BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,956	T→C	16.3%	F280L (TTC→CAA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,956	0	T→C	16.3%	102.8 ‑0.3 ‑1.4	278	F280L (TTC→CTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/22): ref base (108/111): total (135/143)
Fisher's exact test strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.66e-02

Only 100 of 307 total aligned reads displayed.


GTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396856‑1397056


gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                           >  1:4043265/1‑101 (MQ=255)


 tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt.t                                                                                                         <  2:3478967/101‑1 (MQ=255)


 tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt.t                                                                                                         >  2:639601/1‑101 (MQ=255)


 tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt.t                                                                                                         >  1:3165635/1‑101 (MQ=255)


    aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.Tccc                                                                                                      >  1:1108784/1‑100 (MQ=255)


     tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc                                                                                                     >  1:859960/1‑101 (MQ=255)


      cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                   >  1:3749821/1‑101 (MQ=255)


      cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                   <  2:4030411/101‑1 (MQ=255)


       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                  <  1:1369072/101‑1 (MQ=255)


       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                  <  1:3280969/101‑1 (MQ=255)


       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                  <  2:1847080/101‑1 (MQ=255)


        aaaCCGGTCCAGAGATAGACCGCGCGTACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                 <  1:2547354/101‑1 (MQ=255)


         acccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                              <  2:3899835/99‑1 (MQ=255)


         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                              >  1:589947/1‑101 (MQ=255)


         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                              <  2:2637928/101‑1 (MQ=255)


         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                              <  2:2734588/101‑1 (MQ=255)


            cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg                                                                                           >  2:1126893/1‑101 (MQ=255)


              gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.Gt                                                                                        >  1:691337/1‑101 (MQ=255)


                      aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                                >  2:3204558/1‑101 (MQ=255)


                       tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                               <  2:824837/101‑1 (MQ=255)


                       tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                               >  1:2162195/1‑101 (MQ=255)


                        aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                              <  1:1970019/101‑1 (MQ=255)


                            cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                          <  2:701552/101‑1 (MQ=255)


                             gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                         >  2:2183098/1‑101 (MQ=255)


                               gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                       >  2:1222752/1‑101 (MQ=255)


                                 ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                     <  1:1080464/101‑1 (MQ=255)


                                  gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                                    <  1:1601367/99‑1 (MQ=255)


                                   aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:432769/101‑1 (MQ=255)


                                   aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:2849425/101‑1 (MQ=255)


                                     ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  2:281490/101‑1 (MQ=255)


                                     ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:2127599/101‑1 (MQ=255)


                                     attggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:1786308/91‑1 (MQ=0)


                                      ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  1:4226500/10‑101 (MQ=2)


                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  2:1578004/1‑101 (MQ=255)


                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  1:1018959/1‑101 (MQ=255)


                                       ccccgcccGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:268214/94‑1 (MQ=0)


                                        ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:1612696/94‑1 (MQ=2)


                                        cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:1343515/95‑1 (MQ=2)


                                        caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:2465886/94‑1 (MQ=9)


                                         ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:412144/6‑101 (MQ=2)


                                         ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:521938/96‑1 (MQ=2)


                                         aacactgTCGCTCCCCCCGCGGGGGGGTGGATCGAAAC..GCGG.GCC.CGCGC.CGCG.CCGGATCTTTGT.CGG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAttg                                                              >  1:4135029/7‑99 (MQ=2)


                                         aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:3375370/95‑1 (MQ=9)


                                          gttgcggcGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  1:1921921/8‑101 (MQ=2)


                                          gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:2234409/97‑1 (MQ=2)


                                          gttgcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:4090654/97‑1 (MQ=2)


                                          cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:764498/97‑1 (MQ=2)


                                          ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:2289869/6‑101 (MQ=2)


                                          acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCCC.GC.CGCG.CCAGATCTTTGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                             <  2:1965481/94‑1 (MQ=2)


                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  2:3598272/6‑101 (MQ=9)


                                           aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  2:1681531/1‑101 (MQ=255)


                                             gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:2561397/100‑1 (MQ=2)


                                             gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  1:3900838/100‑1 (MQ=2)


                                             cgggcGCCCCCCCCGCGGGGGCGTGGATCGNAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                         <  1:2799515/97‑2 (MQ=2)


                                             aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                         >  2:3712400/1‑101 (MQ=255)


                                              tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                         <  2:2383205/100‑2 (MQ=9)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                      <  1:363515/101‑1 (MQ=255)


                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                                      >  2:1906603/1‑101 (MQ=255)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                        <  2:96623/101‑2 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                        >  2:1426685/1‑100 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccc                                                       >  2:3421640/1‑97 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                    >  1:3714607/1‑101 (MQ=255)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                    >  2:4172414/1‑95 (MQ=2)


                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGG.CG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                                      >  2:2610838/1‑96 (MQ=2)


                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                    <  2:3087523/101‑7 (MQ=2)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                  >  1:1045238/1‑101 (MQ=255)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                   >  1:3920103/1‑94 (MQ=9)


                                                    cccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                   <  1:1246585/100‑8 (MQ=9)


                                                     ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                    <  1:1693580/101‑3 (MQ=2)


                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                  <  1:1551679/101‑9 (MQ=9)


                                                       ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                                >  2:471760/1‑91 (MQ=2)


                                                        cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                              <  1:351593/101‑1 (MQ=255)


                                                          cgggggggCGTGGATCGAAACG.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                            <  1:3097644/98‑1 (MQ=255)


                                                          cgcgGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                               >  1:910829/1‑94 (MQ=0)


                                                          cgcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                            <  2:2427774/101‑1 (MQ=255)


                                                           gcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGTCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                           <  2:206652/101‑1 (MQ=255)


                                                            cgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                          <  2:2891677/101‑1 (MQ=255)


                                                             gggggCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                         >  2:1269658/1‑101 (MQ=255)


                                                               gggCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                       <  2:3165396/101‑1 (MQ=255)


                                                                 gCGTGGATCGAACCA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTGGCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                     <  1:4178471/101‑1 (MQ=255)


                                                                 gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                     <  2:1797123/101‑1 (MQ=255)


                                                                 gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                     <  2:2961625/101‑1 (MQ=255)


                                                                   gTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                   >  2:1088904/1‑101 (MQ=255)


                                                                      gagcGAAACA.TCCT.GCG.CG.GC.CGCA.TCTGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                <  1:3373219/98‑1 (MQ=255)


                                                                      gATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                <  2:3314361/101‑1 (MQ=255)


                                                                          gAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTCGTCAGCCGGACGATg                            >  1:476185/1‑101 (MQ=255)


                                                                           aaaCA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                           >  1:769828/1‑101 (MQ=255)


                                                                           aaaCA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                           >  1:2899939/1‑101 (MQ=255)


                                                                              cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGGCGATGTCCg                        >  2:381142/1‑101 (MQ=255)


                                                                              cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                        <  1:3945236/101‑1 (MQ=255)


                                                                              cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTACg                        <  2:3518471/101‑1 (MQ=255)


                                                                               a.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt                       >  2:1068396/1‑101 (MQ=255)


                                                                                 gCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                      >  1:1747365/1‑101 (MQ=255)


                                                                                    t.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                   <  2:3257704/101‑1 (MQ=255)


                                                                                        g.cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                <  2:1358614/101‑1 (MQ=255)


                                                                                             gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc             <  1:1410445/101‑1 (MQ=255)


                                                                                                 gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATAAGCTCGTCGTTGGTCAGCAGGACGATGTCCGTCGCTCCCCCcgcg          >  1:3555133/1‑101 (MQ=255)


                                                                                                      cGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCCCCCCCCGCggggg      >  2:334141/1‑101 (MQ=255)


                                                                                                       ggCT.TCCGC.GTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc     <  2:952332/101‑1 (MQ=255)


                                                                                                         cT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCgg   >  2:544704/1‑100 (MQ=255)


GTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396856‑1397056

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG