BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,057,830	+C	79.1%	intergenic (‑596/‑306)	RCAP_rcc01900 ← / → RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,057,830	1	.→C	79.1%	102.0 ‑0.9 ‑0.4	148	intergenic (‑596/‑306)	RCAP_rcc01900/RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand): new base (54/63): ref base (17/14): total (71/77)
Fisher's exact test strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.77e-01

Only 100 of 167 total aligned reads displayed.


GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.CGCG.CGC.A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913


gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgc                                                                                        >  1:4079464/1‑101 (MQ=255)


 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgcc                                                                                       <  1:332218/101‑2 (MQ=255)


 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgcc                                                                                       <  2:1895202/101‑2 (MQ=255)


 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgcc                                                                                       >  2:3515159/1‑100 (MQ=255)


  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgccg                                                                                      >  1:1166207/1‑99 (MQ=255)


  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.c.cg.cgccg                                                                                      <  2:3822589/101‑3 (MQ=255)


   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gc                                                                                   >  1:3507570/1‑101 (MQ=255)


   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gc                                                                                   >  2:3444635/1‑101 (MQ=255)


    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gca                                                                                  >  2:530425/1‑100 (MQ=255)


    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gca                                                                                  <  1:610276/101‑2 (MQ=255)


    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gca                                                                                  <  1:2563092/101‑2 (MQ=255)


     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  2:2581873/1‑99 (MQ=255)


     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  2:2633318/1‑99 (MQ=255)


     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaa                                                                                 >  1:2372506/1‑99 (MQ=255)


      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaag                                                                                <  1:267659/101‑4 (MQ=255)


      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaag                                                                                >  1:2073121/1‑98 (MQ=255)


       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagc                                                                               >  1:2599195/1‑97 (MQ=255)


       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagc                                                                               >  1:2668288/1‑97 (MQ=255)


        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcg                                                                              >  1:4138538/1‑96 (MQ=255)


         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgt                                                                             <  2:217910/101‑7 (MQ=255)


         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgt                                                                             >  1:2021460/1‑95 (MQ=255)


          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  1:143419/101‑8 (MQ=255)


          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  2:366527/101‑8 (MQ=255)


          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  1:298756/101‑8 (MQ=255)


          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            <  2:3021485/101‑8 (MQ=255)


          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgtt                                                                            >  1:505695/1‑94 (MQ=255)


           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.C.CG.CGC.C.Gcaagcgttt                                                                           >  1:930712/1‑93 (MQ=255)


                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCg                                                                     >  2:737565/1‑101 (MQ=255)


                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCg                                                                     >  2:289009/1‑101 (MQ=255)


                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGc                                                                   <  1:65878/101‑1 (MQ=255)


                      ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCa                                                                  <  2:3323212/101‑1 (MQ=255)


                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCAt                                                                 >  1:3987653/1‑101 (MQ=255)


                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCAt                                                                 >  1:336033/1‑101 (MQ=255)


                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAC.TAC.CCCGCCGCAA.GCGG.TT.CCGAACCGGCATc                                                                >  2:1560836/1‑101 (MQ=255)


                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATc                                                                >  2:2125239/1‑101 (MQ=255)


                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATc                                                                >  2:366991/1‑101 (MQ=255)


                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCa                                                               >  2:1679883/1‑101 (MQ=255)


                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCatt                                                             <  2:3502260/101‑3 (MQ=255)


                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCatt                                                             >  2:2765985/1‑99 (MQ=255)


                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CGCGCGCCGCAA.GC.GTTT.CCGTACCGGCATCatt                                                             >  2:380146/1‑99 (MQ=255)


                            tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTc                                                            >  2:3251354/1‑101 (MQ=255)


                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCt                                                           >  1:893358/1‑101 (MQ=255)


                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCt                                                           >  1:941540/1‑101 (MQ=255)


                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCt                                                           <  1:2842665/101‑1 (MQ=255)


                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCtt                                                          <  1:354524/101‑1 (MQ=255)


                                    cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.cccc                                                   >  2:3867871/1‑101 (MQ=255)


                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.cccccc                                                 <  1:145878/101‑2 (MQ=255)


                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.cccccc                                                 <  1:328166/101‑2 (MQ=255)


                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccccg                                                <  2:2372330/101‑3 (MQ=255)


                                          tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  1:3091032/101‑6 (MQ=255)


                                             gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                           >  2:2925284/1‑101 (MQ=255)


                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          >  2:3995263/1‑101 (MQ=255)


                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  1:1876978/101‑1 (MQ=255)


                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        <  1:1743740/101‑1 (MQ=255)


                                                 gggggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       <  1:1679998/98‑1 (MQ=255)


                                                  gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  1:2515319/101‑1 (MQ=255)


                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:505638/101‑1 (MQ=255)


                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   >  2:2692038/1‑101 (MQ=255)


                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:3098337/101‑1 (MQ=255)


                                                     cttccgatctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:827031/94‑1 (MQ=255)


                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:2417597/1‑101 (MQ=255)


                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:1319674/1‑101 (MQ=255)


                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 <  1:3787734/101‑1 (MQ=255)


                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:50404/1‑101 (MQ=255)


                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:930627/101‑1 (MQ=255)


                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:1515937/101‑1 (MQ=255)


                                                            tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            <  1:3034698/101‑1 (MQ=255)


                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:67894/1‑101 (MQ=255)


                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAT.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:3843217/101‑1 (MQ=255)


                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTcc                          >  1:822757/1‑100 (MQ=255)


                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          >  1:1175039/1‑101 (MQ=255)


                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:81802/1‑101 (MQ=255)


                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:975240/1‑101 (MQ=255)


                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCAAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  2:258077/101‑1 (MQ=255)


                                                                  gATCCTTCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GCTT.TT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  2:2056094/101‑1 (MQ=255)


                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:3824556/1‑101 (MQ=255)


                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3698311/1‑101 (MQ=255)


                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGC.ACGC.GTTT.CCGAACCGGCATCGTTCTTGGCCCCCCGCCGGGTCAATCACGGCCGCCTCATTGTTc                    <  1:3617281/101‑1 (MQ=255)


                                                                      cTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTccc                  >  2:3135731/1‑100 (MQ=255)


                                                                      cTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:2142521/1‑101 (MQ=255)


                                                                      cTGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  <  1:1592229/101‑1 (MQ=255)


                                                                       tGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATTATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:3958023/1‑101 (MQ=255)


                                                                       tGCGTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 <  1:2844047/101‑1 (MQ=255)


                                                                          gTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:3313142/1‑101 (MQ=255)


                                                                          gTTTCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              <  2:4138276/101‑1 (MQ=255)


                                                                          gTTTCCGGAGTGGCGACAA.TCC.CGCGCCGCAA.AC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCCCGGCCGCCTCATTGTTCCGCCAt              <  1:2035109/101‑1 (MQ=255)


                                                                           tttCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:3579780/101‑1 (MQ=255)


                                                                           tttCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:1253953/101‑1 (MQ=255)


                                                                            ttCCGGATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  1:27735/101‑1 (MQ=255)


                                                                            ttCCGGAGTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCAGCCATTg            <  1:1692159/101‑1 (MQ=255)


                                                                                ggATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:1549081/1‑101 (MQ=255)


                                                                                 gATTGGCGACAACC.C.CGCGCCGCAA.GC.G.TTCCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCAATTGCGATc       <  2:399230/101‑1 (MQ=255)


                                                                                 gATTGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCGATc       >  1:2374404/1‑101 (MQ=255)


                                                                                  aTTGGCTACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:1809245/101‑1 (MQ=255)


                                                                                   ttGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  2:12378/1‑101 (MQ=255)


                                                                                   ttGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  2:201029/101‑1 (MQ=255)


                                                                                    tGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:2720854/101‑1 (MQ=255)


                                                                                    tGGCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:2540734/101‑1 (MQ=255)


                                                                                     ggCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   >  2:3204027/1‑101 (MQ=255)


                                                                                      gCGACAA.TCC.CGCGCCGCAA.GC.GTTT.CCGACCCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:1867937/101‑1 (MQ=255)


GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.C.CGCG.CGC.A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG