Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,583 G→C 3.5% T675R (ACG→AGG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5830G→C 3.5% 10.8 0.0 ‑0.8225T675R (ACG→AGG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (105/112):  total (109/116)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.52e-01

Only 100 of 254 total aligned reads displayed.

GATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATC  >  NC_014034/681483‑681683
                                                                                                    |                                                                                                    
gATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                                                                                                      <  2:3078098/101‑1 (MQ=255)
gATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGACGCCGTGCCCAGCGCCGCCACCg                                                                                                      >  1:2950399/1‑101 (MQ=255)
gATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGACGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                                                                                                      >  1:5016056/1‑101 (MQ=255)
 aTCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGt                                                                                                     >  1:2712848/1‑101 (MQ=255)
  tCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCTTCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCCTc                                                                                                    >  1:4661227/1‑101 (MQ=255)
  tCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTc                                                                                                    <  2:2222082/101‑1 (MQ=255)
    tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc                                                                                                  >  1:5095646/1‑101 (MQ=255)
     gcgcCGAGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc                                                                                                 <  1:2499546/101‑1 (MQ=255)
     gcgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc                                                                                                 <  2:4448469/101‑1 (MQ=255)
     gcgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc                                                                                                 <  1:3451563/101‑1 (MQ=255)
      cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                                                                >  1:3244871/1‑101 (MQ=255)
         cGAGCCCAGGCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                                                             <  2:4572470/101‑1 (MQ=255)
         cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                                                             >  2:3984092/1‑101 (MQ=255)
          gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                                                            >  2:4061720/1‑101 (MQ=255)
           ggCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                                                           <  2:4379988/100‑1 (MQ=255)
           aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                                                           <  2:2098107/101‑1 (MQ=255)
               cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg                                                                                       >  1:1858703/1‑101 (MQ=255)
                aGACGCGCCGCCTGCGCCGTCGTCAGCTCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                                      <  2:416412/101‑1 (MQ=255)
                aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                                      <  1:3738961/101‑1 (MQ=255)
                  aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg                                                                                    >  2:2272809/1‑101 (MQ=255)
                   gccgccgcCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgc                                                                                   >  1:5604146/3‑101 (MQ=21)
                   cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGctccgc                                                                                   >  1:3800753/1‑101 (MQ=255)
                   cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccgc                                                                                   >  1:1325267/1‑101 (MQ=255)
                      gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                                                                >  2:4640462/1‑101 (MQ=255)
                      cccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaag                                                                                <  2:3429169/100‑4 (MQ=25)
                        cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTTCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaagct                                                                              <  2:4741745/101‑6 (MQ=37)
                        cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                                                              <  1:73095/101‑1 (MQ=255)
                         gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGTGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                                             >  1:5513761/1‑101 (MQ=255)
                         gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                                             >  2:5449905/1‑101 (MQ=255)
                         gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                                             >  1:1660194/1‑101 (MQ=255)
                          ccTGCGCCGGTTTAACCGCTACGATGGGCTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCCCCGCCCCCGTCGCCGAAGCCAGCGCCGCGATCTgc                                                                            <  2:315563/101‑1 (MQ=255)
                           cTGCGCCGTCGTCAGCGACACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                                                           <  2:5194018/101‑1 (MQ=255)
                            tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                                                          <  2:1668181/101‑1 (MQ=255)
                             gcgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGcc                                                                         <  2:493938/101‑1 (MQ=255)
                              cgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCg                                                                        <  2:4697058/101‑1 (MQ=255)
                               gcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGCTCTGTGCCGt                                                                       >  1:1909680/1‑101 (MQ=255)
                                    cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                  <  1:5393094/101‑1 (MQ=255)
                                    cgtcAGCGCCACGATGTCAACGGTGCCAAAGCCGCGGCTCGCCGCCGCGCCCAGCGTCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGCGCCCa                                                                  >  2:694141/1‑101 (MQ=38)
                                     gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGACAGCGCCGCGATCTGCGCCGTGCTCAg                                                                 >  1:1037921/1‑101 (MQ=255)
                                      tcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                <  2:5611005/101‑1 (MQ=255)
                                      tcAGCGCCACGATGTCATCGGTGCCAAAGCAGAGGATCGCCGCCGTGCCCAGCGCCGCCACCGTAGCCGAAGCAAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                >  1:3614679/1‑101 (MQ=38)
                                      gcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                <  1:4145674/100‑1 (MQ=255)
                                        aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                                              >  2:5512792/1‑101 (MQ=255)
                                           gcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                           >  1:3888848/1‑101 (MQ=255)
                                            ccACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                                                          >  2:1473902/1‑101 (MQ=255)
                                             cACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgccg                                                         >  2:3509928/1‑101 (MQ=255)
                                                gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                                      >  1:3758141/1‑101 (MQ=255)
                                                gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                                      <  2:1532151/101‑1 (MQ=255)
                                                gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                                      >  2:2681942/1‑101 (MQ=255)
                                                  tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                                                    <  1:3984336/101‑1 (MQ=255)
                                                  tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                                                    <  2:1999730/101‑1 (MQ=255)
                                                   gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                                                   >  1:1767462/1‑101 (MQ=255)
                                                    tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc                                                  >  2:1158706/1‑101 (MQ=255)
                                                       tCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGa                                               <  2:1762447/101‑1 (MQ=255)
                                                        cGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAg                                              >  1:3156509/1‑101 (MQ=255)
                                                          gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc                                            >  2:3010727/1‑101 (MQ=255)
                                                          gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc                                            <  1:5151300/101‑1 (MQ=255)
                                                            gCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCaa                                          <  2:4119137/101‑1 (MQ=255)
                                                            gCCAAAGCCGCGGACCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCaa                                          <  2:5015775/101‑1 (MQ=255)
                                                               aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                                       <  1:4847545/101‑1 (MQ=255)
                                                               aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                                       <  1:449100/101‑1 (MQ=255)
                                                               aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                                       <  1:3358453/101‑1 (MQ=255)
                                                               aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                                       <  1:2079385/101‑1 (MQ=255)
                                                                aaGCCGCGGATCGCCGACGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGccc                                      <  1:2225610/101‑1 (MQ=255)
                                                                 ggCCGGGGCTCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCg                                     <  1:1130725/100‑1 (MQ=255)
                                                                  gCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCgg                                    <  1:1456364/101‑1 (MQ=255)
                                                                   ccGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGc                                   <  1:4427114/101‑1 (MQ=255)
                                                                    ggcgCATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGcc                                  <  1:417787/100‑1 (MQ=255)
                                                                    cgcgGCTCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGcc                                  <  2:1264595/101‑1 (MQ=255)
                                                                       ggATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCAcc                               <  1:2565937/101‑1 (MQ=255)
                                                                        gATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCg                              <  1:2420971/101‑1 (MQ=255)
                                                                            gccgccGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAg                          >  1:2972397/1‑101 (MQ=255)
                                                                             tcgccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAgg                         <  2:1092557/100‑1 (MQ=255)
                                                                               gccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATATGCGCCGTGCTCATCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt                       >  2:1331937/1‑101 (MQ=255)
                                                                               cccgTGCCCAGCCCCCCCCCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt                       <  1:1086176/100‑1 (MQ=255)
                                                                                ccgTGCCCAGCGCCGCCACCGTCGCCGAGGCCAGCTCCGCGATCTCCGCCGTGCTCGGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTg                      <  2:3800511/101‑1 (MQ=255)
                                                                                 cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCGGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCAGGCCACCGCAAGGCTgg                     >  2:1865450/1‑101 (MQ=255)
                                                                                 cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTgg                     >  2:1557109/1‑101 (MQ=255)
                                                                                  gTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                    <  1:5291040/101‑1 (MQ=255)
                                                                                  gTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                    <  2:2954483/101‑1 (MQ=255)
                                                                                   tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                   <  2:1909571/101‑1 (MQ=255)
                                                                                   tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                   >  2:2654839/1‑101 (MQ=255)
                                                                                    gCCCAGCGCCGCCGCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                  <  2:4269005/101‑1 (MQ=255)
                                                                                    gCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                  <  1:2180059/101‑1 (MQ=255)
                                                                                    gCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                  <  2:3735683/101‑1 (MQ=255)
                                                                                       cAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcg               <  1:3032893/101‑1 (MQ=255)
                                                                                       cAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcg               >  2:1833954/1‑101 (MQ=255)
                                                                                            ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGATCAGCGCCGCAGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGAGCCg          >  1:911917/1‑101 (MQ=255)
                                                                                             cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGCGCGCGCCgc         >  2:1427792/1‑100 (MQ=255)
                                                                                             cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGa         <  1:1012544/101‑1 (MQ=255)
                                                                                              gccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc        >  2:240255/1‑101 (MQ=255)
                                                                                               ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc       <  1:695258/101‑1 (MQ=255)
                                                                                               ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc       <  1:3010901/101‑1 (MQ=255)
                                                                                               ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc       <  1:2624951/101‑1 (MQ=255)
                                                                                                ccccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt      <  2:2001814/99‑1 (MQ=255)
                                                                                                cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCCCAAGGCTGGAGAGCGCGCCGACCt      >  2:575274/1‑101 (MQ=255)
                                                                                                 cccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg     <  2:5095361/100‑1 (MQ=255)
                                                                                                 actgTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg     >  1:3294725/4‑101 (MQ=255)
                                                                                                 aCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg     >  2:3305142/1‑101 (MQ=255)
                                                                                                    gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc  <  1:3503754/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATC  >  NC_014034/681483‑681683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG