Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,876	2 bp→GC	100%	intergenic (‑208/+84)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,876	0	C→G	100.0%	73.6	28	intergenic (‑208/+85)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (26/2):  ref base (0/0):  total (26/2)
*	NC_014034	2,747,877	0	T→C	100.0%	103.4	36	intergenic (‑209/+84)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (34/2):  ref base (0/0):  total (34/2)

TTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGCG  >  NC_014034/2747801‑2747971                                                                               ||                                                                                               ttGCGGCAGGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGC.CCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGcc                                                                          <  2:1423690/101‑1 (MQ=17)        aTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCC.GCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGGCTTTGGGCCCTCcggg                                                                   >  2:617932/1‑101 (MQ=18)                                       gcgcCCGG.CCCGGCGGGCGAGAGGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAgg                                    <  1:2443025/101‑1 (MQ=18)                                         gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                                  >  1:5324893/1‑101 (MQ=18)                                         gcccgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                                  >  1:453111/1‑101 (MQ=18)                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                               >  2:73971/1‑101 (MQ=18)                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                               >  2:614539/1‑101 (MQ=18)                                            cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                               >  2:4163181/1‑101 (MQ=18)                                             gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                              >  2:3932973/1‑101 (MQ=18)                                             gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCCTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTgg                              >  1:1287893/1‑101 (MQ=18)                                               gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                             >  2:4724490/6‑101 (MQ=18)                                                 ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                           >  2:4937026/4‑101 (MQ=21)                                                  cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                          >  2:5063034/3‑101 (MQ=21)                                                   ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                         >  1:4392729/2‑101 (MQ=21)                                                    gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg                        >  1:5080120/1‑101 (MQ=21)                                                     cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc                       >  1:4178537/1‑101 (MQ=21)                                                     cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc                       >  2:396339/1‑101 (MQ=21)                                                      gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                      >  1:5253705/4‑101 (MQ=21)                                                      gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                      >  2:4788869/4‑101 (MQ=21)                                                        gcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCaa                    >  2:2199393/2‑101 (MQ=21)                                                         cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg                   >  1:3461423/1‑101 (MQ=21)                                                         cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg                   >  2:250118/1‑101 (MQ=21)                                                          gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                  >  1:1244583/1‑101 (MQ=21)                                                            gaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc                >  1:3696560/1‑101 (MQ=21)                                                            gaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc                >  1:1510926/1‑101 (MQ=21)                                                             aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa               >  2:1113049/1‑101 (MQ=21)                                                             aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa               >  1:4410865/1‑101 (MQ=21)                                                              aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg              >  1:1887305/1‑101 (MQ=21)                                                                   ccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAt         >  1:3168254/1‑101 (MQ=21)                                                                    cgccgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATg        >  1:2297952/6‑101 (MQ=255)                                                                     gccgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATCGCCAAGGCGATCGCCTTGGCCAAGGTCAGCTCATGc       >  2:4378460/5‑101 (MQ=21)                                                                      ccgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGcc      >  2:2774662/4‑101 (MQ=255)                                                                       cgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCa     >  2:1412337/3‑101 (MQ=255)                                                                       cgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCa     >  2:3961407/3‑101 (MQ=255)                                                                        gggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAg    >  2:3728914/2‑101 (MQ=255)                                                                        gggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAg    >  1:1935209/2‑101 (MQ=255)                                                                          gccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAgcg  >  2:3815395/1‑101 (MQ=255)                                                                               ||                                                                                               TTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGCG  >  NC_014034/2747801‑2747971 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG