BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,960	G→T	10.2%	R281L (CGC→TTT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,960	0	G→T	10.2%	70.5 0.0 ‑0.6	283	R281L (CGC→CTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (14/15): ref base (122/132): total (136/147)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.75e-01

Only 100 of 307 total aligned reads displayed.


TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396861‑1397060


 cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                                       >  1:3749821/1‑101 (MQ=255)


 cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                                       <  2:2861881/101‑1 (MQ=255)


        ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CCG..CGTg.g                                                                                             >  1:2891896/1‑101 (MQ=255)


          gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                                           <  1:981186/100‑1 (MQ=255)


            cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGc                                                                                         >  2:417198/1‑101 (MQ=255)


                 aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GGCGCTcccc                                                                                    >  2:3944976/1‑101 (MQ=255)


                 aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GCCGCTcccc                                                                                    >  2:3472049/1‑101 (MQ=255)


                  tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                                                   <  2:824837/101‑1 (MQ=255)


                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                                                   >  1:2162195/1‑101 (MQ=255)


                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                                                   >  1:1797242/1‑101 (MQ=255)


                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                                  <  1:169125/101‑1 (MQ=255)


                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                                  <  2:4043004/101‑1 (MQ=255)


                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                                  <  1:2825086/101‑1 (MQ=255)


                       cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                              <  2:701552/101‑1 (MQ=255)


                       cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGCG.GTCGCTCCCCCCGCgg                                                                              >  2:2032607/1‑101 (MQ=255)


                           cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          >  2:1772284/1‑101 (MQ=255)


                           cgGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          <  2:1882480/101‑1 (MQ=255)


                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                         <  1:1080464/101‑1 (MQ=255)


                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                         >  1:4180781/1‑101 (MQ=255)


                                tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  2:2948618/92‑1 (MQ=0)


                                ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:2127599/101‑1 (MQ=255)


                                 ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:777828/92‑1 (MQ=2)


                                 tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  1:1018959/1‑101 (MQ=255)


                                  tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:875586/93‑1 (MQ=2)


                                  tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:2628118/93‑1 (MQ=2)


                                  gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  1:1291637/8‑101 (MQ=2)


                                   ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:3232107/94‑1 (MQ=2)


                                   cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGaag                                                                   >  2:184614/7‑99 (MQ=2)


                                   cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:2627926/7‑101 (MQ=2)


                                   cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:3268080/95‑1 (MQ=2)


                                    ggttgcGTCGCTCCACCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:1072222/96‑1 (MQ=2)


                                    ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:1557810/96‑1 (MQ=2)


                                     gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:2234409/97‑1 (MQ=2)


                                     cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:764498/97‑1 (MQ=2)


                                     ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  2:1141088/6‑101 (MQ=2)


                                     ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  2:2439711/96‑1 (MQ=2)


                                     ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  2:2243330/96‑1 (MQ=2)


                                     ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  1:2289869/6‑101 (MQ=2)


                                     cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  1:3035506/1‑101 (MQ=255)


                                     acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCCC.GC.CGCG.CCAGATCTT.TGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                                 <  2:1965481/94‑1 (MQ=2)


                                      gaccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  2:1579462/4‑101 (MQ=255)


                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:3900838/100‑1 (MQ=2)


                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             >  2:2687360/3‑100 (MQ=2)


                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             <  2:594928/99‑2 (MQ=2)


                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAct                                                             >  2:1225835/3‑100 (MQ=2)


                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            >  2:2609413/2‑99 (MQ=2)


                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            >  1:1722476/2‑99 (MQ=2)


                                         ggTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            >  1:549536/2‑99 (MQ=2)


                                         cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                            >  1:1358714/1‑101 (MQ=255)


                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:1369533/1‑101 (MQ=2)


                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                           <  2:3711751/101‑1 (MQ=255)


                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          <  1:363515/101‑1 (MQ=255)


                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                          <  1:7030/101‑5 (MQ=2)


                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                           <  1:3387187/101‑4 (MQ=9)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  2:3356722/101‑2 (MQ=2)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  2:444527/101‑2 (MQ=2)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  2:96623/101‑2 (MQ=2)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                                            >  2:3513049/1‑100 (MQ=2)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                          >  1:1551124/1‑97 (MQ=9)


                                            cGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                                         <  2:3315692/101‑1 (MQ=255)


                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                         >  2:3732868/1‑96 (MQ=2)


                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                        >  1:3714607/1‑101 (MQ=255)


                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                        >  2:1309375/1‑95 (MQ=2)


                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                       <  2:1634861/101‑8 (MQ=2)


                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                      >  1:1045238/1‑101 (MQ=255)


                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                      >  2:2547856/1‑101 (MQ=255)


                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       >  2:505930/1‑94 (MQ=9)


                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       >  1:3920103/1‑94 (MQ=9)


                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       >  1:3196182/1‑94 (MQ=9)


                                               cccccccGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                      <  2:1102975/100‑9 (MQ=2)


                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                      <  1:1972157/101‑9 (MQ=9)


                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                      >  1:4230925/1‑93 (MQ=9)


                                                 cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                    <  2:3391650/101‑1 (MQ=255)


                                                 cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCG.CC.GC.AGCG.AGTGCG.TCTTGA.ACA..GGAT.GTCGCTCCCCCCGCAGGGGCGTGGATCGAAActatgctggc                                                     >  1:3551874/1‑92 (MQ=2)


                                                  ccccGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                                    >  1:49656/1‑91 (MQ=2)


                                                   cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                                                  >  1:4113510/1‑101 (MQ=255)


                                                     cgcgGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                   <  1:1934933/101‑8 (MQ=0)


                                                        gggggCGGGGAACGAAACA.GCCG.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                             <  2:3097959/101‑1 (MQ=255)


                                                                ggATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  1:641595/1‑101 (MQ=255)


                                                                  aTCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                   <  2:3891940/101‑1 (MQ=255)


                                                                    cGAAGCA.GCCG.GCG.CG.GC.CGCA.TCTGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 <  2:2401862/101‑1 (MQ=255)


                                                                         cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                            <  1:3945236/101‑1 (MQ=255)


                                                                              cT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                        >  2:2782294/1‑101 (MQ=255)


                                                                                 gcg.cG.GC.CGCA.GCGGCG.TC.CGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                      <  1:3296079/101‑1 (MQ=255)


                                                                                   g.cg.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                    <  2:2162050/101‑1 (MQ=255)


                                                                                         c.cGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg                >  1:2729697/1‑101 (MQ=255)


                                                                                         c.cGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCCGACGATGTCCGTCGCTccccccc                >  1:4079993/1‑100 (MQ=255)


                                                                                            gCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              >  1:1586632/1‑101 (MQ=255)


                                                                                             cA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg             >  1:139992/1‑101 (MQ=255)


                                                                                                  ggCT.TC.CGC.GTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc         <  2:952332/101‑1 (MQ=255)


                                                                                                    cT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGt       <  2:4212281/101‑1 (MQ=255)


                                                                                                    cT.TC.CGC.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGt       <  2:3572318/101‑1 (MQ=255)


                                                                                                     t.tC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg      >  1:4141334/1‑101 (MQ=255)


                                                                                                     t.tC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg      >  2:317413/1‑101 (MQ=255)


                                                                                                          cGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAt   >  1:1784257/1‑101 (MQ=255)


                                                                                                          cGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:1222848/101‑1 (MQ=255)


                                                                                                          cGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAt   <  2:3056871/101‑1 (MQ=255)


                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  1:3566638/101‑1 (MQ=255)


                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  1:1126987/101‑1 (MQ=255)


                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGCGGATc  >  1:2423691/1‑101 (MQ=255)


TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396861‑1397060

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG