Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,590 A→T 3.5% S673T (TCG→ATG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5900A→T 3.5% 13.5 0.0 ‑0.1231S673T (TCG→ACG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (109/114):  total (113/118)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.61e-01

Only 100 of 253 total aligned reads displayed.

GCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTC  >  NC_014034/681490‑681690
                                                                                                    |                                                                                                    
gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                                                                      >  2:1076627/1‑101 (MQ=255)
gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                                                                      >  1:4697339/1‑101 (MQ=255)
  caggCCCAGCCCCGCCGCCTGCGCCGTCGTCAGCGCTACGATCACATCGGTTCAAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCCCCGTCGCCGAAg                                                                                                    <  2:3285039/98‑1 (MQ=21)
   gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                                                                   >  2:4063305/1‑101 (MQ=255)
    aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                                                                  <  2:2098107/101‑1 (MQ=255)
     gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa                                                                                                 >  2:1476378/1‑101 (MQ=255)
        cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg                                                                                              <  2:5260599/101‑1 (MQ=255)
         aGACGCGCCGCCTGCGCCGTCGTCAGCTCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                                             <  2:416412/101‑1 (MQ=255)
         aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                                             <  1:3738961/101‑1 (MQ=255)
         aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                                             >  1:5430226/1‑101 (MQ=255)
            cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGctccgc                                                                                          >  1:3800753/1‑101 (MQ=255)
              cgccgcCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaa                                                                                        >  2:5489187/1‑99 (MQ=25)
               gccgccTGCGCCGTCGTGAGCGCCACGATGTCATCGGTGCCAAAACCGCGGATCGCCGCCGTGCCCAGCGTCGCCACCGTCGCCGAAGACAGCGCCGCGAt                                                                                       >  2:4761188/1‑101 (MQ=38)
               cccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaag                                                                                       <  2:3429169/100‑4 (MQ=25)
                ccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                                                                      <  1:2066402/101‑1 (MQ=255)
                 cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                                                                     <  2:1240391/101‑1 (MQ=255)
                 cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                                                                     <  1:73095/101‑1 (MQ=255)
                  gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGTGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                                                    >  1:5513761/1‑101 (MQ=255)
                   ccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTgc                                                                                   >  1:1794787/1‑101 (MQ=37)
                   ccTGCGCCGGTTTAACCGCTACGATGGGCTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCCCCGCCCCCGTCGCCGAAGCCAGCGCCGCGATCTgc                                                                                   <  2:315563/101‑1 (MQ=255)
                    cTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                                                                  >  1:2954650/1‑101 (MQ=255)
                     tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                                                                 <  1:2588542/101‑1 (MQ=255)
                        gcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGCTCTGTGCCGt                                                                              >  1:1909680/1‑101 (MQ=255)
                         ccGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTg                                                                             <  2:4621477/101‑1 (MQ=255)
                              gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAg                                                                        <  2:4699843/101‑1 (MQ=255)
                              gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGACAGCGCCGCGATCTGCGCCGTGCTCAg                                                                        >  1:1037921/1‑101 (MQ=255)
                               tcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                       >  1:5224810/1‑101 (MQ=255)
                               tcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                       >  1:626087/1‑101 (MQ=255)
                               tcAGCGCCACGATGTCATCGGTGCCAAAGCAGAGGATCGCCGCCGTGCCCAGCGCCGCCACCGTAGCCGAAGCAAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                       >  1:3614679/1‑101 (MQ=38)
                               gcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                                                                       <  1:4145674/100‑1 (MQ=255)
                                cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                                                      <  2:2245766/101‑1 (MQ=255)
                                 ggcgtcgcGATGTCATCGGTGCCAAAGCCGCGGATCGGCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                                                     >  2:513858/8‑101 (MQ=255)
                                 aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                                                     <  1:5663288/101‑1 (MQ=255)
                                  gcgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                                                                    <  1:3185854/101‑1 (MQ=255)
                                   cgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                                   >  1:5045110/1‑101 (MQ=255)
                                    gcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                                  >  1:3888848/1‑101 (MQ=255)
                                     ctaCGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                                                                 <  2:5554222/99‑1 (MQ=255)
                                      cACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgccg                                                                <  1:4640738/101‑1 (MQ=255)
                                      cACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgccg                                                                >  2:3343895/1‑101 (MQ=255)
                                      cACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgccg                                                                >  2:3509928/1‑101 (MQ=255)
                                         gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                                             >  1:3758141/1‑101 (MQ=255)
                                         gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                                             >  2:4394697/1‑101 (MQ=255)
                                           tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                                                           <  1:3984336/101‑1 (MQ=255)
                                           tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                                                           >  1:2643561/1‑101 (MQ=255)
                                           tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                                                           <  2:1999730/101‑1 (MQ=255)
                                            gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                                                          >  1:1767462/1‑101 (MQ=255)
                                             tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc                                                         >  2:1602433/1‑101 (MQ=255)
                                             tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGCCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc                                                         >  1:3485063/1‑101 (MQ=255)
                                              cATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAcc                                                        <  1:2181166/101‑1 (MQ=255)
                                                tCGGTGCGAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGa                                                      <  2:1971558/101‑1 (MQ=255)
                                                  ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc                                                    >  2:5078470/1‑101 (MQ=255)
                                                   gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCGCCGAGcc                                                   <  1:1404808/101‑1 (MQ=255)
                                                   gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc                                                   >  2:3010727/1‑101 (MQ=255)
                                                   gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc                                                   >  2:1150129/1‑101 (MQ=255)
                                                   gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc                                                   <  2:1363539/101‑1 (MQ=255)
                                                     gCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCaa                                                 <  1:4446537/101‑1 (MQ=255)
                                                      ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg                                                >  2:695201/1‑101 (MQ=255)
                                                       cAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGc                                               >  1:1292932/1‑101 (MQ=255)
                                                        aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                                              <  1:3358453/101‑1 (MQ=255)
                                                         aaGCCGCGGATCGCCGACGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGccc                                             <  1:2225610/101‑1 (MQ=255)
                                                          ggCCGGGGCTCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCg                                            <  1:1130725/100‑1 (MQ=255)
                                                           gCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCAGCACCGAGCCAAGGCCgg                                           >  2:4048295/1‑101 (MQ=255)
                                                             cgcgGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGcc                                         >  2:154726/1‑101 (MQ=255)
                                                                 gATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCg                                     <  2:2707735/101‑1 (MQ=255)
                                                                   tCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCa                                   <  2:4939799/101‑1 (MQ=255)
                                                                    cgccgcCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCaa                                  >  2:3697644/1‑101 (MQ=255)
                                                                     gccgccGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAg                                 >  1:2972397/1‑101 (MQ=255)
                                                                        gccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATATGCGCCGTGCTCATCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt                              >  2:1331937/1‑101 (MQ=255)
                                                                        cccgTGCCCAGCCCCCCCCCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt                              <  1:1086176/100‑1 (MQ=255)
                                                                         ccgTGCCCAGCGCCGCCACCGTCGCCGAGGCCAGCTCCGCGATCTCCGCCGTGCTCGGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTg                             <  2:3800511/101‑1 (MQ=255)
                                                                          cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCGGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCAGGCCACCGCAAGGCTgg                            >  2:1865450/1‑101 (MQ=255)
                                                                          cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTgg                            <  1:1629065/101‑1 (MQ=255)
                                                                          cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTgg                            >  2:5791894/1‑101 (MQ=255)
                                                                           gtccccAGCGCCGCCCCCGTCGCCCAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                           <  2:1432885/98‑1 (MQ=255)
                                                                           gTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                           <  2:2954483/101‑1 (MQ=255)
                                                                            tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                          >  2:2654839/1‑101 (MQ=255)
                                                                            tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                          <  1:3014907/101‑1 (MQ=255)
                                                                             gCCCAGCGCCGCCGCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                         <  2:4269005/101‑1 (MQ=255)
                                                                             gCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                         >  2:5731128/1‑101 (MQ=255)
                                                                                cAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcg                      >  2:1687158/1‑101 (MQ=255)
                                                                                cAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcg                      >  2:1833954/1‑101 (MQ=255)
                                                                                 aGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcgc                     <  2:4648655/101‑1 (MQ=255)
                                                                                     ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                 >  1:315091/1‑101 (MQ=255)
                                                                                      cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGa                <  1:1012544/101‑1 (MQ=255)
                                                                                       gccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc               >  2:240255/1‑101 (MQ=255)
                                                                                        ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc              <  1:513908/101‑1 (MQ=255)
                                                                                          actgTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg            >  1:3294725/4‑101 (MQ=255)
                                                                                             gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc         >  1:4301268/1‑101 (MQ=255)
                                                                                             gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc         >  2:4520701/1‑101 (MQ=255)
                                                                                              tCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCg        >  2:2472921/1‑101 (MQ=255)
                                                                                              ccGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCg        <  2:1767363/100‑1 (MQ=255)
                                                                                               cGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCgg       >  1:5349754/1‑101 (MQ=255)
                                                                                               cGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCgg       >  1:2378587/1‑101 (MQ=255)
                                                                                                gCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGt      <  2:4141390/101‑1 (MQ=255)
                                                                                                  cGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc    >  1:1670163/1‑101 (MQ=255)
                                                                                                  cGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc    >  1:807077/1‑101 (MQ=255)
                                                                                                  cGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc    >  2:2060638/1‑101 (MQ=255)
                                                                                                   gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt   <  1:2535207/101‑1 (MQ=255)
                                                                                                    aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc  >  1:2435274/1‑101 (MQ=255)
                                                                                                    aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc  <  2:3893612/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTC  >  NC_014034/681490‑681690

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG