Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,414 +A 1.3% intergenic (‑193/‑765) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4142.→A 1.3% 4.4 0.0 ‑0.4238intergenic (‑193/‑765)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (106/129):  total (107/131)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.95e-01

Only 100 of 251 total aligned reads displayed.

ACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434314‑1434510
                                                                                                        |                                                                                                 
 cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 <  2:84287/101‑1 (MQ=255)
 cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 >  2:3224161/1‑101 (MQ=255)
 cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 >  2:2781995/1‑101 (MQ=255)
   ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTc                                                                                               <  2:1918464/101‑1 (MQ=255)
      tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCt                                                                                            <  1:1020382/101‑1 (MQ=255)
       gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                           >  2:11699/1‑101 (MQ=255)
        ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                          >  2:3553652/1‑101 (MQ=255)
        ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                          <  2:3425182/101‑1 (MQ=255)
         tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccc                                                                                         >  1:1197404/1‑101 (MQ=255)
          ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                        <  2:2492931/101‑1 (MQ=255)
           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccccc                                                                                       <  1:4039312/101‑1 (MQ=255)
            gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                      <  2:633792/101‑1 (MQ=255)
            gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                      <  2:3906648/101‑1 (MQ=255)
             ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                                                     >  2:3043831/1‑101 (MQ=255)
             ggCCGAACCCCTGATCCTGCTGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                                                     <  1:2974635/101‑1 (MQ=255)
               cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcgcg                                                                                   >  1:2041740/1‑101 (MQ=255)
                 gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggg                                                                                 >  2:1353979/1‑101 (MQ=255)
                  aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                                >  2:964452/1‑101 (MQ=255)
                    cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                              <  2:3249262/101‑1 (MQ=255)
                     acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                             >  1:231090/1‑101 (MQ=255)
                     acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                             >  1:3865503/1‑101 (MQ=255)
                      ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                            <  2:2065677/101‑1 (MQ=255)
                           tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:3930046/101‑1 (MQ=255)
                             ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:2943274/94‑1 (MQ=2)
                              tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:4221862/101‑1 (MQ=255)
                              tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:4172298/101‑1 (MQ=255)
                              gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:4103861/95‑1 (MQ=2)
                               ttatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:512343/96‑1 (MQ=2)
                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                                 >  2:2369183/1‑100 (MQ=255)
                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  2:44880/1‑101 (MQ=255)
                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 <  1:322593/101‑1 (MQ=255)
                                 atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:3139733/4‑101 (MQ=2)
                                  gggGCGCACCCCCCGCGGGGGCGTTGATCGNAAC..GTCTCCGCTCGGCGTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacga                                                                <  1:2693548/101‑3 (MQ=25)
                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                               <  2:387163/101‑1 (MQ=255)
                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                               >  1:3737963/1‑101 (MQ=255)
                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  >  2:3618542/2‑101 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                             <  2:2193750/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAACGGa                                                             >  1:3661185/1‑101 (MQ=255)
                                       gcgccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              <  1:648318/98‑5 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              >  1:2438902/1‑97 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           >  1:3845767/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                          >  1:2442518/1‑101 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                         <  2:2679892/100‑1 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           <  1:2122348/101‑8 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  2:2927319/1‑94 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                       >  1:1758618/1‑94 (MQ=37)
                                            ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                       <  2:386047/101‑8 (MQ=37)
                                            ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                      <  1:2369359/101‑1 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                        >  1:509635/1‑91 (MQ=0)
                                             cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                        <  1:3223744/101‑11 (MQ=0)
                                             cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  1:641641/101‑1 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  1:4223063/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    >  2:639453/1‑101 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                                                   <  2:3983981/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:3634195/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              <  1:2831197/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                                             >  1:2098742/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                                             <  2:2451897/101‑1 (MQ=255)
                                                       cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                           <  1:291094/101‑1 (MQ=255)
                                                           gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                                       >  1:533225/1‑101 (MQ=255)
                                                           gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                                       <  2:2032111/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  2:3083926/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:1081316/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  1:254849/1‑101 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:3543519/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  2:1551704/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  2:2116587/101‑1 (MQ=255)
                                                                aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  >  1:851907/1‑101 (MQ=255)
                                                                 aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  2:1646461/101‑1 (MQ=255)
                                                                  aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  1:3008264/101‑1 (MQ=255)
                                                                  aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCACGGGACAGGCTTTGCa                                >  2:517457/1‑101 (MQ=255)
                                                                   c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                               <  2:4096162/101‑1 (MQ=255)
                                                                      gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:3699427/1‑101 (MQ=255)
                                                                      gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:455765/1‑101 (MQ=255)
                                                                      gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:927974/1‑101 (MQ=255)
                                                                      gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:3063356/1‑101 (MQ=255)
                                                                       tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGGGCCGAGGGACAGGCTTTGCAGTc                             >  1:470080/1‑101 (MQ=255)
                                                                       tctcCGCTCTGCTGCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                             <  2:1720205/101‑1 (MQ=255)
                                                                            gCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                        >  1:1279995/1‑101 (MQ=255)
                                                                            gCTCTGCTTCACG.CCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                        <  1:3634448/101‑1 (MQ=255)
                                                                                tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  1:169340/101‑1 (MQ=255)
                                                                                 gCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  1:2738112/101‑1 (MQ=255)
                                                                                   ttCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 <  2:1065881/101‑1 (MQ=255)
                                                                                   ttCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  2:829229/1‑101 (MQ=255)
                                                                                   ttCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  1:2266420/1‑101 (MQ=255)
                                                                                      aCG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggg              <  2:2396352/101‑1 (MQ=255)
                                                                                        g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            <  2:3719717/101‑1 (MQ=40)
                                                                                        g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            <  1:1340769/101‑1 (MQ=40)
                                                                                          gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg           >  2:1323642/1‑101 (MQ=40)
                                                                                            tGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCTTg         <  1:2414160/101‑1 (MQ=38)
                                                                                             gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  2:3821093/101‑1 (MQ=37)
                                                                                             gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  1:333244/101‑1 (MQ=37)
                                                                                                tCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     >  2:3520258/1‑101 (MQ=33)
                                                                                                 cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:2639938/101‑1 (MQ=32)
                                                                                                  aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:1939007/1‑101 (MQ=31)
                                                                                                  aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  2:3361797/101‑1 (MQ=31)
                                                                                                  aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:814558/101‑1 (MQ=31)
                                                                                                  aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:175288/1‑101 (MQ=31)
                                                                                                   aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:1430748/101‑1 (MQ=11)
                                                                                                   aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:1172537/101‑1 (MQ=11)
                                                                                                        |                                                                                                 
ACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434314‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG