Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,707	3 bp→TCC	100%	intergenic (‑131/‑270)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,707	0	G→T	100.0%	150.5	58	intergenic (‑131/‑272)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/58):  ref base (0/0):  total (0/58)
*	NC_014034	1,732,708	0	G→C	100.0%	152.8	58	intergenic (‑132/‑271)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/58):  ref base (0/0):  total (0/58)
*	NC_014034	1,732,709	0	G→C	100.0%	153.0	58	intergenic (‑133/‑270)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/58):  ref base (0/0):  total (0/58)

TAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..GGGC..GGGCGGGGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGC  >  NC_014034/1732608‑1732779                                                                                                              |||                                                                       taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..Cc..cggg..gggggg                                                                         >  1:2256537/1‑101 (MQ=255)    ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..CGGG..GGGGggggg                                                                      >  1:3589708/1‑100 (MQ=255)     gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGC..CGG.GGGGcc                                                                     >  1:2756947/1‑101 (MQ=255)     gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..CGGG..GGGGgggggg                                                                     >  1:2411964/1‑99 (MQ=255)         cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..CGG...GGCCggggggcggcc                                                                >  2:5137377/1‑94 (MQ=255)          ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.ACCCC..GGGC..GGGGgggggcgcc                                                                  >  2:2160145/1‑96 (MQ=255)          ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGC..GGGGGGGGcccccc                                                                 >  2:1932832/1‑100 (MQ=255)              ggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCCCGGGGGGGGGGCGGGGG.G.C..CC..GGGG..GGGCgggggggggcgcc                                                              >  1:4914475/1‑92 (MQ=255)                 gagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..CGGG..GGGGGGGGCCCCCCCCcccccc                                                         >  2:3472166/1‑100 (MQ=255)                  agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGGCGCGGC.G.A..CCCGGGGG..GGGGGGGGCCCCCCCCccccc                                                          >  1:5336502/1‑101 (MQ=255)                   ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCAccccc                                                          <  1:5029265/101‑1 (MQ=25)                   ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCAccccc                                                          <  2:3796094/101‑1 (MQ=25)                   ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCAccccc                                                          <  2:5540195/101‑1 (MQ=25)                   ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCAccccc                                                          <  1:1622517/101‑1 (MQ=25)                   ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCAccccc                                                          <  2:5346912/101‑1 (MQ=25)                    gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCg                                                         <  1:848036/101‑1 (MQ=25)                    gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCg                                                         <  2:4850876/101‑1 (MQ=25)                    gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCg                                                         <  2:5143971/101‑1 (MQ=25)                     tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAGGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCgg                                                        <  1:4942276/101‑1 (MQ=25)                     tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCgg                                                        <  1:4552891/101‑1 (MQ=25)                      aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGc                                                       <  2:3406267/101‑1 (MQ=25)                        aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..CGGG..GGGGGGGCCCCGCCCCccccccccccccg                                                  >  2:3502122/1‑93 (MQ=255)                           ccGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTAtt                                                  <  2:2610916/101‑1 (MQ=25)                           ccGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTAtt                                                  <  2:5485969/101‑1 (MQ=25)                            cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTAttt                                                 <  1:1884046/101‑1 (MQ=25)                            cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTAttt                                                 <  1:5188937/101‑1 (MQ=25)                             gTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTg                                                <  1:824374/101‑1 (MQ=21)                                ggTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTgcgc                                             <  2:399398/101‑1 (MQ=21)                                 gTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGcc                                            <  2:1744332/101‑1 (MQ=21)                                  tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCa                                           <  2:5349549/101‑1 (MQ=21)                                    cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCaag                                         <  2:4421544/101‑1 (MQ=21)                                       ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                      <  2:5262807/101‑1 (MQ=18)                                        gCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAagaaga                                     <  1:1155130/101‑1 (MQ=18)                                          ggAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                   <  1:5302905/101‑1 (MQ=18)                                          ggAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                   <  1:84661/101‑1 (MQ=18)                                          ggAAAGCCGCGTGCCAAGCAGGCTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                   <  1:4141648/101‑1 (MQ=18)                                           gAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                  <  2:5347890/101‑1 (MQ=18)                                            aaaGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACa                                 <  2:4423281/101‑1 (MQ=18)                                             aaGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                <  1:2213836/101‑1 (MQ=18)                                              aGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAg                               <  2:2199034/101‑1 (MQ=18)                                              aGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAg                               <  2:3039614/101‑1 (MQ=18)                                               gCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGc                              <  2:3410919/101‑1 (MQ=18)                                                 cgcgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCgg                            <  1:978252/101‑1 (MQ=18)                                                 cgcgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCgg                            <  1:5137663/101‑1 (MQ=18)                                                  gcgTGCGAAGCAGGGTGTCG..CCGCGGCGGGGCCCCGGCGG.C..CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCggg                           <  1:3917827/101‑1 (MQ=17)                                                  gcgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCggg                           <  1:1541876/101‑1 (MQ=18)                                                   cgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                          <  2:4109848/101‑1 (MQ=18)                                                   cgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                          <  2:391310/101‑1 (MQ=18)                                                   cgTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                          <  2:3589491/101‑1 (MQ=18)                                                    gTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                         <  2:2089767/101‑1 (MQ=18)                                                     tGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                        <  1:2478254/101‑1 (MQ=18)                                                     tGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                        <  1:2018076/101‑1 (MQ=18)                                                      gCCAAGCAGTGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCa                       <  1:1828841/101‑1 (MQ=18)                                                       cttccgatcttttCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                      <  2:4332799/91‑1 (MQ=17)                                                       ccAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                      <  2:3570389/101‑1 (MQ=18)                                                       ccAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                      <  2:2756786/101‑1 (MQ=18)                                                         aaGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGc                    <  1:277599/101‑1 (MQ=18)                                                           gCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccc                  <  2:1867666/101‑1 (MQ=18)                                                           gCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccc                  <  2:4184079/101‑1 (MQ=18)                                                               ggTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt              <  2:3223585/101‑1 (MQ=18)                                                               ggTTTCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt              <  1:4235101/101‑1 (MQ=18)                                                                gTTTCG..CCGCGGGGGGGCCGCGGC.GCC..CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTg             <  2:1615486/101‑1 (MQ=18)                                                                  ttCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa           <  1:1662443/101‑1 (MQ=18)                                                                  ttCG..CCGCGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa           <  1:3923805/101‑1 (MQ=18)                                                                     g..ccgcGGCGGGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGcc        <  1:24039/101‑1 (MQ=18)                                                                           gggcggGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt    <  2:3251576/100‑1 (MQ=18)                                                                           cggcggGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt    <  1:41899/101‑1 (MQ=18)                                                                            gggggggCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg   <  1:3937703/98‑1 (MQ=18)                                                                             gcggGGCCGCGGC.G.AC.CC..GGGCGGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc  <  1:2160267/101‑1 (MQ=18)                                                                                                              |||                                                                       TAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCG..CCGCGGCGGGGCCGCGGC.G.A..CC..GGGC..GGGCGGGGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGC  >  NC_014034/1732608‑1732779 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG