Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,7180C→G100.0% 1.9 4intergenic (‑497/‑461)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (0/0):  total (0/4)

TTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434674‑1434778
                                            |                                                              
tcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATc        <  2:1766922/95‑1 (MQ=2)
tcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:2672350/95‑1 (MQ=2)
     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac   <  1:2016934/100‑1 (MQ=2)
      gTCACTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca  <  1:3797446/101‑2 (MQ=2)
                                            |                                                              
TTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434674‑1434778

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG