:104498/101‑1 (MQ=255)
                                                                                 gTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcag               <  1:1825284/101‑1 (MQ=255)
                                                                                  tCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagc              <  1:5096609/101‑1 (MQ=255)
                                                                                  tCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagc              >  2:4069722/1‑101 (MQ=255)
                                                                                   cGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagca             >  2:4183591/1‑101 (MQ=255)
                                                                                    gggTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagcag            <  2:1617801/101‑1 (MQ=255)
                                                                                      gtgtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGAt          <  1:3148910/101‑1 (MQ=255)
                                                                                       tgtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGCGGGATGCCGCACCACCAGCAGATc         >  2:2455171/1‑101 (MQ=255)
                                                                                       tgtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATc         >  1:4380504/1‑101 (MQ=255)
                                                                                         ttCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCgg       >  2:1853767/1‑101 (MQ=255)
                                                                                           cGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGGGGGATGCCGCACCACCAGCAGATCGGGa     >  2:2879942/1‑101 (MQ=255)
                                                                                           cGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCGCCAGCAGATCGGGa     <  2:5646188/101‑1 (MQ=255)
                                                                                           cGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGa     >  2:263969/1‑101 (MQ=255)
                                                                                            gTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGAt    <  2:5253220/101‑1 (MQ=255)
                                                                                            gTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGAt    >  1:5720005/1‑101 (MQ=255)
                                                                                             tGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATg   <  1:1647491/101‑1 (MQ=255)
                                                                                             tGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATg   <  1:954546/101‑1 (MQ=255)
                                                                                             tGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATg   >  2:4529243/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
ACATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCTCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATGC  >  NC_014034/429498‑429692

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG