Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,753 C→G 6.9% A1618A (GCG→GCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,7530C→G 6.9% 31.6 ‑0.5 ‑0.5232A1618A (GCG→GCC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (10/6):  ref base (118/98):  total (128/104)
Fisher's exact test strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.99e-01

Only 100 of 253 total aligned reads displayed.

TCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCG  >  NC_014034/678653‑678853
                                                                                                    |                                                                                                    
tCTGCCCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATcc                                                                                                      <  1:2593448/101‑1 (MQ=255)
 cTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCcg                                                                                                     >  1:5295175/1‑101 (MQ=255)
   gcgcCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCcgcg                                                                                                   >  1:4140447/1‑101 (MQ=255)
   gcgcCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCcgcg                                                                                                   <  2:5726350/101‑1 (MQ=255)
    cgcCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCgg                                                                                                  <  1:2897564/101‑1 (MQ=255)
     gcCGTGGTCAGGGCCCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGt                                                                                                 <  2:2816677/101‑1 (MQ=255)
       cGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGttt                                                                                               <  2:1212876/101‑1 (MQ=255)
        gTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTc                                                                                              >  1:2051428/1‑101 (MQ=255)
         tGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTcc                                                                                             >  1:5613078/1‑101 (MQ=255)
         tGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTcc                                                                                             >  1:1149729/1‑101 (MQ=255)
          ggTCAGGGCGCGGATCTGCGCGGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            <  2:2653063/101‑1 (MQ=255)
          ggTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            <  1:2460654/101‑1 (MQ=255)
          ggTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            <  2:2710686/101‑1 (MQ=255)
          ggTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            >  2:5579898/1‑101 (MQ=255)
          ggTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCCCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            <  2:659296/101‑1 (MQ=255)
           gTCAGGGCGCGGTTGTGCGCCGTGTTCAGGCTTGACATCAGCGCCGTCCCAATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAg                                                                                           <  2:3860714/101‑1 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          >  1:3556872/1‑101 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          <  2:1269357/101‑1 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          >  2:753907/1‑101 (MQ=255)
              aGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgcgc                                                                                        >  1:124333/1‑101 (MQ=255)
              aGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgcgc                                                                                        >  2:669120/1‑101 (MQ=255)
               gggCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGcgcc                                                                                       >  1:308646/1‑101 (MQ=255)
                     ggATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATc                                                                                 >  2:3940836/1‑101 (MQ=255)
                      gATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCt                                                                                <  1:2652439/101‑1 (MQ=255)
                      gATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGAGATATCCGCGGCTTCCAGCGCCGCGATCt                                                                                >  2:2838922/1‑101 (MQ=255)
                            cgcCGTGCTCAGGCTTGTCATCAGCGCGTTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCg                                                                          <  2:4601466/101‑1 (MQ=255)
                            cgcCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCg                                                                          <  1:3919648/101‑1 (MQ=255)
                              ccGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCATCGCCGCGATCTGCGCCGTg                                                                        <  2:3150329/101‑1 (MQ=255)
                                gTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCt                                                                      >  2:3083011/1‑101 (MQ=255)
                                gTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCt                                                                      <  1:3971913/101‑1 (MQ=255)
                                  gCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                    <  1:1094182/101‑1 (MQ=255)
                                   cTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAg                                                                   >  2:3493918/1‑101 (MQ=255)
                                    tCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                  >  2:2092595/1‑101 (MQ=255)
                                     cAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                                                 >  2:1482960/1‑101 (MQ=255)
                                      aGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                                                >  2:4784605/1‑101 (MQ=255)
                                       ggCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                                                               <  1:183379/101‑1 (MQ=255)
                                        gCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                              <  1:1887758/101‑1 (MQ=255)
                                        gCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                              <  2:3385354/101‑1 (MQ=255)
                                        ctttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                              <  2:1039052/99‑1 (MQ=255)
                                          ttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgag                                                            >  1:1039127/1‑99 (MQ=255)
                                          ttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCcgcg                                                            >  1:3158048/1‑101 (MQ=255)
                                          ttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCCCAGCGCcgcg                                                            >  2:1228220/1‑101 (MQ=255)
                                           tGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGa                                                           >  1:3806016/1‑101 (MQ=255)
                                            ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                          >  1:3419878/1‑101 (MQ=255)
                                            ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                          >  2:3725690/1‑101 (MQ=255)
                                            ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                          <  1:2916218/101‑1 (MQ=255)
                                             gCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATc                                                         >  2:5291766/1‑101 (MQ=255)
                                              cATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCt                                                        <  1:5580190/101‑1 (MQ=255)
                                                   gcgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTc                                                   <  1:3339251/101‑1 (MQ=255)
                                                    cgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcg                                                  <  2:4413957/101‑1 (MQ=255)
                                                     gcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcgc                                                 >  2:3042148/1‑101 (MQ=255)
                                                       cGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCgcgc                                               >  1:5354050/1‑101 (MQ=255)
                                                       cGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCgcgc                                               >  2:2041730/1‑101 (MQ=255)
                                                        gTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCt                                              >  2:1766575/1‑101 (MQ=255)
                                                         tCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                             >  2:5556440/1‑101 (MQ=255)
                                                           ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                           <  1:753975/101‑1 (MQ=255)
                                                           ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                           <  1:2408117/101‑1 (MQ=255)
                                                           ccGACCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                           <  2:1149648/101‑1 (MQ=255)
                                                             gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                         >  1:5241026/1‑101 (MQ=255)
                                                             gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTACAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                         <  1:2839083/101‑1 (MQ=255)
                                                              aTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                        >  1:1601275/1‑101 (MQ=255)
                                                               tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCgg                                       >  2:4350879/1‑100 (MQ=255)
                                                                ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                      >  1:3706346/1‑101 (MQ=255)
                                                                ccccGCCATGGCCGACACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCTGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                      >  1:4883198/1‑101 (MQ=255)
                                                                   cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                   >  2:2412813/1‑101 (MQ=255)
                                                                    gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                  >  1:4395710/1‑101 (MQ=255)
                                                                      cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                >  2:4179909/1‑101 (MQ=255)
                                                                      cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                <  1:2092712/101‑1 (MQ=255)
                                                                        tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                              >  2:5216658/1‑101 (MQ=255)
                                                                         ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                             >  2:2715918/1‑101 (MQ=255)
                                                                          gCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcgc                            <  1:1463733/101‑1 (MQ=255)
                                                                            cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                          <  2:2550349/101‑1 (MQ=255)
                                                                             gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                         >  1:701755/1‑101 (MQ=255)
                                                                                aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGt                      >  2:3684590/1‑101 (MQ=255)
                                                                                aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGt                      >  1:3187563/1‑101 (MQ=255)
                                                                                   gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                   >  2:5773114/1‑101 (MQ=255)
                                                                                    aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                  <  2:5030534/101‑1 (MQ=255)
                                                                                     aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                 >  2:5405928/1‑101 (MQ=255)
                                                                                      aGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcgc                >  1:4156722/1‑101 (MQ=255)
                                                                                      aGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcgc                >  1:5380993/1‑101 (MQ=255)
                                                                                       gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc               >  2:514901/1‑101 (MQ=255)
                                                                                        cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa              >  2:3255479/1‑101 (MQ=255)
                                                                                        cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa              >  1:1711652/1‑101 (MQ=255)
                                                                                         tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcccc             >  1:1361434/1‑99 (MQ=255)
                                                                                         cgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc             >  1:2233743/9‑101 (MQ=2)
                                                                                          gcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg            >  1:1257668/8‑101 (MQ=2)
                                                                                           caccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa           >  1:3549038/7‑101 (MQ=2)
                                                                                              gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt        <  1:166916/101‑1 (MQ=255)
                                                                                              gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt        >  2:4342354/1‑101 (MQ=255)
                                                                                              gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt        <  2:3556655/101‑1 (MQ=255)
                                                                                              cagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt        >  2:2604188/4‑101 (MQ=2)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc       >  1:4004075/1‑101 (MQ=255)
                                                                                               agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGTTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc       <  1:4934867/99‑1 (MQ=2)
                                                                                               agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc       >  1:4472149/3‑101 (MQ=2)
                                                                                                tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa      >  2:4818464/1‑101 (MQ=255)
                                                                                                gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa      <  2:4447785/100‑1 (MQ=2)
                                                                                                gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa      <  1:2164820/100‑1 (MQ=2)
                                                                                                  tCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAtt    <  2:5339618/101‑1 (MQ=255)
                                                                                                   ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc   <  1:3042322/101‑1 (MQ=255)
                                                                                                    cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg  <  2:4030/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCG  >  NC_014034/678653‑678853

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG