BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,047,090	+C	85.4%	coding (20/879 nt)	potH1 ←	polyamine ABC transporter permease PotH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,047,090	1	.→C	85.4%	82.6 ‑0.4 ‑1.2	178	coding (20/879 nt)	potH1	polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand): new base (67/85): ref base (13/13): total (80/98)
Fisher's exact test strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.23e-02

Only 100 of 191 total aligned reads displayed.


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                               >  1:1986690/1‑101 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                              >  1:1302244/1‑100 (MQ=255)


 aacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                              >  1:4046934/2‑100 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgg                                                                                            >  2:3433018/1‑101 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgg                                                                                            >  2:13551/1‑101 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           >  2:486211/1‑100 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           >  1:4034375/1‑100 (MQ=255)


     aTGGCCCCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggt                                                                                          <  2:3050501/101‑3 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggtt                                                                                         >  1:2902175/1‑98 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                        <  2:3510749/101‑5 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATAAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                        <  2:3084347/101‑5 (MQ=255)


        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgg                                                                                       >  2:2178128/1‑96 (MQ=255)


        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgg                                                                                       <  2:2906459/101‑6 (MQ=255)


        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgg                                                                                       <  2:399856/101‑6 (MQ=255)


         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGATCGCGCCCACCG.Ccgggttgga                                                                                      >  1:1609688/1‑95 (MQ=255)


         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgga                                                                                      <  1:3962025/101‑7 (MQ=255)


          ctccAGCGTGCCCGCCAGAAACAAACCGGTAAAGAGCGGCGTTGTGCTGCCCATGAACCATCTTCGGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     <  2:643524/99‑8 (MQ=255)


           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggaga                                                                                    <  1:3449568/101‑9 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                   >  2:2769242/1‑101 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                  <  1:686370/101‑1 (MQ=255)


               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGcc                                                                                 <  2:450385/101‑1 (MQ=255)


               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGcc                                                                                 <  1:970076/101‑1 (MQ=255)


                cGGGCCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                <  1:1484615/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGAc                                                                              >  2:3876499/1‑101 (MQ=255)


                      cgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAc                                                                          <  1:2686579/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                        >  1:3789009/1‑101 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                        <  2:3738917/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                        <  1:600047/101‑1 (MQ=255)


                        caaacAAAAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                        <  1:52895/97‑1 (MQ=255)


                         cAGCAACAGAATTGCATGGATGAGGGTTGGGCTGACCAAGAACCAGCCGCTGGCAGCCTCCGAGCGCGCCCGCCGCCGGGTTGGAGATGCCGACATActct                                                                       <  2:285335/101‑1 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       >  1:2843766/1‑101 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       >  1:2411735/1‑101 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     >  1:3296611/1‑101 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     <  2:2461144/101‑1 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     >  1:2554294/1‑101 (MQ=255)


                             aaCGGAACTCCATAGATCAGCGTTAGGCTAACGATGACCCAGCCGCTCGCCGCCCCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                   <  2:338896/101‑1 (MQ=255)


                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                   >  2:2123723/1‑101 (MQ=255)


                              aCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct                                                                  >  2:2967428/1‑101 (MQ=255)


                               cAGACCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct.c                                                                <  1:2592560/101‑1 (MQ=255)


                               cAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct.c                                                                <  1:2959043/101‑1 (MQ=255)


                               cAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct.c                                                                >  1:1104757/1‑101 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               <  1:1725564/101‑1 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               <  2:2242626/101‑1 (MQ=255)


                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             >  1:3125598/1‑99 (MQ=255)


                                  aaCTGCATAGATCAGCCTTGGGCCGACCAAGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             <  2:2551186/101‑3 (MQ=255)


                                  aaCGGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             <  1:223460/101‑3 (MQ=255)


                                    ctgctcaGATCAGCTTTGGGTTGCCCTTGAACCGGCCTCTGGCCGCCTCCGAGCGCGCCCCCCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  1:4139080/95‑5 (MQ=255)


                                    cTGCATGTATCAGCGTTGGCCTGACAAGGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  2:1888399/101‑5 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  1:2067009/101‑5 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          >  2:2299687/1‑101 (MQ=255)


                                       cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg                                                         >  1:2044974/1‑101 (MQ=255)


                                        aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgc                                                        >  1:3905714/1‑101 (MQ=255)


                                        aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgc                                                        >  1:1772247/1‑101 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgt                                                      >  1:1441083/1‑99 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgt                                                      >  1:213205/1‑99 (MQ=255)


                                           gATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtc                                                     >  2:1906282/1‑98 (MQ=255)


                                            aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtct                                                    <  1:2841644/101‑5 (MQ=255)


                                              cAGCGTTGGGTATACCATGTACCAGCCGCTGGGCGCCTCCGAGCGCGCCCGCCGCCGGGTTCGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  2:298499/101‑1 (MQ=255)


                                               aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.g                                                <  1:3432045/101‑1 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:102203/1‑96 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  2:114582/1‑96 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:780780/1‑96 (MQ=255)


                                                       gcgtaCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:1947392/97‑1 (MQ=255)


                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  1:3758043/1‑101 (MQ=255)


                                                           aCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGc                                     >  2:1570293/1‑101 (MQ=255)


                                                            ccATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  2:4041419/101‑1 (MQ=255)


                                                            ccATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  1:1382919/101‑1 (MQ=255)


                                                             cATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   <  1:1995482/101‑1 (MQ=255)


                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  2:1889650/1‑101 (MQ=255)


                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCCGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                >  1:3707211/1‑101 (MQ=255)


                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTATATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                >  2:1392612/1‑101 (MQ=255)


                                                                 aaCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAtt                               >  1:1921199/1‑101 (MQ=255)


                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              >  1:1591926/1‑101 (MQ=255)


                                                                   gaaggtgatGGACGCCTTCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTg                             <  1:1798247/93‑1 (MQ=16)


                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           >  1:1532497/1‑101 (MQ=255)


                                                                       ccGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                         >  1:1489597/1‑101 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  1:1588928/101‑1 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  2:2034486/101‑1 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  1:3312648/101‑1 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  1:2305477/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  2:3171421/1‑101 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  1:262764/101‑1 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  2:331076/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  2:2411559/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  >  2:1795011/1‑101 (MQ=255)


                                                                               cgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 <  2:2093572/101‑1 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  2:4020005/1‑101 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  1:1023775/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  2:3254834/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  1:813614/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  1:2705874/101‑1 (MQ=255)


                                                                                     cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag           >  1:2058517/1‑101 (MQ=255)


                                                                                     cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag           >  1:642389/1‑101 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          <  2:128989/101‑1 (MQ=255)


                                                                                       aGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa         <  1:1358807/101‑1 (MQ=255)


                                                                                       aGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTGTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa         >  1:3606661/1‑101 (MQ=255)


                                                                                          gcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      <  2:1752443/97‑1 (MQ=255)


                                                                                           cgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     >  1:1077199/4‑101 (MQ=255)


                                                                                              ccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:1170164/101‑1 (MQ=255)


                                                                                              ccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:3729419/101‑1 (MQ=255)


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG