BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_014034	996,774	+AACCGCGGCGAGATCGCC	100%	coding (24/1986 nt)	pccA →	propionyl‑CoA carboxylase subunit alpha

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	996768 =	0 (0.000)	208 (1.100)	121/178	0.0	0.97	coding (18/1986 nt)	pccA	propionyl‑CoA carboxylase subunit alpha
*	?	NC_014034	= 996774	13 (0.060)	208 (1.100)	121/178	0.0	0.97	coding (24/1986 nt)	pccA	propionyl‑CoA carboxylase subunit alpha

Only 100 of 470 total aligned reads displayed.


CGTTTCGTTTCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCCG  >  NC_014034/996668‑996868


ttgacggcacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgag                                                                                                      >  1:2080043‑M1/101‑101 (MQ=255)


cgtttcgttcCCCCCGCCTTGACGGCACATGACTTCACGAAAGGGGGCGCGGGCCAACCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTg                                                                                                      <  1:3613896/101‑1 (MQ=255)


 gtttcgtttcGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGa                                                                                                     >  1:158025/1‑101 (MQ=255)


  tttcgtttcgCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGAt                                                                                                    >  2:778893/1‑101 (MQ=255)


  tttcgtttcgCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGAt                                                                                                    >  2:13856/1‑101 (MQ=255)


  tttcgtttccCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGACGGGACTGACCATGTTCAAGAAAATCCTGAt                                                                                                    <  1:3762927/101‑1 (MQ=255)


  ttctgtttcgCCCCCCCTGACGGCACATGACAGCAGGAAAGGGGGCGGGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGAt                                                                                                    <  1:2383256/97‑1 (MQ=255)


    cggcacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCg                                                                                                  <  1:53248‑M1/5‑1 (MQ=255)


      gttccgCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGcc                                                                                                <  2:1859405/101‑1 (MQ=255)


       tttCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCa                                                                                               <  2:364509‑M2/101‑2 (MQ=255)


       cacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCt                                                                                               >  1:4228827‑M1/94‑101 (MQ=255)


       cacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCt                                                                                               >  2:4021153‑M1/94‑101 (MQ=255)


        ttCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaa                                                                                              <  1:1038328‑M2/101‑3 (MQ=255)


        acatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTg                                                                                              <  1:475122‑M1/9‑1 (MQ=255)


         catgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGc                                                                                             >  2:2576023‑M1/92‑101 (MQ=255)


           tgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCcg                                                                                           <  1:3132262‑M1/12‑1 (MQ=255)


            cccgccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgc                                                                                          <  1:1920799‑M2/101‑7 (MQ=255)


             ccgccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcg                                                                                         >  1:3700897‑M2/1‑94 (MQ=255)


              cgccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcgg                                                                                        <  1:1512607‑M2/101‑9 (MQ=255)


              catcacgaaagggggcgcgggcaaatccgcgcccggaggaaggggctgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGccgcgc                                                                                        <  2:2804963‑M1/15‑2 (MQ=255)


               gccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggc                                                                                       >  2:124682‑M2/1‑92 (MQ=255)


               atcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGtc                                                                                       <  1:392330‑M1/16‑1 (MQ=255)


                 cTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcga                                                                                     <  2:1601033‑M2/101‑12 (MQ=255)


                  ttGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgag                                                                                    <  1:407118‑M2/101‑13 (MQ=255)


                  acgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGtcatc                                                                                    >  1:3419991‑M1/83‑101 (MQ=255)


                   cgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGtcatca                                                                                   <  1:4184482‑M1/20‑1 (MQ=255)


                    gaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCaa                                                                                  <  2:1219721‑M1/21‑1 (MQ=255)


                    gaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCaa                                                                                  <  2:488912‑M1/21‑1 (MQ=255)


                       agggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCTCGTCATCAAATc                                                                               <  1:3353500‑M1/24‑1 (MQ=255)


                       agggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATc                                                                               <  1:1672310‑M1/24‑1 (MQ=255)


                         ggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCgg                                                                             <  1:2142050‑M1/26‑1 (MQ=255)


                                cATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgt                                                                      >  1:724835‑M2/1‑75 (MQ=255)


                                cATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgt                                                                      <  1:1605301‑M2/101‑27 (MQ=255)


                                 ggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAg                                                                     >  2:2964051‑M1/68‑101 (MQ=255)


                                   cACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcat                                                                   >  1:3820294‑M2/1‑72 (MQ=255)


                                    caatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATg                                                                  >  1:3008247‑M1/65‑101 (MQ=255)


                                     cGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatca                                                                 >  2:585817‑M2/1‑70 (MQ=255)


                                       tccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGc                                                               >  2:4197181‑M1/62‑101 (MQ=255)


                                        ccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGCTGGGCa                                                              <  1:672922‑M1/41‑1 (MQ=255)


                                        ccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCa                                                              <  2:330198‑M1/41‑1 (MQ=255)


                                         cgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCAt                                                             >  1:1683814‑M1/60‑101 (MQ=255)


                                         cgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCAt                                                             <  1:3399590‑M1/42‑1 (MQ=255)


                                         aGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatc                                                             <  2:1734523‑M2/101‑36 (MQ=255)


                                          gggggCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcg                                                            <  2:1077520‑M2/101‑37 (MQ=255)


                                             ggCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcg                                                         >  1:2730624‑M2/1‑62 (MQ=255)


                                             cccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAg                                                         <  1:1025913‑M1/46‑1 (MQ=255)


                                                 gaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACgg                                                     <  2:1514136‑M1/50‑1 (MQ=255)


                                                  gggCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaa                                                    <  2:3362742‑M2/101‑45 (MQ=255)


                                                  aggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGt                                                    <  2:1920750‑M1/51‑1 (MQ=255)


                                                  aggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGt                                                    >  2:1349764‑M1/51‑101 (MQ=255)


                                                    gCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaaga                                                  <  1:2517732‑M2/101‑47 (MQ=255)


                                                     ccAATCCGCCCCCGGAGGAAGGGACTGACCATTTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagat                                                 >  1:3080847‑M2/1‑54 (MQ=255)


                                                      agggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGcc                                                >  2:2598016‑M1/47‑101 (MQ=255)


                                                      agggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGcc                                                <  2:3182959‑M1/55‑1 (MQ=255)


                                                       gggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGgccn                                               >  1:294161‑M1/46‑100 (MQ=255)


                                                       gggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCAGCGCGCAAGATGGGCATCAAGACGGTGGCCg                                               <  2:3050656‑M1/56‑1 (MQ=255)


                                                          actgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCt                                            <  1:3533389‑M1/59‑1 (MQ=255)


                                                            tgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTAt                                          <  2:1836419‑M1/61‑1 (MQ=255)


                                                             gaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTAtt                                         <  2:527192‑M1/62‑1 (MQ=255)


                                                              gcCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaa                                        <  1:560801‑M2/101‑57 (MQ=255)


                                                               cccGGAGGAAGGGACTGACCAGGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaag                                       >  2:1672195‑M2/1‑44 (MQ=255)


                                                                ccGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaaga                                      >  1:1701416‑M2/1‑43 (MQ=255)


                                                                ccGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaaga                                      >  1:498498‑M2/1‑43 (MQ=255)


                                                                ccGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaaga                                      >  2:475065‑M2/1‑43 (MQ=255)


                                                                catgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccg                                      <  1:2641147‑M1/65‑1 (MQ=255)


                                                                  ggaggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacg                                    >  1:1403408‑M2/1‑41 (MQ=255)


                                                                   gttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatg                                   <  2:4058283‑M1/68‑1 (MQ=255)


                                                                    aggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggt                                  <  2:1424470‑M2/101‑63 (MQ=255)


                                                                     tcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgcc                                 >  1:1210359‑M1/32‑101 (MQ=255)


                                                                     ggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagacgggcatcaagacggtg                                 <  1:3428102‑M2/101‑64 (MQ=255)


                                                                      gaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtgg                                >  2:2860045‑M2/1‑37 (MQ=255)


                                                                      caagaaaagcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgccg                                >  2:3634771‑M1/31‑101 (MQ=255)


                                                                        agaaaatcctgatcgccaaccgcggcgaGATGGCCTGCCGGGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGGGGCCGTCTATTccgatgccgat                              <  1:3213387‑M1/73‑1 (MQ=255)


                                                                         gggACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccg                             <  1:4106906‑M2/101‑68 (MQ=255)


                                                                          ggACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgt                            <  1:2227387‑M2/101‑69 (MQ=255)


                                                                          aaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCcgatgccgatgc                            <  1:2682833‑M1/75‑1 (MQ=255)


                                                                           gACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtc                           <  2:3096290‑M2/101‑70 (MQ=255)


                                                                           aaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATgcg                           <  1:547062‑M1/76‑1 (MQ=255)


                                                                           aaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATgcg                           <  2:2430452‑M1/76‑1 (MQ=255)


                                                                            aCTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtct                          <  1:3251283‑M2/101‑71 (MQ=255)


                                                                            aCTGACCATGTTCAAGAAAATCCTCATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtct                          >  2:2517548‑M2/1‑31 (MQ=255)


                                                                             atcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCa                         >  2:248945‑M1/24‑101 (MQ=255)


                                                                              tGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctat                        <  1:1305339‑M2/101‑73 (MQ=255)


                                                                               cctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCACAgg                       <  2:2629905‑M1/80‑1 (MQ=255)


                                                                                ctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGgc                      <  1:1244692‑M1/81‑1 (MQ=255)


                                                                                ctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGgc                      <  1:1255955‑M1/81‑1 (MQ=255)


                                                                                aCCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattc                      >  2:3676649‑M2/1‑27 (MQ=255)


                                                                                  gatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGACGTCTATTCCGGTGCCGATGCGCAGgcgc                    <  2:1059710‑M1/83‑1 (MQ=255)


                                                                                   atcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCt                   >  1:814926‑M1/18‑101 (MQ=255)


                                                                                      ttCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgc                <  1:281369‑M2/101‑81 (MQ=255)


                                                                                      gccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCa                <  2:3887189‑M1/87‑1 (MQ=255)


                                                                                        cAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccg              >  2:392279‑M2/1‑19 (MQ=255)


                                                                                        cAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccg              <  2:2081454‑M2/101‑83 (MQ=255)


                                                                                         aaGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccga             <  2:2666678‑M2/101‑84 (MQ=255)


                                                                                             gcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGaaa         >  1:4020825‑M1/8‑101 (MQ=255)


                                                                                              aaTCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgc        <  2:2278584‑M2/101‑89 (MQ=255)


                                                                                               aTCCTGATCGCCacccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgca       >  2:3012931‑M2/1‑12 (MQ=255)


                                                                                                   tGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcaggcg   >  2:2241931‑M2/1‑8 (MQ=255)


                                                                                                    gATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcaggcgc  <  2:2166997‑M2/101‑95 (MQ=255)


                                                                                                    gATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCcg  >  2:3747910/1‑101 (MQ=255)


CGTTTCGTTTCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCCG  >  NC_014034/996668‑996868

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG