Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,620,690 2 bp→CT 100% coding (1050‑1051/1188 nt) livJ → branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,620,6900T→C100.0% 1074.1 281P350P (CCT→CCC) livJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (140/141):  ref base (0/0):  total (140/141)
*NC_0140342,620,6910A→T100.0% 1118.1 281N351Y (AAC→TAC) livJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (140/141):  ref base (0/0):  total (140/141)

Only 100 of 285 total aligned reads displayed.

GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785
                                                                                               ||                                                                                              
gAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACAt                                                                                            >  1:1456787/1‑101 (MQ=255)
 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCTTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  2:576973/101‑1 (MQ=255)
  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          >  2:402722/1‑101 (MQ=255)
  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          <  1:4736271/101‑1 (MQ=255)
   tatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGaa                                                                                         >  1:2847090/1‑101 (MQ=255)
   tatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGaa                                                                                         <  2:4731241/101‑1 (MQ=255)
    atatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAg                                                                                        >  2:323439/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       >  2:4964404/1‑101 (MQ=255)
      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      >  2:4742552/1‑101 (MQ=255)
       tCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGc                                                                                     >  2:1703707/1‑101 (MQ=255)
        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCg                                                                                    >  2:2691401/1‑101 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  1:1604429/1‑101 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  1:5534430/101‑1 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  >  1:3970721/1‑101 (MQ=255)
               aCGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGaa                                                                             <  2:3934351/101‑1 (MQ=255)
               aCGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGaa                                                                             <  2:4624725/101‑1 (MQ=255)
                cGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCGGCGCCGAACCCCTACATGAAGGGCGATGCGAAg                                                                            >  1:5012405/1‑101 (MQ=255)
                cGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAg                                                                            >  2:2234352/1‑101 (MQ=255)
                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  2:1521069/1‑101 (MQ=255)
                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  1:2981926/1‑101 (MQ=255)
                  cTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCt                                                                          >  2:329119/1‑101 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         <  1:5736726/101‑1 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         <  1:4085732/101‑1 (MQ=255)
                     aaaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAg                                                                       >  1:2096337/1‑101 (MQ=255)
                      aaGCCGATCCGAAGGCCCTGACCGACAGTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      <  2:5366520/101‑1 (MQ=255)
                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  <  2:1128890/101‑1 (MQ=255)
                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  <  2:1504424/101‑1 (MQ=255)
                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  >  2:2903629/1‑101 (MQ=255)
                           gATCTGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCCCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 <  1:5396523/101‑1 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 >  2:4245349/1‑101 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 <  1:1344977/101‑1 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 >  1:1531762/1‑101 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                >  2:421039/1‑101 (MQ=255)
                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  1:2657922/101‑1 (MQ=255)
                              ccGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCgg                                                              >  2:4167480/1‑101 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  2:3715754/1‑101 (MQ=255)
                                  aGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacc                                                          <  1:3909878/101‑1 (MQ=255)
                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        <  2:1631649/101‑1 (MQ=255)
                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        >  2:359524/1‑101 (MQ=255)
                                     gCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCaccacc                                                       <  2:5412933/101‑1 (MQ=255)
                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     <  1:817082/101‑1 (MQ=255)
                                        tGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCg                                                    <  2:2494545/101‑1 (MQ=255)
                                         gACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGt                                                   <  1:2472911/101‑1 (MQ=255)
                                          aCCGACATTGCCGCCTTCCAGACGGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGGGCTATGTCGGCACCACGTCggt                                                  >  2:1535973/1‑99 (MQ=255)
                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGtt                                                  >  2:2948608/1‑101 (MQ=255)
                                           ccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTc                                                 <  1:3783818/101‑1 (MQ=255)
                                             gACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCgg                                               >  1:4618836/1‑101 (MQ=255)
                                              aCATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCggg                                              >  1:2497660/1‑101 (MQ=255)
                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            >  2:1574929/1‑101 (MQ=255)
                                                aTTGCCCCCTTCAAGCCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGTTATGTCGGCACCACCTCGTTCGGGCa                                            <  1:1829177/101‑1 (MQ=255)
                                                    ccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAg                                        <  2:638615/101‑1 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       >  2:1879843/1‑101 (MQ=255)
                                                      gccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCg                                      >  2:3440189/1‑101 (MQ=255)
                                                       ccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCgg                                     <  1:3311819/101‑1 (MQ=255)
                                                        cTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGc                                    <  2:5688047/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  1:3232108/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  2:5649416/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  1:2236144/101‑1 (MQ=255)
                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   <  1:3695475/101‑1 (MQ=255)
                                                          tCAAGGCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  <  1:169845/101‑1 (MQ=255)
                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  <  1:3336984/101‑1 (MQ=255)
                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                >  2:2720879/1‑101 (MQ=255)
                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                >  2:3368110/1‑101 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  1:5637369/101‑1 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  2:2096220/101‑1 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  1:683886/101‑1 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               >  1:2438728/1‑101 (MQ=255)
                                                              gACCGCGATCGACAGTTTCGCCGCGCCGACCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGt                              <  1:2052172/101‑1 (MQ=255)
                                                              gACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGt                              >  2:2181649/1‑101 (MQ=255)
                                                               aCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTc                             >  1:2641921/1‑101 (MQ=255)
                                                                 cgcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCgg                           >  1:5447270/1‑101 (MQ=255)
                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGACGGGCGATGCGAAGCTGAGCCATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGt                          <  1:4947453/101‑1 (MQ=255)
                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCgct                          <  2:786465/101‑3 (MQ=255)
                                                                   cgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTg                         <  2:5315388/101‑1 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  2:2717685/101‑1 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  2:5029336/101‑1 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  1:1031737/101‑1 (MQ=255)
                                                                      tCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCg                      <  2:3117546/101‑1 (MQ=255)
                                                                      tCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGAGGCAGCTGTCGGTGCCg                      >  1:5667050/1‑101 (MQ=255)
                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    >  2:4982152/1‑101 (MQ=255)
                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    >  1:3200348/1‑101 (MQ=255)
                                                                         aCAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTc                   >  2:9044/1‑101 (MQ=255)
                                                                         aCAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTc                   <  2:4456518/101‑1 (MQ=255)
                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgt                 <  2:5456449/101‑1 (MQ=255)
                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgt                 <  1:4679114/101‑1 (MQ=255)
                                                                            gTTTCGCGGCGACGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                >  2:1181670/1‑101 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  2:2329827/101‑1 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  1:3395183/101‑1 (MQ=255)
                                                                             tttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtgg               >  1:2392155/1‑101 (MQ=255)
                                                                                     cgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGaa       <  2:255233/101‑1 (MQ=255)
                                                                                      gccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAAt      <  1:3019945/101‑1 (MQ=255)
                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    >  1:5239833/1‑101 (MQ=255)
                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTCAACGAATAc    <  1:529608/101‑1 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  2:13752/1‑101 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  1:420081/1‑101 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   <  1:3562434/101‑1 (MQ=255)
                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  >  2:196306/1‑101 (MQ=255)
                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  <  1:5210821/101‑1 (MQ=255)
                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  <  2:742068/101‑1 (MQ=255)
                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  <  1:193389/101‑1 (MQ=255)
                                                                                               ||                                                                                              
GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG