Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,076 +C 11.6% intergenic (+124/‑118) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0760.→C11.6% 31.9 ‑0.3 ‑0.6116intergenic (+124/‑118)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (59/43):  total (66/50)
Fisher's exact test strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.39e-01

Only 100 of 133 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170
                                                                                                                                                                                                          
aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                                                 <  1:1217599/101‑1 (MQ=18)
 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCag                                                                                                <  2:1980703/101‑2 (MQ=18)
   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcg                                                                                              >  1:262851/1‑98 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                                                                                             >  2:4233939/1‑97 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaccgc                                                                                             >  1:1861491/1‑97 (MQ=17)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cc                                                                                          >  2:3495375/1‑101 (MQ=17)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                                                                                         >  2:181846/1‑101 (MQ=17)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                                        <  2:1471921/101‑1 (MQ=17)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                                        >  2:231394/1‑101 (MQ=18)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                                       >  2:633975/1‑101 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                                       >  2:321368/1‑101 (MQ=18)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                     <  1:4113550/101‑3 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                     >  2:3155734/1‑99 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                     <  2:640139/101‑3 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                     <  2:936538/101‑3 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.acccccg                                                                                     >  2:2285455/1‑99 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGccc                                                                                  <  1:3748899/101‑1 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                                                 <  2:3008822/101‑1 (MQ=17)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg                                                                                >  1:176553/1‑101 (MQ=2)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                                                >  1:1411281/1‑101 (MQ=17)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                                               <  1:76569/101‑2 (MQ=0)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                                               >  1:286327/1‑100 (MQ=2)
                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                                               >  2:3035939/1‑101 (MQ=18)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                              >  2:2911175/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                              >  1:1063102/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                              <  1:2038261/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                                              >  2:2615727/1‑101 (MQ=18)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                                                                              >  1:1983187/1‑101 (MQ=14)
                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.ACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                                                                            >  1:3675887/1‑101 (MQ=2)
                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                            >  1:3984679/1‑101 (MQ=17)
                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                                          <  2:3744366/101‑3 (MQ=2)
                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                                          <  1:582313/101‑3 (MQ=2)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                         >  1:3235319/1‑101 (MQ=17)
                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                      <  2:1717607/101‑1 (MQ=17)
                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                                                                     <  2:1059460/101‑1 (MQ=17)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                                                                    >  1:143678/1‑100 (MQ=17)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  >  1:4224779/1‑101 (MQ=17)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  <  1:2538613/101‑1 (MQ=17)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  >  2:625069/1‑101 (MQ=18)
                                aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                                                <  1:3195103/101‑1 (MQ=17)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGcc                                                              >  2:1265273/1‑101 (MQ=17)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                              >  1:335947/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt                                                             >  2:911065/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             <  1:3680930/101‑1 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGGGGCGGCGGCt                                                             >  1:2207444/1‑101 (MQ=9)
                                     aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg                                                           >  1:932663/1‑101 (MQ=9)
                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          >  1:2841566/1‑101 (MQ=17)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  1:882183/101‑1 (MQ=17)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc                                                         >  1:3622924/1‑101 (MQ=17)
                                         gATGTCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCG..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       <  2:1353657/101‑1 (MQ=18)
                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       <  2:2298375/101‑1 (MQ=17)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     <  2:1784540/101‑1 (MQ=17)
                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                                    <  2:2046365/101‑1 (MQ=17)
                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   <  1:1165216/101‑1 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  >  2:3396601/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCTGTGACGGGCgggg                                                 >  2:3060860/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 >  2:3468148/1‑101 (MQ=18)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGCGGCGGCGCCTTGACGGGCggggc                                                >  2:3271940/1‑100 (MQ=17)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               >  2:1293961/1‑101 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               >  2:1319747/1‑101 (MQ=18)
                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGCGGGCGGGGGcgcg                                            >  2:1136849/1‑101 (MQ=18)
                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  2:262806/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  1:2176365/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  2:3159051/101‑1 (MQ=18)
                                                             aagtgtCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   <  1:3190945/96‑1 (MQ=18)
                                                               atatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 <  2:1554652/101‑1 (MQ=21)
                                                                tatCCGGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                <  2:1519688/101‑1 (MQ=21)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  2:1783617/1‑101 (MQ=21)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  1:3055170/1‑101 (MQ=21)
                                                                 atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGACGGGGGGGGGCGCGGGCAGTAACCCaa                               >  1:640583/1‑101 (MQ=12)
                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              >  1:1570566/1‑101 (MQ=21)
                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              >  2:2713553/1‑101 (MQ=21)
                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             <  2:2775235/101‑1 (MQ=21)
                                                                     cGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg                           >  2:1127724/1‑101 (MQ=25)
                                                                      ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          <  1:4170531/101‑1 (MQ=25)
                                                                      ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          >  1:1912598/1‑101 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:4162475/1‑101 (MQ=25)
                                                                       gggggTGAA.AGGC..GAAG.CCA..GAGGGGGGCAGCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTTACCGAAATTGCa                         <  1:2285623/101‑1 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAGCCAG...AGGGGGTC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:2414711/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:3179071/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  2:832539/1‑101 (MQ=25)
                                                                         gggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  1:1611537/1‑101 (MQ=25)
                                                                           gTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  1:592488/1‑101 (MQ=25)
                                                                                        gcgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:1782045/91‑1 (MQ=38)
                                                                                         cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  1:4234201/92‑1 (MQ=37)
                                                                                         cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             >  2:4149892/10‑101 (MQ=38)
                                                                                         cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  2:1615623/92‑1 (MQ=38)
                                                                                          gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  1:3338605/9‑101 (MQ=38)
                                                                                           aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:2928861/8‑101 (MQ=38)
                                                                                              ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:2936686/5‑101 (MQ=38)
                                                                                              ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:87542/97‑1 (MQ=38)
                                                                                                agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTTCTTGGGGGCGGCTTACAGGGAGGGGGCGCCGGCTTTTACCGACATTTCAACCGCCGCTGCTATccacc      >  2:3599750/3‑98 (MQ=2)
                                                                                                agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:2504183/3‑101 (MQ=39)
                                                                                                agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1177481/3‑101 (MQ=39)
                                                                                                agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1928907/3‑101 (MQ=39)
                                                                                                 gaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  2:1431756/2‑101 (MQ=39)
                                                                                                  aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:1646855/1‑101 (MQ=255)
                                                                                                  aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:1168432/1‑101 (MQ=255)
                                                                                                    gggggCAGGCC.CCCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  <  1:1990499/101‑1 (MQ=255)
                                                                                                    gggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:261335/1‑101 (MQ=255)
                                                                                                                                                                                                          
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG