Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,640,005 +GGCA 100% intergenic (+85/‑60) mccB → / → mccA methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,640,0051.→G100.0% 12.1 68intergenic (+85/‑60)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (60/1):  ref base (1/5):  total (62/6)
Fisher's exact test strand distribution p-value = 3.68e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.58e-03
*NC_0140341,640,0052.→G100.0% 12.2 67intergenic (+85/‑60)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (59/1):  ref base (1/5):  total (61/6)
Fisher's exact test strand distribution p-value = 3.97e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.44e-02
*NC_0140341,640,0053.→C100.0% 15.3 67intergenic (+85/‑60)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (59/1):  ref base (2/5):  total (61/6)
Fisher's exact test strand distribution p-value = 1.27e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.57e-02
*NC_0140341,640,0054.→A100.0% 15.5 68intergenic (+85/‑60)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (60/0):  ref base (2/5):  total (62/6)
Fisher's exact test strand distribution p-value = 2.17e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.37e-03

Only 100 of 104 total aligned reads displayed.

GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGC  >  NC_014034/1639905‑1640087
                                                                                                               ||||                                                                                               
taTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg                                                                                                     <  2:2749422/100‑1 (MQ=255)
 aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTAATT.TGGGG.GAGATGAAA.ACCATCCCTTT.CATC.CTGCCCCAAATAT.ACCCGGGGGCC..C..ggcg.....g                                                                                               >  2:723589/1‑101 (MQ=255)
 aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGAAATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....g                                                                                               >  1:2749641/1‑101 (MQ=255)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.CCCATCCCCTT.CTCC.TTTCCCCCAAAAT.CCCCGGGGGCC..C..gggg.....gg                                                                                              >  1:2008801/1‑100 (MQ=255)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAAATGAAA.ACCATCCCCTT.CATC.TTTCCCCAAATAT.CCCCGGGGGCC..C..cggg.....gg                                                                                              >  1:675195/1‑100 (MQ=37)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....gg                                                                                              <  1:2351668/101‑2 (MQ=255)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....gg                                                                                              <  1:2427775/101‑2 (MQ=255)
   cTTCCGGGTGGGACTTGCGATGCCTCCGGGGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggc                                                                                             <  1:3595389/101‑3 (MQ=25)
   cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGAGATTT.TGGGG.AAAATGAAA.CCCCTCCCTTT.CATC.TTTCCCCAAATAT.CCCCGGGGGCC..C..cggg.....ggg                                                                                             >  1:1156766/1‑99 (MQ=37)
    ttCCGGATTTGCCTTTCGATGCCGCGGGGGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca                                                                                            <  2:3579021/101‑4 (MQ=21)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGGAATTT.TGGGG.AAGATGAAA.ACCCTCCCTTT.TATC.TTTCCCCCAATAT.CCCCGGGGGCC..C..gggg.....gggg                                                                                            >  1:1854123/1‑98 (MQ=25)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca                                                                                            <  1:1567082/101‑4 (MQ=255)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca                                                                                            <  2:4235210/101‑4 (MQ=255)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca                                                                                            <  1:1261497/101‑4 (MQ=255)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GAGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggca                                                                                            <  1:3165721/101‑4 (MQ=255)
     tCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcag                                                                                           <  1:1953103/101‑5 (MQ=255)
       cGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGAGGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcagcg                                                                                         <  1:3642807/101‑7 (MQ=255)
        ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.ACCATCCCTTT.TTTC.TTTCCCCCAATATACCCGGGGGGCC..G..gggg.....ggggggg                                                                                         >  2:3588770/1‑95 (MQ=19)
        ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGC.AAGAAGGAA.CCCACCCCCTT.CATC.CTGCCCCAAATAT.CCCGGGGGGCC..C..gggg.....ggcggcgc                                                                                        >  1:525002/1‑94 (MQ=25)
         gATGTGACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGATGAAA.ACCATCCCTTT.CATC.TTTCCCCCAAAAT.CCCCGGGGGCC..C..gggg.....ggcagggcc                                                                                       >  1:2640565/1‑93 (MQ=25)
         gATGTGACTTTCGATGCCTCCGGCGGGGAGATTT.TGGGG.AAGATTAAA.CCCATCCCCCT.CGTC.TTTCCCCCAACAT.ACCTGGGCGCC..C..gggg.....ggcggcgtc                                                                                       >  2:317627/1‑93 (MQ=255)
           tgtgACTTTCGATGCCTCCGGCGGGGGTATTT.TGGGG.AAGAAGAAA.ACCATCCCCTT.TATCTTTCCCCCAAAAAT.CCCGGGGGGCC..C..gggg.....ggagcggccc                                                                                      >  2:3572823/1‑92 (MQ=255)
           tgtgACTTTCGATGCCTCCGGCGGGGGTATTT.GGGGG.CAGATAGAA.CACATCCCTTT.TCTC.TTGCCCCCAACATCCCCGGGGGGCC..G..GGGG.....G.....CG.....g..cgcccc                                                                          >  2:2288896/1‑100 (MQ=255)
                      aTGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCCTCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....GGGGN.CG.....C..CCCCCGCCACTTcc                                                                  >  2:509320/1‑101 (MQ=21)
                          cTCGGGCGGGTTTTTTG.GGGCC.AAGAT.AAA.CCCACCCCTTT.TCTT.CTGCCCCAAATTC.CCCGGGGGGGCGGG..GGGG.....G.....CGGCC..C..CCCCCGCCACTTCCGCCa                                                              <  2:1802052/101‑1 (MQ=255)
                           ccggggggggGTATTTGGGGGA.AAGAAAAAC.CCCCCCCCTTT.CACC.CTGCCCCAAATTC.CCCGGGGGGCC..G..GGGG.....GGC...CGCCC..C..CCCCCGCCACTTCCGcc                                                               <  2:1296645/95‑1 (MQ=255)
                              ggcggGGATATTT.TGGGC.AAGATGAAA.ACCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..CCGGGGGCA...G.....CG.....C..CCCCCCCCCCTTCCGCCACggg                                                          >  2:1750600/1‑101 (MQ=18)
                                cggGGATATTT.GGGGC.AAGATGAAA.CCGATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGc                                                        >  2:1271544/1‑101 (MQ=21)
                                 ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGNA.G.....CN.....C..CCCCCGCCACTTCCGCCACGGGGCa                                                       >  1:4222481/1‑101 (MQ=21)
                                 ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCa                                                       >  1:4156993/1‑101 (MQ=25)
                                 ggggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCa                                                       >  1:1193964/1‑101 (MQ=25)
                                  gggaatttt.GGGGA.AAAAATAAACCCCACCCTTTT.CATC.TTCCCCAAAATA..CCCGGGGGGCC..C..GGGG.....GGC...CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCa                                                       <  2:2504854/95‑1 (MQ=2)
                                   ggATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAgc                                                     >  1:2318810/1‑101 (MQ=21)
                                    gATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCAccgatctgaa                                                    >  2:2711613/1‑92 (MQ=18)
                                    gATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAgcg                                                    >  2:1760374/1‑101 (MQ=25)
                                     atatTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCAGCCACGGGGCAgcgc                                                   >  2:818114/1‑101 (MQ=21)
                                     atatTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....GGC...CG.....CGACCCCCGCCACTTCCGCCACGGGGCAgcgc                                                   >  2:1038688/1‑101 (MQ=21)
                                          tgggggC.AAGA.AAAA.CCCCCCCCTTT.CCCC.CTCCCCCAAAATC.CCCGGGGGGCC..C..GGGG.....GGC...CGGCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                               <  2:3862991/99‑1 (MQ=255)
                                          tgggggC.AAAATG.AA.CCCCCCCCCTT.CCTC.TTTCCCCAAAATC.CCCGGGGGGCC..C..GGGGGGCCAG.....CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                               <  1:3482028/99‑1 (MQ=255)
                                          t.GGGGCAAAGATAAAC.CCCCCCCCTTT.CTTC.TTCCCCAAAATAT.CCGGGGGGCCC..G..GGGG.....GCC...CGCC...C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                               <  2:2856948/101‑1 (MQ=9)
                                          t.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                              >  1:729171/1‑101 (MQ=25)
                                          t.GGGGC.AAAGAGAAA.CCCCCCCCTTTCCCTC.TTCCCCCAAATA..CCCGGGGGGCC..C..GGGGGGGCAG.....CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                               <  2:1223608/101‑1 (MQ=255)
                                           tgggcgaaaGATAAAC.CCCACCCCTTT.CATC.TCGCCCAAAATA..CCCGGGGGGCC..CGGGGGG.....G.....CGGCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                               <  2:1207293/94‑1 (MQ=12)
                                           gggggA.AAGATAAAC.CCCACCCTTTT.CATC.TGCCCCAAAAGAC.CGGGGGGGGCG..G..GGGG.....GGCCC.CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                              <  2:3308683/100‑1 (MQ=255)
                                            ggggC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGc                                             >  1:979752/1‑101 (MQ=25)
                                            ggggC.AAAAAAAAA.CCCCCCCCTTT.TCTC.CTGCCCCAAAATC.CCCGGGGGGCC..CGGGGGG.....G.....CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                              <  1:2500847/101‑1 (MQ=255)
                                                ggaaaaagAAC.CCCCTCCCTTT.TCTT.TTCCCCCAAAATT.CCGGGGGGGCC..C..GGGG.....GGGCCCCC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCg                                           <  1:3154015/94‑1 (MQ=255)
                                                c.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                         >  1:3714772/1‑101 (MQ=21)
                                                c.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                         >  1:3197070/1‑101 (MQ=25)
                                                  aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGTCAGCGCCCCGGCCGTTg                                        >  2:3801608/1‑101 (MQ=21)
                                                  aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                        >  2:3148872/1‑101 (MQ=25)
                                                  aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                        >  2:476050/1‑101 (MQ=25)
                                                  aaGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                        >  1:3512785/1‑101 (MQ=25)
                                                   aGATGAAA.CCCATCCCTTT.CATC.TTTCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                       >  1:696071/1‑101 (MQ=21)
                                                    gATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGAc                                      >  2:2111578/1‑101 (MQ=21)
                                                     aTGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                     >  1:2055857/1‑101 (MQ=21)
                                                      tGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa                                    >  1:229503/1‑101 (MQ=25)
                                                      tGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGa                                    >  1:1327950/1‑101 (MQ=25)
                                                      agcaa.CCCCTCCCTTT.CCCT.TTCCCCCAAAAGA.CCCGGGGGGCCGGG..GGGG.....G.....CGCCC..C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                     <  1:2520481/98‑1 (MQ=255)
                                                       gAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                   >  2:105336/1‑101 (MQ=25)
                                                       gAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                   >  1:2494886/1‑101 (MQ=25)
                                                         aa.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg                                 >  1:96620/1‑101 (MQ=25)
                                                          a.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGga                                >  2:3396684/1‑101 (MQ=25)
                                                            cccATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgag                               >  1:4188376/1‑101 (MQ=25)
                                                              cATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAc                             >  2:1430903/1‑101 (MQ=25)
                                                               cccctttt.CCTC.TTCCCCCAAATCC.CCGGGGGGGCC..G..GGGG.....GCCCC.CC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc                            <  1:1254920/96‑1 (MQ=255)
                                                               aTCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc                            >  1:1408632/1‑101 (MQ=25)
                                                                tCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg                           >  1:1624093/1‑101 (MQ=25)
                                                                tCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg                           >  1:2684234/1‑101 (MQ=25)
                                                                ccccTTT.CCCC.CTCCCCAAAATCT.CCCGGGGGGCC..C..GGGG.....GGGCCCCC.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc                            <  1:218398/100‑1 (MQ=255)
                                                                  ccTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcgcg                         >  2:4164487/1‑101 (MQ=25)
                                                                   cTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa                        >  1:2484647/1‑101 (MQ=25)
                                                                    ttt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt                       >  1:2824194/1‑101 (MQ=25)
                                                                     tt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                      >  2:3563178/1‑101 (MQ=25)
                                                                     tt.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                      >  1:1091080/1‑101 (MQ=25)
                                                                      t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                     >  1:15707/1‑101 (MQ=25)
                                                                      t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                     >  1:1838639/1‑101 (MQ=25)
                                                                      t.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                     >  1:2241139/1‑101 (MQ=25)
                                                                        cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATggt                    >  1:980072/1‑99 (MQ=25)
                                                                        cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                    >  1:204490/1‑101 (MQ=25)
                                                                        cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                    >  2:1963307/1‑101 (MQ=25)
                                                                        cATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                    >  2:1814031/1‑101 (MQ=25)
                                                                         tccccccccccAAAATT.CCGGGGGGGCC..C..GGGG.....GGCC..CGCC...C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                     <  1:2660119/94‑1 (MQ=255)
                                                                         aTC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt                   >  1:3487541/1‑101 (MQ=25)
                                                                          tC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc                  >  2:4220085/1‑101 (MQ=25)
                                                                          tC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc                  >  2:3738016/1‑101 (MQ=25)
                                                                           ctcccccccAAATCC.CCGGGGGGGCC..C..GGGG.....G.....GGCCCCCC..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt                   <  1:2248123/96‑1 (MQ=255)
                                                                           c.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCTCCGGCCGTTGACGAAGGAGACCGCGATGTTTCa                 >  1:3643864/1‑101 (MQ=25)
                                                                           c.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGAGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCa                 >  2:1398167/1‑101 (MQ=25)
                                                                              tGCCCCAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGCAGACCGCGATGTTTCaaa               >  1:4015837/1‑101 (MQ=25)
                                                                                ccccAAATAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAg             >  2:1861649/1‑101 (MQ=25)
                                                                                    aaaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcc         >  2:3708778/1‑101 (MQ=25)
                                                                                    aaaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcc         >  1:939176/1‑101 (MQ=25)
                                                                                     aaTAT.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt        >  1:2303192/1‑101 (MQ=25)
                                                                                      atat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg       >  2:47642/1‑101 (MQ=37)
                                                                                      atat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg       >  1:2178667/1‑101 (MQ=37)
                                                                                       tat.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGTCGAAGGAGACCGCGATGTTTCAAAAGATCCTGa      >  1:3677459/1‑101 (MQ=25)
                                                                                        at.CCCGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt     >  1:4056427/1‑101 (MQ=37)
                                                                                         t.CCCGGGGGGCC..C..GGGG.....GGCCC.CG.....C..CCCCCCCCCCTTCCGCCGCGGGGCAACGCCCCCGCCGGTGTCGAAGCGGAGGCCGACGGTTCAAAAGATCCTGATc    >  2:3688719/1‑101 (MQ=255)
                                                                                           nccGGGGGGCC..C..GGGGGGCA.G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCNGATc  >  1:963020/2‑100 (MQ=37)
                                                                                                               ||||                                                                                               
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTT.GGGGC.AAGATGAAA.CCCATCCCTTT.CATC.TTGCCCCAAATAT.CCCGGGGGGCC..C..GGGG.....G.....CG.....C..CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGC  >  NC_014034/1639905‑1640087

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG