Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,327 +C 3.9% intergenic (‑123/‑182) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3273.→C 3.9% 14.3 0.0 ‑0.0181intergenic (‑123/‑182)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (92/82):  total (96/85)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.72e-01

Only 100 of 205 total aligned reads displayed.

TTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAC  >  NC_014034/2249228‑2249426
                                                                                                      |                                                                                                             
ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGTCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                                <  1:1531353/101‑1 (MQ=255)
   agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                                          >  1:472561/1‑101 (MQ=255)
     aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTTTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                                      >  1:3603223/1‑101 (MQ=255)
     aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                                      >  2:1160157/1‑101 (MQ=255)
      tGCACAGATACCGCAGCTTGGGCACCCGGGGCATGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                     <  1:3660888/101‑1 (MQ=255)
          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                 <  2:3120736/101‑1 (MQ=255)
           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                <  2:3572785/101‑1 (MQ=255)
           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                <  2:2682658/101‑1 (MQ=255)
            gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                               >  1:136121/1‑101 (MQ=255)
            gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                               <  2:3723645/101‑1 (MQ=255)
              tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                                             <  2:2965172/101‑1 (MQ=255)
               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                            >  2:484617/1‑101 (MQ=255)
               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                            <  1:3145267/101‑1 (MQ=255)
               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                            >  1:2533207/1‑101 (MQ=255)
               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATCAA...GGGG..GGCTTTCATCt                                                                                            >  1:289967/1‑101 (MQ=255)
                ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                           <  1:2985079/101‑1 (MQ=255)
                  gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                         <  2:34017/101‑1 (MQ=255)
                     gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGa                                                                                      <  1:2398280/101‑1 (MQ=255)
                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                     <  1:2730742/101‑1 (MQ=255)
                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                    <  2:641662/101‑1 (MQ=255)
                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                   >  1:1777245/1‑101 (MQ=255)
                         gggGACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                  <  1:3101496/101‑1 (MQ=255)
                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                  <  1:1160251/101‑1 (MQ=255)
                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                  <  1:2869083/101‑1 (MQ=255)
                           gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                <  2:1046824/101‑1 (MQ=255)
                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                               <  1:4028292/101‑1 (MQ=255)
                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                               <  2:2549880/101‑1 (MQ=255)
                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                               <  2:2165523/101‑1 (MQ=255)
                              cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                             >  1:3493532/1‑101 (MQ=255)
                              cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                             <  2:1371457/101‑1 (MQ=255)
                               ccGGGGCAAGAAGGGGGCCCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.g                                                                           <  2:1997830/101‑1 (MQ=255)
                                cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.gg                                                                          >  2:2229856/1‑101 (MQ=255)
                                   ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.ggggg                                                                       >  2:3364408/1‑101 (MQ=255)
                                    gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.gggggg                                                                      <  2:1527196/101‑1 (MQ=255)
                                     cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGt                                                                     >  1:602512/1‑101 (MQ=255)
                                      aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTg                                                                    <  2:3329656/101‑1 (MQ=255)
                                      aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTg                                                                    <  2:3511322/101‑1 (MQ=255)
                                        gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGaa                                                                  <  2:911974/101‑1 (MQ=255)
                                          agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tg                                                               <  1:2616160/101‑1 (MQ=255)
                                           gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tgg                                                              <  1:119679/101‑1 (MQ=255)
                                           gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCAC.GGGGGGTGAA.Tgg                                                              >  1:450802/1‑101 (MQ=255)
                                             gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgcg                                                            >  1:2206728/1‑101 (MQ=255)
                                             gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgcg                                                            <  1:2264758/101‑1 (MQ=255)
                                              ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgcgc                                                           <  2:1721042/101‑1 (MQ=255)
                                               gCTCTGCCCCCGTCCCTTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCa                                                          <  2:1105257/101‑1 (MQ=255)
                                                ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..g                                                       <  1:106933/101‑1 (MQ=255)
                                                 tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..Gc                                                      >  1:4224026/6‑101 (MQ=255)
                                                  ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..Gcc                                                     <  1:1853884/97‑1 (MQ=255)
                                                   tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCa                                                    <  2:3211260/98‑1 (MQ=255)
                                                   tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCa                                                    >  1:3225845/4‑101 (MQ=255)
                                                    gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGCGCA..GCCag                                                   >  1:2243123/3‑101 (MQ=255)
                                                         cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAgggg                                              >  2:3683041/1‑101 (MQ=255)
                                                            ccgatctcGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                           <  2:425147/94‑1 (MQ=255)
                                                            cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                           >  2:3872364/1‑101 (MQ=255)
                                                              cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                         >  1:84139/1‑101 (MQ=255)
                                                              cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                         <  2:24645/101‑1 (MQ=255)
                                                              cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                         >  2:4102191/1‑101 (MQ=255)
                                                               gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                       <  1:1001720/101‑1 (MQ=255)
                                                               gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                       >  2:1123088/1‑101 (MQ=255)
                                                               gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                            >  2:4122634/7‑101 (MQ=21)
                                                                caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAggggggg                                           >  2:882955/6‑100 (MQ=18)
                                                                   cGGGACT.CCTCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                  <  2:459552/101‑1 (MQ=255)
                                                                   cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgatcgg                                    >  1:425204/1‑96 (MQ=255)
                                                                   cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                  >  1:1918090/1‑101 (MQ=255)
                                                                   cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                  >  1:995632/1‑101 (MQ=255)
                                                                   cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                  >  2:2919430/1‑101 (MQ=255)
                                                                    gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                 >  1:1616232/1‑101 (MQ=255)
                                                                    gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                 >  1:354411/1‑101 (MQ=255)
                                                                      gactccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.CG.cc                                   <  2:3320767/96‑1 (MQ=17)
                                                                         t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                            >  1:1710117/1‑97 (MQ=255)
                                                                          ccccccGGGTTTTTTGGGGGAAGAGGAA...GGGG..GGTTTTCATTTTGCCAAAAAAATCCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccctt                             <  1:1422384/100‑4 (MQ=21)
                                                                          ccccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                            <  2:2892488/100‑5 (MQ=255)
                                                                           cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                           <  2:3959378/101‑6 (MQ=255)
                                                                           cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                           <  2:450745/101‑6 (MQ=255)
                                                                            ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                           <  1:811842/101‑1 (MQ=255)
                                                                            ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                           <  2:1633285/101‑1 (MQ=255)
                                                                            ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                           >  1:2354331/1‑101 (MQ=255)
                                                                              ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgg                         >  1:82437/1‑101 (MQ=255)
                                                                               cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGAGAAT.TGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgacc                           >  2:1879142/1‑97 (MQ=2)
                                                                               cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccc                           >  2:1663196/1‑97 (MQ=0)
                                                                               cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcggg                        >  1:636164/1‑101 (MQ=255)
                                                                               cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcggg                        <  2:3162182/101‑1 (MQ=255)
                                                                                gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                       <  2:2916758/101‑1 (MQ=255)
                                                                                gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                       >  2:3783320/1‑101 (MQ=255)
                                                                                 ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggcg                      <  2:2533023/101‑1 (MQ=255)
                                                                                 gaatatTGGAAGAAAGATGAG...GGGG..TTTTTTCCTCGTGCAAAAAATCTCCG.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.CCCCCTTCgcgggcg                      <  1:762483/99‑1 (MQ=255)
                                                                                    tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                   >  2:1689121/1‑101 (MQ=255)
                                                                                     atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcg                  >  1:3698587/1‑101 (MQ=255)
                                                                                      tttGAGGCAAGATGAA...GGGG..GCCTTTCATCTTGCCGAAAATACCCC.GGGGGGTGAA.TGGCGCA..GCCAAAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgc                 <  1:1258836/101‑1 (MQ=37)
                                                                                       ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCAG.CGCCCCCCTTCGCGGCCGGCcgcg                >  2:1268224/1‑101 (MQ=37)
                                                                                         gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc              <  2:1972989/101‑1 (MQ=255)
                                                                                           ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc            >  2:370932/1‑101 (MQ=255)
                                                                                           ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc            >  1:910587/1‑101 (MQ=255)
                                                                                            gCAAGGTGGA...GGGG..GGCTCTCATCGTGCCGAAAATGCCCC.GGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcg           <  1:1681655/101‑1 (MQ=25)
                                                                                             cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAGAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc          >  1:3765924/1‑101 (MQ=255)
                                                                                              aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgcg         >  2:1837501/1‑101 (MQ=255)
                                                                                               aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc        >  2:1588134/1‑101 (MQ=255)
                                                                                                 aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg      >  2:3618452/1‑101 (MQ=255)
                                                                                                   gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa    <  1:1461113/101‑1 (MQ=255)
                                                                                                     a...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc  <  2:2262820/101‑1 (MQ=255)
                                                                                                      |                                                                                                             
TTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAC  >  NC_014034/2249228‑2249426

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG