BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,997,981	+G	96.3%	intergenic (‑46/‑119)	RCAP_rcc01844 ← / → RCAP_rcc01845	sensor histidine kinase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,997,981	1	.→G	96.3%	19.9 ‑0.7 ‑1.8	246	intergenic (‑46/‑119)	RCAP_rcc01844/RCAP_rcc01845	sensor histidine kinase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (110/127): ref base (3/6): total (113/133)
Fisher's exact test strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.57e-02

Only 100 of 258 total aligned reads displayed.


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076


 cAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g                                                                                                >  2:1113166/1‑101 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGCCACCCTGACATATCCAATGCTTAACCCGAGCAAGCTTC.gg                                                                                               >  1:1998430/1‑101 (MQ=255)


   gAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.ggg                                                                                              <  2:3370675/101‑1 (MQ=255)


    aaTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gggg                                                                                             >  1:3048505/1‑100 (MQ=255)


     aTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gggga                                                                                            >  1:1046609/1‑99 (MQ=255)


      tCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATACCCAATGCTTGACCCGAGCAAGCTTC.GGGgaa                                                                                           <  1:3728452/101‑1 (MQ=255)


       cGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaaa                                                                                          >  2:8809/1‑100 (MQ=255)


       cGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaaa                                                                                          <  1:486842/101‑2 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACg                                                                                         <  1:2246111/101‑1 (MQ=255)


           aaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGAt                                                                                       >  2:3712302/1‑101 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAc                                                                                     <  1:2061912/101‑1 (MQ=255)


               gTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAccc                                                                                   <  1:2724902/101‑1 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                  >  1:3439854/1‑101 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                  <  1:1547625/101‑1 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                  <  1:112082/101‑1 (MQ=255)


                  aCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAg                                                                                <  2:3989265/101‑1 (MQ=255)


                  aCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAg                                                                                <  2:4047667/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTTTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                               <  1:1605839/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                               <  2:1047355/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                               >  1:1697230/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGtt                                                                              >  1:1481058/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                           >  1:4085625/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                           <  2:4215119/101‑1 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCaa                                                                           <  1:3171067/101‑1 (MQ=255)


                         ggCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAgg                                                                         >  1:358509/1‑101 (MQ=255)


                          gCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGc                                                                        >  2:434986/1‑101 (MQ=255)


                          gCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGc                                                                        <  2:2731054/101‑1 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATAGCCAATGCTTGGCTCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGACg                                                                      >  2:696990/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                    <  2:1293459/101‑1 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                    >  1:2474624/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                    >  1:3910375/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                    >  1:2971063/1‑101 (MQ=255)


                                gagaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATg                                                                  <  1:131573/101‑1 (MQ=255)


                                 agaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATGCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATga                                                                 >  1:1001633/1‑100 (MQ=255)


                                  gaTTGTTGACGAATCCGCCATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCa                                                                <  1:1858535/101‑1 (MQ=255)


                                  gaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCa                                                                >  2:1488393/1‑101 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               >  2:480700/1‑101 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                              <  1:3784620/101‑1 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                              >  2:1139205/1‑101 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                              <  1:639059/101‑1 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                             <  2:303439/101‑1 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            >  2:1617724/1‑101 (MQ=255)


                                       ttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGc                                                           >  2:233473/1‑101 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          >  2:4157013/1‑101 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        <  1:136771/101‑1 (MQ=255)


                                             aaTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAgggg                                                     <  2:3186356/101‑1 (MQ=255)


                                                ccGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGc                                                  <  1:2658088/101‑1 (MQ=255)


                                                 cGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCt                                                 <  1:1355869/101‑1 (MQ=255)


                                                 cGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCt                                                 <  2:3439602/101‑1 (MQ=255)


                                                 cGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCt                                                 <  2:2783078/101‑1 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCACATGCCAGGGCGGCTa                                                >  2:3023235/1‑101 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                <  2:2150545/101‑1 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                <  1:233511/101‑1 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCGGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                              >  1:2801217/1‑101 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                              <  2:2616135/101‑1 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                             <  2:3892889/101‑1 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                             <  2:3062231/101‑1 (MQ=255)


                                                      tttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCt                                            >  1:1517183/1‑101 (MQ=255)


                                                       ttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                           >  1:2601940/1‑101 (MQ=255)


                                                        tttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCttt                                          <  1:983706/101‑1 (MQ=255)


                                                        tttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCttt                                          <  2:358458/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         >  1:423496/1‑101 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         <  2:3145880/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         >  2:231542/1‑101 (MQ=255)


                                                          tCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTg                                        <  1:3382120/101‑1 (MQ=255)


                                                           cGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCTAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGc                                       >  2:1750149/1‑101 (MQ=255)


                                                             gTCACCTTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTg                                     >  2:1834054/1‑101 (MQ=255)


                                                             gTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTg                                     >  2:3267371/1‑101 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   <  1:775442/101‑1 (MQ=255)


                                                                aCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAt                                  >  1:2880010/1‑101 (MQ=255)


                                                                aCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAt                                  <  1:2020400/101‑1 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                <  1:523027/101‑1 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                <  1:901823/101‑1 (MQ=255)


                                                                    tGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcgc                              <  1:3209750/101‑1 (MQ=255)


                                                                     gACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCGAAAGCCACGGCGGCTAACGTTTTGCTGAATTcgcg                             >  2:3790057/1‑101 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            <  1:3238643/101‑1 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            >  2:4205389/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          <  2:644523/101‑1 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  1:2836345/1‑101 (MQ=255)


                                                                         tatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtg                         <  2:2409154/101‑1 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                       <  2:195539/101‑1 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                       <  2:1517079/101‑1 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     <  2:595778/101‑1 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     >  2:768594/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     >  2:2421852/1‑101 (MQ=255)


                                                                              aaTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgtt                    <  1:3975731/101‑1 (MQ=255)


                                                                                tGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttgt                  >  2:3228979/1‑101 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                 >  2:1601598/1‑101 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                 >  1:2737038/1‑101 (MQ=255)


                                                                                   ttGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAt               >  2:3521746/1‑101 (MQ=255)


                                                                                   ttGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAt               <  2:3353412/101‑1 (MQ=255)


                                                                                   gtGACCCGAGCACGCTTCGGGGAAACGATACCAAAGTTCAAGGCCGCATACAAAAGCCAGGGGGGGTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAt               <  1:1660162/100‑1 (MQ=255)


                                                                                    tGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATg              >  2:1335849/1‑101 (MQ=255)


                                                                                      aCCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAg            <  1:3682680/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  2:2856809/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:3521999/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:3091592/101‑1 (MQ=255)


                                                                                          gAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGAt        >  1:692783/1‑101 (MQ=255)


                                                                                           aGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATg       >  1:1814094/1‑101 (MQ=255)


                                                                                             cAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATggg     >  1:2159016/1‑101 (MQ=255)


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG